Recombinant Human Secreted Frizzled-Related Protein 2 (SFRP2) Protein (His-GST)

Name: Recombinant Human Secreted Frizzled-Related Protein 2 (SFRP2) Protein (His-GST)
Brand: Beta LifeScience
SKU: BLC-07469P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Human Secreted Frizzled-Related Protein 2 (SFRP2) Protein (His-GST) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	Q96HF1
Target Symbol	SFRP2
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His-GST
Target Protein Sequence	TMKEVLEQAGAWIPLVMKQCHPDTKKFLCSLFAPVCLDDLDETIQPCHSLCVQVKDRCAPVMSAFGFPWPDMLECDRFPQDNDLCIPLASSDHLLPATEEAPKVCEACKNKNDDDNDIM
Expression Range	68-186aa
Protein Length	Partial
Mol. Weight	44.9 kDa
Research Area	Cancer
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis.
Subcellular Location	Secreted.
Protein Families	Secreted frizzled-related protein (sFRP) family
Database References	HGNC: 10777 OMIM: 604157 KEGG: hsa:6423 STRING: 9606.ENSP00000274063 UniGene: Hs.481022
Tissue Specificity	Expressed in adipose tissue, heart, brain, skeletal muscle, pancreas, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in adipose tissue, small intestine and colon.

Gene Functions References

SFRP2 was downregulated in Choriocarcinoma and low expressed SFRP2 could promote migration, invasion, the epithelial-mesenchymal transition phenotype and stemness of Choriocarcinoma cells via Wnt/beta-catenin signaling PMID: 30396168
Results show that SFRP2 and SFRP4 typically associate with a poor prognosis of cancer patients concomitant with the expression of genes associated with epithelial-to-mesenchymal transition. SFRP2 and 4 are likely derived from the tumor stroma, and thus tend to increase in tumors as compared to normal tissues. PMID: 28218291
The content of the methylated SFRP2 gene in endometrial tissue of patients with hyperplastic processes higher than 20-25% allows relate these women to the risk group of EC development and dictates the need of intensive observation of such patients. PMID: 29949535
Findings demonstrated that SFRP2 was downregulated in pituitary corticotroph adenomas. SFRP2 appears to act as a tumor suppressor in Cushing's disease by regulating the activity of the Wnt signaling pathway. PMID: 29620167
SFRP2 was down-regulated in the accelerated and blast phase of CML, whereas, the levels of WNT1, WNT3 and WNT5A were up-regulated in the accelerated and blast phase of CML. Overexpression SFRP2 inhibited proliferation, promoted apoptosis and activated the WNT pathway. PMID: 29704505
Methylation of SFRP2 gene may promote the invasiveness of non-small cell lung cancer cells through ZEB1 and MMP9 signaling. PMID: 29320940
the differences in SFRP2 promotor methylation observed between colorectal cancer (CRC)patients and healthy individuals by both assays were statistically significant (p<0.001). Our findings, although limited by the small sample size, do not support the use of the MSRH assay for CRC screening in stool. PMID: 28777432
augments Wnt3a-mediated beta-catenin signalling in dermal papilla cells, especially in beard dermal papilla cells PMID: 26914690
SFRP2 methylation is associated with colorectal cancer development. PMID: 26258809
TET1 potently inhibited canonical Wnt/beta-catenin signaling by demethylating and upregulating two upstream antagonists of this pathway, SFRP2 and DKK1, which was associated with inhibition of EMT and cancer cell metastasis. PMID: 28851501
SFRP2 could bind to locally present Wnt ligands and alter the balance of intracellular Wnt signaling to antagonize the canonical Wnt pathway in stem cells from the apical papilla. PMID: 28794794
SFRP2 induction is remarkable in tumor stroma, with transcription mainly modulated by the nuclear factor-kappaB (NF-kappaB) complex, a property shared by several effectors of the DNA damage secretory program. PMID: 26751775
down-regulated in ICU-acquired weakness and mice with inflammation-induced muscle atrophy; possibly establishes a positive feedback loop enhancing TGF-beta1-mediated atrophic effects in inflammation-induced atrophy PMID: 27661566
epigenetic silencing of Wnt antagonists was associated with gastric carcinogenesis, and concurrent hypermethylation of SFRP2 and DKK2 could be a potential marker for a prognosis of poor overall survival. PMID: 28152305
SFRP2 enhances the adipogenic and neurogenic differentiation potentials of stem cells from apical papilla by up-regulating SOX2 and OCT4. PMID: 28244619
SFRP2 is not only an agonist of Wnt pathway, but also a cancer promoting protein for lung cancer. PMID: 26323494
DKK1 (dickkopf-1) and SFRP2 (secreted frizzled-related protein 2) were the targets of miR-522 in hepatocellular carcinoma PMID: 26960688
the demethylation of SFRP2 gene appeared to inhibit nuclear retention of a key Wnt signaling factor, b-catenin, in osteosarcoma (OS) cell lines. Together, these data suggest that SFRP2 may function as an OS invasion suppressor by interfering with Wnt signaling, and the methylation of SFRP2 gene may promote pathogenesis of OS PMID: 26628297
SFRP2 hypermethylation is associated with colorectal cancer. PMID: 27221916
Silencing KDM2A, a histone demethylase and BCL6 co-repressor, de-repressed SFRP2 transcription by increasing histone H3K4 and H3K36 methylation at the SFRP2 promoter. PMID: 27074224
SFRP2 protein plays a role in the ultraviolet rays induced hyperpigmentary disorders. PMID: 26763443
age-related increases in sFRP2 augment both angiogenesis and metastasis of melanoma cells PMID: 27042933
Patients with Cytogenetically Normal Primary Acute Myeloid Leukemia and high sFRP2 expression had higher incidence of complete remission rate and better overall survival. PMID: 26517501
A combination of GATA5 and SFRP2 methylation could be promising as a marker for the detection and diagnosis of colorectal cancers and adenomas. PMID: 25759530
The distribution of Sfrp2 (and Sfrp1) in the eye is consistent with the idea that they modulate visual pathfinding and axon guidance. PMID: 25788689
These findings suggest that decreased SFRP2 expression is associated with intermediate/poor karyotypes in acute myeloid leukemia PMID: 25197341
The role of SFRP2, as a functional tumor suppressor in the development of oral squamous cell carcinoma, is mediated through inhibition of the Wnt signaling pathway. PMID: 25189527
Data suggest that overexpression of secreted frizzled-related protein 2 (sFRP2) gene in mesenchymal stem cells (hMSCs) might improve the therapeutic effectiveness of hMSC transplantation. PMID: 25245632
Aberrant methylation of APC gene was statistically significant associated with age over 50, DDK3 with male, SFRP4, WIF1, and WNT5a with increasing tumor stage SFRP4 and WIF1 with tumor differentiation and SFRP2 and SFRP5 with histological type PMID: 25107489
In hepatitis C virus infected liver tissues hypermethylation at promoter regions of key cancer related genes SFRP2 and DKK1, appears early at chronic hepatitis and liver cirrhosis stages, long before the appearance of hepatocellular carcinoma. PMID: 24947038
High SFRP2 gene methylation is associated with ovarian cancer infected with high risk human papillomavirus. PMID: 24761891
The risk size of SFRP2 hypermethylation from normal control to adenoma or polyp as well as from adenoma or polyp to colorectal cancer increased gradually PMID: 25053594
results show that sFRP-2 is a target gene of hypermethylation in esophageal Basaloid squamous cell carcinoma PMID: 24464051
SFRP2 appears to interact with Slug to affect the apoptosis of hypertrophic scar fibroblasts PMID: 23226515
show that SFRP2 hypermethylation is a common event in prostate cancer. SFRP2 methylation in combination with other epigenetic markers may be a useful biomarker of prostate cancer PMID: 22915211
Promoter hypermethylation of tumor suppressor SFRP2 is associated with prostate carcinoma. PMID: 22136354
Recombinant sFRP2 enhanced Wnt3a-dependent phosphorylation of LRP6 as well as both cytosolic beta-catenin levels and its nuclear translocation. sFRP2 enhanced the Wnt3a-mediated transcription of several genes including DKK1 and NKD1. PMID: 20723538
Data suggest that silencing of secreted frizzled-related protein 2 expression through promoter hypermethylation may be a factor in ESCC carcinogenesis through loss of its tumor-suppressive activity. PMID: 22363119
there is a loss of SFRP-2 expression from benign to malignant prostate glands and differential SFRP-2 expression among two possible subgroups of Gleason grade 5 tumours PMID: 22175903
Hypermethylation of SFRP2 gene is associated with advanced gastric cancer. PMID: 21409489
SFRP2 may play an important role in the development of earlobe keloid, especially at the keloid edge. PMID: 21174795
A study evaluating the pattern of SFRP2 methylation throughout the promoter during progressive tumorigenesis. PMID: 21709714
Among the extracellular regulators that suppress the Wnt pathway, secreted frizzled-related protein 2 (SFRP2), was up-regulated 4.3-fold in healthy smokers and 4.9-fold in COPD smokers. PMID: 21490961
serum SFRP2 methylation status represents a promising, non-invasive marker for colorectal carcinoma detection and staging PMID: 21463549
Combined effects of epigenetic alterations in SFRP2 and point mutations in K-ras protein play a role in development of mucinous type anal adenocarcinoma. PMID: 20686305
Promoter hypermethylation of SFRP2 is associated with Acute myeloid leukemia. PMID: 20795789
SFRP2 promoter methylation is aberrant in mesothelioma. PMID: 20596629
induces transient rise of intracellular Ca2+ via emptying of intracellular calcium stores in neutrophils PMID: 20602801
These results support sFRP2's role as an enhancer of Wnt3A/beta-catenin signaling, a result with biological impact for both normal development and diverse pathologies such as tumorigenesis. PMID: 20723538
This is the first report documenting that sFRP2 activates the canonical Wnt pathway and promotes cell growth by evoking diverse signaling cascades in renal cancer cells. PMID: 20501806

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Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

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Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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