Recombinant Human Sarcoplasmic/Endoplasmic Reticulum Calcium Atpase 2 (ATP2A2) Protein (His)

Name: Recombinant Human Sarcoplasmic/Endoplasmic Reticulum Calcium Atpase 2 (ATP2A2) Protein (His)
Brand: Beta LifeScience
SKU: BLC-03642P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Sarcoplasmic/Endoplasmic Reticulum Calcium Atpase 2 (ATP2A2) Protein (His) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P16615
Target Symbol	ATP2A2
Synonyms	AT2A2_HUMAN; Atp2a2; ATP2B; ATPase Ca++ transporting cardiac muscle slow twitch 2; Calcium pump 2; Calcium-transporting ATPase sarcoplasmic reticulum type; Calcium-transporting ATPase sarcoplasmic reticulum type slow twitch skeletal muscle isoform; Cardiac Ca2+ ATPase; DAR; DD; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; MGC45367; Sarcoplasmic/endoplasmic reticulum calcium ATPase 2; SERCA 2; SERCA2; serca2a; slow twitch skeletal muscle isoform; SR Ca(2+)-ATPase 2
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	VITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCRMFILDRVEGDTCSLNEFTITGSTYAPIGEVHKDDKPVNCHQYDGLVELATICALCNDSALDYNEAKGVYEKVGEATETALTCLVEKMNVFDTELKGLSKIERANACNSVIKQLMKKEFTLEFSRDRKSMSVYCTPNKPSRTSMSKMFVKGAPEGVIDRCTHIRVGSTKVPMTSGVKQKIMSVIREWGSGSDTLRCLALATHDNPLRREEMHLEDSANFIKYETNLTFVGCVGMLDPPRIEVASSVKLCRQAGIRVIMITGDNKGTAVAICRRIGIFGQDEDVTSKAFTGREFDELNPSAQRDACLNARCFARVEPSHKSKIVEFLQSFDEITAMTGDGVNDAPALKKAEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNM
Expression Range	314-756aa
Protein Length	Partial
Mol. Weight	52.3kDa
Research Area	Cardiovascular
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Involved in autophagy in response to starvation. Upon interaction with VMP1 and activation, controls ER-isolation membrane contacts for autophagosome formation. Also modulates ER contacts with lipid droplets, mitochondria and endosomes.; Involved in the regulation of the contraction/relaxation cycle. Acts as a regulator of TNFSF11-mediated Ca(2+) signaling pathways via its interaction with TMEM64 which is critical for the TNFSF11-induced CREB1 activation and mitochondrial ROS generation necessary for proper osteoclast generation. Association between TMEM64 and SERCA2 in the ER leads to cytosolic Ca(2+) spiking for activation of NFATC1 and production of mitochondrial ROS, thereby triggering Ca(2+) signaling cascades that promote osteoclast differentiation and activation.
Subcellular Location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Sarcoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families	Cation transport ATPase (P-type) (TC 3.A.3) family, Type IIA subfamily
Database References	HGNC: 812 OMIM: 101900 KEGG: hsa:488 STRING: 9606.ENSP00000440045 UniGene: Hs.506759
Associated Diseases	Acrokeratosis verruciformis (AKV); Darier disease (DD)
Tissue Specificity	Isoform 1 is widely expressed in smooth muscle and nonmuscle tissues such as in adult skin epidermis, with highest expression in liver, pancreas and lung, and intermediate expression in brain, kidney and placenta. Also expressed at lower levels in heart a

Gene Functions References

SGK3 a kinase transcriptionally regulated by estrogen receptor alpha (ERalpha) in breast cancer, sustains ERalpha signaling and drives the acquired aromatase inhibitors resistance by protecting against endoplasmic reticulum (EnR) stress-induced ERalpha downregulation and cell death through preserving SERCA2b function. PMID: 28174265
Darier disease (DD) is a rare autosomal dominant skin disorder due to mutations in the ATP2A2 gene. PMID: 29142187
SERCA2a gene transfer significantly improves left ventricle function and dimensions in doxorubicin-induced cardiomyopathy, suggesting LV-SERCA2a gene transfer an attractive treatment modality for doxorubicin-induced heart failure. PMID: 27203155
Studies indicate that Darier disease (DD) is caused by mutations in the ATP2A2 gene, whereas the ATP2C1 gene is associated with Hailey-Hailey disease (HHD). PMID: 28035777
Taken together, these results suggest that SERCA2 contributes to the migration of CCL21-activated Dendritic Cells as an important feature of the adaptive immune response and provide novel insights regarding the role of SERCA2 in Dendritic Cells functions. PMID: 27538371
Novel mutations in Darier disease and association to self-reported disease severity PMID: 29028823
VMP1 modulates SERCA2 activity to control endoplasmic reticulum contacts for autophagosome formation. PMID: 28890335
The left atrium / right atrium expression ratio was significantly increased in Atrial fibrillation for SERCA2 - gene related to calcium uptake and release, and located on the sarcoplasmic reticulum membrane. PMID: 27494721
Loss of SERCA2 impairs ER-to-Golgi transport of nascent DC. PMID: 28156030
We propose that the increased SERCA1a expression indicates the existence and location of compensating mechanisms in ischemic muscle. PMID: 28648117
Results show that ATP2A2 is variably expressed in astrocytoma tissues and its expression correlates with tumor grade. Its overexpression suppresses growth of astrocytoma cells. PMID: 28339043
study identifies a novel splice acceptor site mutation in the ATP2A2 gene, in a family showing Darier disease PMID: 27595213
Data suggest that mutations of the sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (ATP2A2) gene may lead to the occurrence of Darier disease (DD) in both familial and sporadic cases with DD. PMID: 27577212
Phospholamban and sarcolipin are membrane proteins that differentially regulate SERCA function. (Review) PMID: 26743715
High SERCA2 expression is associated with colorectal cancer. PMID: 26608678
Quantification of SERCA2 mRNA or protein expression levels revealed no differences in airway smooth muscle cells obtained from subjects with asthma compared to non-asthmatic controls. PMID: 25880173
Inhibition of SERCA2 activity by curcumin disrupts the Ca(2+) homeostasis and thereby promotes apoptosis in ovarian cancer cells. PMID: 26607901
The association between Darier disease and bipolar disorder is manifest also in the population, and data suggest that genetic variability within the ATP2A2 gene that causes Darier disease also confers susceptibility for bipolar disorder. PMID: 25213221
ATP2A2 mutation was found in patients diagnosed with Darier disease. PMID: 26051059
Two novel missense mutations, p.R603I and p.G749V, in the ATP2A2 gene in two families containing individuals diagnosed with Darier disease. PMID: 25872913
study identified two heterozygous mutations in exon 12 of the ATP2A2 gene in two different Darier disease families PMID: 24552170
Case Report: ATP2A2 missense mutation at one of the calcium-binding residues causing Darier's disease. PMID: 24979555
We report here two Chinese Han patients with sporadic Darier disease; mutation analysis of ATP2A2 supported the genetic diagnosis. PMID: 25283811
SPCA1 and sarco/endoplasmic reticulum Ca(2+) ATPase2 (SERCA2) encoded by ATP2A2 are two essential calcium pumps needed for Ca(2+) homeostasis maintenance in keratinocytes. PMID: 25256005
SERCA and PMCA pump activities are strongly affected by the localization of F508del-CFTR protein. PMID: 25661196
Evidence obtained from different diabetes models has suggested a role for advanced glycation end products formation, oxidative stress and increased O-GlcNAcylation in the lowered SERCA2 expression observed in diabetic cardiomyopathy PMID: 25270119
Gene therapy in heart failure. SERCA2a as a therapeutic target. PMID: 25327883
PDE3A is part of a SERCA2 signaling complex in cardiac myocytes. PMID: 25593322
ERK activation is sufficient to reduce SERCA2 mRNA. PMID: 25008120
A large Chinese family have a novel missense mutation in Darier disease. PMID: 23621824
Exposure of normal keratinocytes to the SERCA2 inhibitor thapsigargin recapitulated these abnormalities, supporting the role of loss of SERCA2 function in impaired desmosome and adherens junction formation. PMID: 24390139
a novel role of SERCA2b in facilitating the blockade of human liposarcoma differentiation PMID: 24508653
palmitoylated calnexin interacts with sarcoendoplasmic reticulum Ca(2+) transport ATPase 2b and this interaction determines endoplasmic reticulum Ca(2+) content and the regulation of endoplasmic reticulum-mitochondria Ca(2+) crosstalk. PMID: 23843619
I-1 and sarco/endoplasmic reticulum Ca2+ -ATPase synergistically induce the vascular smooth muscle cell contractile phenotype. PMID: 24249716
the protective effect of hepatic stimulator substance against endoplasmic reticulum stress may be associated with the removal of reactive oxygen species to restore the activity of the sarco-endoplasmic reticulum Ca(2+)-ATPase. PMID: 24284796
Novel ATP2A2 mutations in a large sample of individuals with Darier disease. PMID: 23356892
The results demonstrate that (*)NO-mediated activation of SERCA2b via S-glutathiolation of cysteine-674 is required for VEGF-induced EC Ca(2+) influx and migration, and establish redox regulation of SERCA2b as a key component in angiogenic signaling. PMID: 22472004
Downregulation of SERCA2a plays a critical role in modulating vascular and right ventricular phenotype associated with pulmonary arterial hypertension. PMID: 23804254
The gene encodes a calcium-ATPase type 2 in the sarco-/endoplasmic reticulum (SERCA2), which belongs to the large family of P-type cation pumps. PMID: 23337962
By introducing SERCA2 gene to an experimental heart failure model, cardiac functions and prognosis were improved and cardiac remodelling suppressed. SERCA2 is believed to be an important key to correct molecular network in heart failure. PMID: 23229631
The protein SERCA2 expression was decreased and 43 miRNAs were deregulated in infarcted myocardium compared to corresponding remote myocardium. PMID: 23066896
Shared histopathological features of acrokeratosis verruciformis of Hopf with Darier disease suggest that they are allelic disorders with variable expression of the same disease. Identical mutations in ATP2A2 in both diseases were not reported to date. PMID: 22814319
we report one novel heterozygous splice site mutation of ATP2A2 gene and one previously described nonsense mutation. PMID: 22909361
a higher Ca(2+) affinity of SERCA2b relative to other SERCA isoforms, not only on the cytosolic side, but also on the luminal side. PMID: 23024360
SERCA2 dysregulation is a pathogenic event in 22q11 deletion syndrome (22q11DS) and schizophrenia. PMID: 23055483
We report two novel mutations of the ATP2A2 gene in two Chinese families with Darier disease. PMID: 22329366
Review article highlights the regulatory mechanisms of cardiac contractility by way of the multimeric SERCA/phospholamban (PLN)-ensemble. PMID: 22679139
functional interactions of SERCA2b and Bcl-2 in the cell may be modulated by HSP70 and other chaperones and stress-regulated proteins. PMID: 22360692
Loss of SERCA2 expression is observed in human diabetes and occurs within the context of PPAR-gamma phosphorylation and CDK5 activation. PMID: 22240811
SERCA2-controlled Ca(2)+-dependent keratinocyte adhesion and differentiation is mediated via the sphingolipid pathway. PMID: 22277942

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