Recombinant Human Ribonuclease Uk114 (RIDA) Protein (GST)
Beta LifeScience
SKU/CAT #: BLC-10033P

Greater than 90% as determined by SDS-PAGE.
Recombinant Human Ribonuclease Uk114 (RIDA) Protein (GST)
Beta LifeScience
SKU/CAT #: BLC-10033P
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Description | Recombinant Human Ribonuclease Uk114 (RIDA) Protein (GST) is produced by our E.coli expression system. This is a full length protein. |
Purity | Greater than 90% as determined by SDS-PAGE. |
Uniprotkb | P52758 |
Target Symbol | RIDA |
Synonyms | 14.5 kDa translational inhibitor protein; 2-iminobutanoate/2-iminopropanoate deaminase; Heat responsive protein 12; Heat-responsive protein 12; Hrsp12; p14.5; Perchloric acid soluble protein; PSP; Reactive intermediate imine deaminase A homolog; Ribonuclease UK114; RIDA; Translational inhibitor p14.5; Translational inhibitor protein p14.5; UK114; UK114 antigen homolog; UK114_HUMAN |
Species | Homo sapiens (Human) |
Expression System | E.coli |
Tag | N-GST |
Target Protein Sequence | SSLIRRVISTAKAPGAIGPYSQAVLVDRTIYISGQIGMDPSSGQLVSGGVAEEAKQALKNMGEILKAAGCDFTNVVKTTVLLADINDFNTVNEIYKQYFKSNFPARAAYQVAALPKGSRIEIEAVAIQGPLTTASL |
Expression Range | 2-137AA |
Protein Length | Full Length of Mature Protein |
Mol. Weight | 41.4kDa |
Research Area | Epigenetics And Nuclear Signaling |
Form | Liquid or Lyophilized powder |
Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution | Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
Storage | 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Target Details
Target Function | Catalyzes the hydrolytic deamination of enamine/imine intermediates that form during the course of normal metabolism. May facilitate the release of ammonia from these potentially toxic reactive metabolites, reducing their impact on cellular components. It may act on enamine/imine intermediates formed by several types of pyridoxal-5'-phosphate-dependent dehydratases including L-threonine dehydratase.; Also promotes endoribonucleolytic cleavage of some transcripts by promoting recruitment of the ribonuclease P/MRP complex. Acts by bridging YTHDF2 and the ribonuclease P/MRP complex. RIDA/HRSP12 binds to N6-methyladenosine (m6A)-containing mRNAs containing a 5'-GGUUC-3' motif: cooperative binding of RIDA/HRSP12 and YTHDF2 to such transcripts lead to recruitment of the ribonuclease P/MRP complex and subsequent endoribonucleolytic cleavage. |
Subcellular Location | Cytoplasm. Nucleus. Peroxisome. Mitochondrion. |
Protein Families | RutC family |
Database References | |
Tissue Specificity | Expressed predominantly in liver and kidney. Lower levels in lung and brain. |
Gene Functions References
- x-ray crystallography study of p14.5 PMID: 14997576
- analysis of ligand binding sites of protein p14.5 PMID: 16198412