Recombinant Human Receptor-Type Tyrosine-Protein Phosphatase T (PTPRT) Protein (His)

Name: Recombinant Human Receptor-Type Tyrosine-Protein Phosphatase T (PTPRT) Protein (His)
Brand: Beta LifeScience
SKU: BLC-11063P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Receptor-Type Tyrosine-Protein Phosphatase T (PTPRT) Protein (His) is produced by our Baculovirus expression system. This is a protein fragment.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	O14522
Target Symbol	PTPRT
Synonyms	Protein tyrosine phosphatase receptor type T; PTPRT; PTPRT_HUMAN; R PTP T; R-PTP-T; Receptor protein tyrosine phosphatase; Receptor-type tyrosine-protein phosphatase rho; Receptor-type tyrosine-protein phosphatase T; RPTP-rho; RPTPmam4; RPTPrho
Species	Homo sapiens (Human)
Expression System	Baculovirus
Tag	N-6His
Target Protein Sequence	QSAAGGCSFDEHYSNCGYSVALGTNGFTWEQINTWEKPMLDQAVPTGSFMMVNSSGRASGQKAHLLLPTLKENDTHCIDFHYYFSSRDRSSPGALNVYVKVNGGPQGNPVWNVSGVVTEGWVKAELAISTFWPHFYQVIFESVSLKGHPGYIAVDEVRVLAHPCRKAPHFLRLQNVEVNVGQNATFQCIAGGKWSQHDKLWLQQWNGRDTALMVTRVVNHRRFSATVSVADTAQRSVSKYRCVIRSDGGSGVSNYAELIVKEPPTPIAPPELLAVGATYLWIKPNANSIIGDGPIILKEVEYRTTTGTWAETHIVDSPNYKLWHLDPDVEYEIRVLLTRPGEGGTGPPGPPLTTRTKCADPVHGPQNVEIVDIRARQLTLQWEPFGYAVTRCHSYNLTVQYQYVFNQQQYEAEEVIQTSSHYTLRGLRPFMTIRLRLLLSNPEGRMESEELVVQTEEDVPGAVPLESIQGGPFEEKIYIQWKPPNETNGVITLYEINYKAVGSLDPSADLSSQRGKVFKLRNETHHLFVGLYPGTTYSFTIKASTAKGFGPPVTTRIATKISAPSMPEYDTDTPLNETDTTITVMLKPAQSRGAPVSVYQLVVKEERLQKSRRAADIIECFSVPVSYRNASSLDSLHYFAAELKPANLPVTQPFTVGDNKTYNGYWNPPLSPLKSYSIYFQALSKANGETKINCVRLATKGASTQNSNTVEPEKQVDNTVKM
Expression Range	26-747aa
Protein Length	Partial
Mol. Weight	81.3
Research Area	Signal Transduction
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	May be involved in both signal transduction and cellular adhesion in the CNS.
Subcellular Location	Membrane; Single-pass type I membrane protein.
Protein Families	Protein-tyrosine phosphatase family, Receptor class 2B subfamily
Database References	HGNC: 9682 OMIM: 608712 KEGG: hsa:11122 STRING: 9606.ENSP00000362283 UniGene: PMID: 27244256 PTPRT promoter methylation is significantly associated with sensitivity to STAT3 inhibition in HNSCC cells, suggesting that PTPRT promoter methylation may serve as a predictive biomarker for responsiveness to STAT3 inhibitors in clinical development PMID: 25982282 Data reported evidence that rs2866943 polymorphism in PTPRT 3'-UTR was involved in the occurrence of esophageal squamous cell carcinoma by acting as a protective factor while rs6029959 acts as a risk factor. PMID: 25967969 Data show that hepatitis B virus X protein mutant HBxDelta127 enhances proliferation of hepatoma cells through up-regulating miR-215 targeting protein tyrosine phosphatase, receptor type T (PTPRT). PMID: 24434140 tumor-specific mutational events in the PTPRT gene can serve as direct drivers for tumor growth by inducing hyperactivation of STAT3, a potent oncogenic transcription factor and PTPRT substrate PMID: 24395800 The catalytic domain point mutants show a decreased thermal and thermodynamic stability and decreased activation energy relative to phosphatase activity, when compared to wild- type PMID: 22389709 [review] High-throughput mutational analysis identifies loss-of-function mutations in six PTPs in human colon cancers, providing critical cancer genetics evidence that PTPs can act as tumour suppressor genes. PMID: 21517784 Data show that paxillin is a direct substrate of PTPRT and that PTPRT specifically regulates paxillin phosphorylation at tyrosine residue 88(Y88). PMID: 20133777 alterations of the PTPRT-mediated signaling pathway by PTPRT phosphatase domain mutation may not play a critical role in the development of common human cancers PMID: 17223850 STAT3 is a substrate of receptor protein tyrosine phosphatase T PMID: 17360477 brain-specific PTPRT regulates synapse formation through interaction with cell adhesion molecules, and this function and the phosphatase activity are attenuated through tyrosine phosphorylation by the synaptic tyrosine kinase Fyn. PMID: 19816407

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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Recombinant Human Receptor-Type Tyrosine-Protein Phosphatase T (PTPRT) Protein (His)

Recombinant Human Receptor-Type Tyrosine-Protein Phosphatase T (PTPRT) Protein (His)

Product Overview

Target Details

FAQs