Recombinant Human R-Spondin 3/RSPO3 Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0049UL

Recombinant Human R-Spondin 3/RSPO3 Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0049UL
/
Size

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Product Overview

Product Name Recombinant Human R-Spondin 3/RSPO3 Protein
Target Symbol RSPO3
Alternative Names Roof Plate-specific Spondin 3, RSPO3, Thrombospondin Type I Domain Containing 2, Protein With TSP Type-1 Repeat, THSD2, PWTSR, CRISTIN1, HPWTSR, HRspo3, FLJ14440
Accession Number Q9BXY4
Expression System Mammalian cell line.
Molecular Weight 28.2 kDa (predicted)
Sequence Asn23-His272
Purity >98% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining
Activity Determined by the ability to promote proliferation of neuroblastoma cells.The ED50 for this effect is typically <40 ng/mL.
Formulation Lyophilized from sterile PBS with Trehalose, pH 7.4
Endotoxin Level <0.005 EU per 1 μg of the protein as determined by the LAL method
Shipping Shipped at ambient temperature.
Stability & Storage 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.
Reconstitution Reconstitute at 100 ug/mL in sterile PBS.
Target Function Roof plate-specific Spondin 3 (R-Spondin 3 or RSPO3) is a key regulator of Wnt signaling with diverse functions in during embryonic development, tissue homeostasis, and stem cell maintenance. In embryonic development, RSPO3 is involved in various processes, including limb bud development, skeletal patterning, and organogenesis. In adults, it plays a role in maintaining tissue homeostasis, particularly in stem cell niches where Wnt signaling is critical for the regulation of stem cell self-renewal and differentiation. RSPO3 enhances Wnt signaling by competing with Wnt antagonist DKK-1 for binding to Low-Density Lipoprotein Receptor-related Proteins LRP4, LRP5 or LRP6 and inhibiting its interaction with the co-receptor Kremen. This inhibition leads to the stabilization of the Frizzled/LRP receptor complex, which initiates downstream signaling events mediated by Wnt proteins. As the in vivo roles of RSPO3 have been elucidated in tissue patterning and stem cell maintenance, RSPO3 plays a more common role for in vitro cell culture and the growth and maintenance of 3-D organoid cultures.
Tissue Specificity R-Spondin 3 expression has been identified in limb buds, neural tube, Intestinal crypt epithelial cells, mammary glands, lung epithelium, liver bile ducts, basal layer of epidermis, hair follicles, ovaries and testes.
Cellular Localization Secreted protein
Involvement In Disease Dysregulation of R-Spondin 3 is associated with autosomal dominant polycystic kidney disease (ADPKD), brachydactyly type B1 (BDB1), Tetra-amelia syndrome, aplastic anemia and thrombocytopenia (TAAD), osteoarthritic cartilage, and numerous cancers (colorectal, gastric, breast, ovarian, liver and prostate).

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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