Recombinant Human R-Spondin 1/RSPO1 Protein, Active

Beta LifeScience SKU/CAT #: BEP-0044

Recombinant Human R-Spondin 1/RSPO1 Protein, Active

Beta LifeScience SKU/CAT #: BEP-0044
/
Size

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Product Overview

Product Name Recombinant Human R-Spondin 1/RSPO1 Protein
Target Symbol RSPO1
Alternative Names Roof Plate-specific Spondin 1, RSPO1, CRISTIN3, R-Spondin Homolog, HRspo1, RSPO, FLJ40906
Accession Number Q2MKA7
Expression System Mammalian cell line.
Molecular Weight 26.9 kDa (predicted)
Sequence Ser21-Ala263, with an N-terminal Met
Purity Determined by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining
Activity Determined by in-house activity assay
Formulation Lyophilized from sterile PBS with Trehalose, pH 7.4
Endotoxin Level Available Upon Request
Shipping Shipped at ambient temperature.
Stability & Storage 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.
Reconstitution Reconstitute at 100 ug/mL in sterile PBS.
Target Function Roof Plate-specific Spondin 1 (R-Spondin 1 or RSPO1) is a key regulator of Wnt signaling with diverse functions in during embryonic development, tissue homeostasis, and stem cell maintenance. Its precise role depends on the cellular context and interactions with other signaling pathways. RSPO1 enhances Wnt signaling by competing with Wnt antagonist DKK-1 for binding to LRP6 (Low-Density Lipoprotein Receptor-related Protein 6) and inhibiting its interaction with the co-receptor Kremen. This inhibition leads to the stabilization of the Frizzled/LRP6 receptor complex, which initiates downstream signaling events mediated by Wnt proteins. The role in tissue patterning is observed in expression of RSPO1 in the roof plate boundary during early development leading to involvement in organogenesis and gonadal development of both ovaries and testes. As the in vivo roles of RSPO1 have been elucidated in tissue patterning and stem cell maintenance, RSPO1 plays a more common role for in vitro cell culture and the growth and maintenance of 3-D organoid cultures.
Tissue Specificity Intestinal crypt epithelial cells, kidney epithelial cells, basal layer of epidermis, hair follicles, and prostate tissue.
Cellular Localization Secreted protein
Involvement In Disease Dysregulation of RSPO1 expression is implicated in various cancers due to enhanced Wnt signaling and the resultant cell proliferation and metastasis of tumors, as well as palmoplantar hyperkeratosis. Rare genetic mutations in the RSPO1 gene are linked to polydactyly, syndactyly and XX disorders of sex development with atypical gonadal development.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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