Recombinant Human Proteinase 3 Protein, Glycosylation Mutant

Name: Recombinant Human Proteinase 3 Protein, Glycosylation Mutant
Brand: Beta LifeScience
SKU: BAR-0134P
Availability: InStock

Collections: All products, High-quality recombinant proteins

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Description	A "Human Proteinase 3 (PR3) protein, Glycosylation Mutant" refers to a modified version of the PR3 protein where the normal process of glycosylation has been altered, typically by changing the asparagine residues that are sites for N-linked glycosylation. This modification can affect the protein's stability and activity, and is used to study the role of glycosylation in PR3's function, particularly in conditions like autoimmune vasculitis.
Source	HEK293 Cells
Form	Frozen
Buffer	Frozen in 50 mM MES, pH 4.5, with 700 mM NaCl
Purity	Greater than 95% as determined by SDS-PAGE.
Molecular Weight	29 kDa
Protein Determination	Extinction Coefficient (E) = 1.00 (0.1% at 280 nm, 1 cm pathway)
Storage/Handling	≤ –70 °C

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.