Recombinant Human Protein Wnt-4 (WNT4) Protein (His)

Name: Recombinant Human Protein Wnt-4 (WNT4) Protein (His)
Brand: Beta LifeScience
SKU: BLC-06559P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Human Protein Wnt-4 (WNT4) Protein (His) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P56705
Target Symbol	WNT4
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	C-6His
Target Protein Sequence	SNWLYLAKLSSVGSISEEETCEKLKGLIQRQVQMCKRNLEVMDSVRRGAQLAIEECQYQFRNRRWNCSTLDSLPVFGKVVTQGTREAAFVYAISSAGVAFAVTRACSSGELEKCGCDRTVHGVSPQGFQWSGCSDNIAYGVAFSQSFVDVRERSKGASSSRALMNLHNNEAGRKAILTHMRVECKCHGVSGSCEVKTCWRAVPPFRQVGHALKEKFDGATEVEPRRVGSSRALVPRNAQFKPHTDEDLVYLEPSPDFCEQDMRSGVLGTRGRTCNKTSKAIDGCELLCCGRGFHTAQVELAERCSCKFHWCCFVKCRQCQRLVELHTCR
Expression Range	23-351aa
Protein Length	Full Length of Mature Protein
Mol. Weight	43.6 kDa
Research Area	Signal Transduction
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Ligand for members of the frizzled family of seven transmembrane receptors. Plays an important role in the embryonic development of the urogenital tract and the lung. Required for normal mesenchyme to epithelium transition during embryonic kidney development. Required for the formation of early epithelial renal vesicles during kidney development. Required for normal formation of the Mullerian duct in females, and normal levels of oocytes in the ovaries. Required for normal down-regulation of 3 beta-hydroxysteroid dehydrogenase in the ovary. Required for normal lung development and for normal patterning of trachael cartilage rings.
Subcellular Location	Secreted, extracellular space, extracellular matrix.
Protein Families	Wnt family
Database References	HGNC: 12783 OMIM: 158330 KEGG: hsa:54361 STRING: 9606.ENSP00000290167 UniGene: PMID: 28272483 We found a genetic association between rs11031006 (FSHB) SNP and endometriosis. WNT4 and VEZT genes constitute the most consistently associated genes with endometriosis. In the present study, an association of rs7521902 (WNT4) and rs10859871 (VEZT) was confirmed in women with endometriosis at the genotypic but not the allelic level. PMID: 28901453 urinary Wnt4 could be a potential noninvasive biomarker for the early detection of tubular injury. PMID: 27600466 were not able to replicate or further verify the genetic association of polymorphisms in WNT4 and WNT5B with bone mineral density PMID: 28078366 These findings demonstrate that autocrine human growth hormone regulates WNT4 expression and that WNT4 is a potential therapeutic target in human breast cancer. PMID: 27323961 WNT4 encodes wingless-type MMTV integration site family member 4. WNT4 mutations have been found in women with mullerian duct abnormalities, primary amenorrhea, and hyperandrogenism and common variants in WNT4, which are in high linkage disequilibrium with our index SNPs, are associated with endometriosis, ovarian cancer,and bone mineral density of WNT4, and the T allele generates a strong ESR1-binding site. PMID: 28877031 Our MRKH families included 43 quads, 26 trios, and 30 duos. Of our MRKH probands, 87/147 (59%) had MRKH type 1 and 60/147 (41%) had type 2 with additional anomalies. CONCLUSION(S): Although the prevalence of WNT4, HNF1B, and LHX1 point mutations is low in people with MRKH, the prevalence of CNVs was approximately 19%. PMID: 28600106 WNT4 drives a novel signaling pathway in ILC cells, with a critical role in estrogen-induced growth that may also mediate endocrine resistance. WNT4 signaling may represent a novel target to modulate endocrine response specifically for patients with ILC. PMID: 27650553 The etiology of MRKH syndrome is still largely unknown, probably because of its intrinsic heterogeneity. Several candidate causative genes have been investigated, but to date only WNT4 has been associated with MRKH with hyperandrogenism. This review summarizes and discusses the clinical features and details progress to date in understanding the genetics of MRKH syndrome. PMID: 27716927 We highlight the cooperation of WNT4, RSPO1 and FOXL2 within a regulatory network and the need for further research to better understand their role in defining and maintaining ovarian identity. PMID: 27604691 The WNT4 expression level in eutopic endometrium was significantly reduced compared with that in normal endometrium of the control group. PMID: 26363035 Results suggest that the Wnt4 gene encodes signals that are important for various aspects of female reproductive tract development. PMID: 26721931 Studied the roles of WNT4 and WNT5A in human decidulization and their relationship with preeclampsia. PMID: 26848991 Polymorphisms on WNT4 gene might be involved in the pathogenesis of endometriosis in the infertile women. PMID: 26139156 the expression of WNT4, a Wnt ligand, and three targets of Wnt-ss-catenin transcription activation, namely, MMP7, cyclinD1 (CD1) and c-MYC in 141 penile tissue cores from 101 unique samples, were investigated. PMID: 25901368 The results demonstrated that WNT4 rs2235529 is associated with endometriosis in Chinese Han women, which may result in aberrant expression of WNT4, leading to the pathogenesis of endometriosis. PMID: 25682310 Wnt4 might be a key gene during parathyroid hormone-induced epithelial mesenchymal transformation of proximal kidney tubule cells. PMID: 25337242 The 5'-flanking region of the Wnt4 gene is responsive to Pax8. Pax8 modulates the expression of Wnt4 in thyroid cells. PMID: 25270402 Our results suggest that the rs10965235 SNP in the CDKN2B-AS gene and the rs16826658 SNP near the WNT4 gene were significantly associated with endometriosis in this Korean population. PMID: 25154675 We report an adolescent girl with the cardinal features of MURCS association, obesity, and clinical findings of hyperandrogenism who did not show any exonic mutation of the WNT4 gene PMID: 24356390 These data indicate that Wnt4 signaling is deregulated in most pituitary adenomas and its excessive activation may inhibit pituitary tumor invasion. PMID: 24200887 State anxiety assessed two months before the examination was positively and negatively correlated with miR-16 and its target WNT4 mRNA levels, respectively. PMID: 24130753 The WNT4 ligand plays a role in regulating the cell growth of leukemia-derived cells by arresting cells in the G1 cell cycle phase. PMID: 24274766 Analysis of bronchial biopsy samples shows a very strong correlation between Wnt4 and interleukin (IL)8 gene expression, suggesting a role for Wnt4 in chronic lung inflammation. PMID: 23463699 heterozygous WNT4 variants are likely to play a causative role in renal hypodysplasia. PMID: 23520208 We confirm WNT4, CDKN2BAS and FN1 as the first identified common loci for endometriosis. PMID: 23142796 Recent developments have demonstrated that ovarian development is an active process (rather than a default process); ovarian development/function requires expression of WNT4, RSPO1, and FOXL2. [REVIEW] PMID: 23044875 Studies uncovered a linear pathway involving BMP2, WNT4/beta-catenin, and Forkhead box protein O1 that operates in human endometrium to critically control decidualization. PMID: 23142810 Mutations in the WNT4 gene is not associated with premature ovarian failure. PMID: 22951804 Mutations in WNT4 are not responsible for Mullerian duct abnormalities in Chinese women. PMID: 22503279 Wnt/beta-catenin pathway forms a negative feedback loop during TGF-beta1 induced human normal skin fibroblast-to-myofibroblast transition PMID: 22041457 Possible role of WNT4 in Han Chinese women with premature ovarian failure. PMID: 21624127 Report a new WNT4 gene mutation in atypical Mayer-Rokitansky-Kuster-Hauser syndrome. PMID: 21377155 These observations highlight the distinct roles of WNT11 and WNT4 during the early stages of retinoic acid-induced neuronal differentiation. PMID: 21280163 Wnt4 is specifically involved in joint development. PMID: 20709709 Findings provide the first convincing line of evidence that EAF and Wnt4 form an auto-regulatory negative feedback loop in vivo. PMID: 20161747 at least 1 cause of Mayer-Rokitansky-Kuster-Hauser (MRKH) syndrome is a loss of function mutation of the Wnt4 (wingless-type MMTV integration site family member 4) gene. PMID: 20060343 The age-related down-regulation of Wnt4 (and subsequently FoxN1), and the prominent increase in LAP2alpha expression, was investigated in thymic epithelial cells. PMID: 20502698 The current results support a view that WNT4 may have a role in oocyte selection and follicle formation and maturation in human ovaries. PMID: 19962424 Results demonstrated significant up-regulation of WNT-3, WNT-4, WNT-5B, WNT-7B, WNT-9A, WNT-10A, and WNT-16B in patients with CLL compared to normal subjects. PMID: 19863181 Wnt 4a gene encodes a growth factor that participates in the formation of the kidney, adrenal, mammary gland, pituitary, and female reproductive tract. Wnt4 is required for the initial steps of the formation of Mullerian ducts. PMID: 19849868 WNT-4 protein is expressed in the developing nephron during morphogenetic period of the kidney. PMID: 12768078 Down-regulation of Wnt-4 and up-regulation of Wnt-5a are possible markers of the malignant phenotype of human squamous cell carcinoma. PMID: 12841867 overexpression disrupts normal testicular vasculature and inhibits testosterone synthesis by repressing steroidogenic factor 1/beta-catenin synergy PMID: 12949260 matrilysin may have a role in renal tubular injury and progression of tubulointerstitial fibrosis, and Wnt4 may regulate matrilysin expression in the kidney PMID: 15149334 Their different spatial expression patterns suggest that Wnt4 and Wnt5a proteins are not functionally linked to type II collagen and type X collagen synthesis in in vitro chondrogenic models of mesenchyme stem cells PMID: 15389636 sequencing of all five exons of WNT-4 demonstrated no mutant alleles in any of 8 46,XX true hermaphrodites. The possibility of the existence of causative mutations in the untranslated regions of WNT-4, or within introns cannot be ruled out. PMID: 15589122 It is thus likely that both RE1 and RE2 are necessary in rendering p63/p73-specific activation of the WNT4 promoter. PMID: 16343436 Bone morphogenetic protein-2 modulates Wnt and frizzled expression and enhances the canonical pathway of Wnt signaling in normal keratinocytes PMID: 16442268 These data show that DKK3 and WNT4 have multiple actions on steroidogenesis in adrenocortical cells, including effects on overall steroidogenesis (aldosterone and cortisol biosynthesis) and distinct effects on steroidogenic enzyme mRNA levels. PMID: 16981135

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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