Recombinant Human Protein Chibby Homolog 1 (CBY1) Protein (GST)

Name: Recombinant Human Protein Chibby Homolog 1 (CBY1) Protein (GST)
Brand: Beta LifeScience
SKU: BLC-08640P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Protein Chibby Homolog 1 (CBY1) Protein (GST) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	Q9Y3M2
Target Symbol	CBY1
Synonyms	ARPP-binding protein; C22orf2; CBY; Cby1; CBY1_HUMAN; Cytosolic leucine rich protein ; Cytosolic leucine-rich protein; Golgi and endoplasmic reticulum-associated 1; HRIHFB2025; PIGEA 14; PIGEA-14; PKD2 interactor; PKD2 interactor, Golgi and endoplasmic reticulum-associated 1; Protein Chibby; Protein chibby homolog 1
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-GST
Target Protein Sequence	MPFFGNTFSPKKTPPRKSASLSNLHSLDRSTREVELGLEYGSPTMNLAGQSLKFENGQWIAETGVSGGVDRREVQRLRRRNQQLEEENNLLRLKVDILLDMLSESTAESHLMEKELDELRISRKRK
Expression Range	1-126aa
Protein Length	Full Length
Mol. Weight	41.5kDa
Research Area	Signal Transduction
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Inhibits the Wnt/Wingless pathway by binding to CTNNB1/beta-catenin and inhibiting beta-catenin-mediated transcriptional activation through competition with TCF/LEF transcription factors. Has also been shown to play a role in regulating the intracellular trafficking of polycystin-2/PKD2 and possibly of other intracellular proteins. Promotes adipocyte and cardiomyocyte differentiation.
Subcellular Location	Nucleus speckle. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Golgi apparatus. Golgi apparatus, trans-Golgi network.
Protein Families	Chibby family
Database References	HGNC: 1307 OMIM: 607757 KEGG: hsa:25776 STRING: 9606.ENSP00000216029 UniGene: PMID: 27528616 chronic Cby1 knockdown in colon cancer cells may counteract tumor progression by promoting the mesenchymal-to-epithelial transition process. PMID: 28107095 Cby's C-terminal domain alone binds to TC-1 with significantly greater affinity compared to full-length Cby, implying that target binding of the coiled-coil domain is affected by the flanking disordered regions. PMID: 27082063 CBY1 downregulation in CML comes from reduced protein stability when bound to 14-3-3sigma adapter protein. Dissociation raises CBY1 protein levels by enhancing its stability. The ubiquitin proteasome system reduces 14-3-3sigma-bound CBY1 stability by SUMOylation. PMID: 26147002 Study showed that expression of Cby protein was strongly downregulated in laryngeal squamous cell carcinoma (LSCC) tumor tissues in comparison to normal laryngeal mucosa samples. PMID: 25175341 the impact of BCR-ABL1 on Chibby1, a beta catenin antagonist involved in cell differentiation and transformation PMID: 24339928 Stable expression of CBY1 drives beta catenin cytoplasmatic translocation and impairs beta catenin signaling in BCR-ABL + cells. PMID: 23707389 Results from the association analysis suggest that common variation in CBY is not a cause for obesity in the Belgian population. PMID: 23645032 C-terminal polycystin-2 influences the interaction with PIGEA14 PMID: 23838289 Cby plays an important role in organization of both primary and motile cilia in collaboration with Cnx. PMID: 22911743 The N-terminal portion of the CBY1 is unstructured in solution, but the C-terminal half forms a coiled-coil structure. PMID: 21182262 Chibby and clusterin were co-immunoprecipitated with NBPF1. PMID: 20096688 These findings unravel the molecular basis through which a combinatorial action of Cby and 14-3-3 proteins controls the dynamic nuclear-cytoplasmic trafficking of beta-catenin. PMID: 19940019 Chibby inhibits beta-catenin-mediated transcriptional activation by competing with Lef-1 to bind to beta-catenin PMID: 12712206 PIGEA-14 plays an important role in regulating the intracellular location of polycystin-2 PMID: 15194699 As no somatic mutation was detected in C22orf2 in 36 colorectal tumour DNA, our results do not support the implication of Chibby as a tumour suppressor in colorectal carcinogenesis. PMID: 15245581 Chibby is not likely to promote colorectal carcinoma tumor development or progression. PMID: 16570344 CBY promotes adipocyte differentiation through inhibition of beta catenin signaling. PMID: 17403895 intrinsically disordered TC-1 interacts with Cby via its transient helical structure PMID: 17905836 14-3-3epsilon and 14-3-3zeta are identified as Cby-binding partners. PMID: 18573912 genes including CHIBBY involved in pediatric ependymomas. PMID: 18663750 Alanine substitutions of two or more of four critical leucine residues within the C-terminal heptad repeats eliminate the Cby-Cby interaction. PMID: 19435523

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.