Human PSAP (Prosaposin) - Recombinant Protein
Beta LifeScience
SKU/CAT #: BLT-07465P
SDS-PAGE analysis of Human PSAP (Prosaposin) - Recombinant Protein, CAT# BLT-07465P, showing >90% purity under 15% SDS-PAGE (Reduced)
Human PSAP (Prosaposin) - Recombinant Protein
Beta LifeScience
SKU/CAT #: BLT-07465P
Regular price
$69500
$695.00
Sale price$55600
$556.00Save $139
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Quantity Pricing
| Pack Size | Price (USD) |
|---|---|
| 500 µg | $1,206.00 Original price: |
| 1 mg | $2,284.00 Original price: |
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Product Overview
| Product Name | Recombinant Human Prosaposin (PSAP) Protein |
| Product Overview | This recombinant human Prosaposin (PSAP) protein includes amino acids 17-524aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBSprior to lyophilization. |
| Target Uniprot Id | P07602 |
| Recommended Name | Prosaposin |
| Gene Name | PSAP |
| Synonyms | SAP1; GLBA; CSAct; Dispersin; Pro-Saposin; Proactivator polypeptide; Glucosylceramidase activator; S |
| Species | Human |
| Predicted Molecular Mass | 60 kDa |
| Expression System | E.coli |
| Expression Range | 17-524aa |
| Tag | N-6His |
| Purity | >90% |
| Formulation | Lyophilized |
| Buffer | PBS |
| Storage Condition | 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction | Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications | Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area | Metabolism, Signal Transduction |
| Target Function | Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate.; Saposin-B stimulates the hydrolysis of galacto-cerebroside sulfate by arylsulfatase A (EC 3.1.6.8), GM1 gangliosides by beta-galactosidase (EC 3.2.1.23) and globotriaosylceramide by alpha-galactosidase A (EC 3.2.1.22). Saposin-B forms a solubilizing complex with the substrates of the sphingolipid hydrolases.; Saposin-D is a specific sphingomyelin phosphodiesterase activator (EC 3.1.4.12).; Behaves as a myelinotrophic and neurotrophic factor, these effects are mediated by its G-protein-coupled receptors, GPR37 and GPR37L1, undergoing ligand-mediated internalization followed by ERK phosphorylation signaling.; Saposins are specific low-molecular mass non-enzymic proteins, they participate in the lysosomal degradation of sphingolipids, which takes place by the sequential action of specific hydrolases. |
| Subcellular Location | Lysosome.; [Prosaposin]: Secreted. |
| Associated Diseases | Combined saposin deficiency (CSAPD); Leukodystrophy metachromatic due to saposin-B deficiency (MLD-SAPB); Gaucher disease, atypical, due to saposin C deficiency (AGD); Krabbe disease, atypical, due to saposin A deficiency (AKRD) |
