Recombinant Human Progonadoliberin-2 (GNRH2) Protein (His-SUMO)
Beta LifeScience
SKU/CAT #: BLC-09943P

Greater than 90% as determined by SDS-PAGE.
Recombinant Human Progonadoliberin-2 (GNRH2) Protein (His-SUMO)
Beta LifeScience
SKU/CAT #: BLC-09943P
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Description | Recombinant Human Progonadoliberin-2 (GNRH2) Protein (His-SUMO) is produced by our E.coli expression system. This is a full length protein. |
Purity | Greater than 90% as determined by SDS-PAGE. |
Uniprotkb | O43555 |
Target Symbol | GNRH2 |
Synonyms | GnRH II; GnRH-associated peptide 2; GnRH-associated peptide II; gnrh2; GON2_HUMAN; Gonadoliberin II; Gonadotropin-releasing hormone 2; Gonadotropin-releasing hormone II; LH-RH II; Luliberin II; Luteinizing hormone-releasing hormone II; Progonadoliberin 2; Progonadoliberin II |
Species | Homo sapiens (Human) |
Expression System | E.coli |
Tag | N-6His-SUMO |
Target Protein Sequence | QHWSHGWYPGGKRALSSAQDPQNALRPPGRALDTAAGSPVQTAHGLPSDALAPLDDSMPWEGRTTAQWSLHRKRHLARTLLTAAREPRPAPPSSNKV |
Expression Range | 24-120aa |
Protein Length | Full Length of Mature Protein |
Mol. Weight | 26.5kDa |
Research Area | Signal Transduction |
Form | Liquid or Lyophilized powder |
Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution | Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
Storage | 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Target Details
Target Function | Stimulates the secretion of gonadotropins; it stimulates the secretion of both luteinizing and follicle-stimulating hormones. |
Subcellular Location | Secreted. |
Protein Families | GnRH family |
Database References | |
Tissue Specificity | Midbrain; expressed at significantly higher levels outside the brain (up to 30-fold), particularly in the kidney, bone marrow and prostate. |
Gene Functions References
- GnRHII expression correlated significantly with poor prognosis in breast cancer patients. PMID: 23287110
- results suggest GnRH-II-induced laminin receptor precursor expression increases 67-kDa nonintegrin laminin receptor, which appears to interact with laminin in the extracellular matrix to promote MMP-2 expression and enhance ovarian cancer cell invasion PMID: 21193558
- concluded that GnRHII stimulates PI3K/Akt pathway, and the phosphorylation of glycogen synthase kinase3beta, thereby enhancing the beta-catenin-dependent up-regulation of MT1-MMP production which contributes to ovarian cancer metastasis PMID: 21239435
- These preliminary data suggest that local GnRH-II may participate in the regulation of ovarian tumor growth in post-menopausal women. PMID: 20018426
- a novel mechanism in stimulating basal human GnRH-II gene transcription mediated by cooperative actions of multiple regulatory elements within the untranslated first exon of the gene. PMID: 12663744
- GnRH-II has been identified, together with a 'putative' type II GnRH receptor, both in the central nervous system and in peripheral structures, such as tissues of the reproductive tract (both normal and tumoral). PMID: 14726258
- GnRH-II did not affect proliferation of normal B-cells alone, and did not alter the proliferative response to IL-2. PMID: 15578334
- Expression of GnRH2 protein and receptor (GnRHR2) is confirmed in both leiomyoma and patient-matched myometrium. PMID: 17296196
- these data demonstrate the differential roles of NF-kappaB p65 and RARalpha/RXRalpha, interacting with the same sequence in the promoter of the human GnRH II gene to influence gene expression in a cell-specific manner. PMID: 17451432
- GnRH II is widely expressed in prostate cancer and is an AR-regulated gene. PMID: 17914092
- p-CREB, C/EBPbeta, and CBP are recruited to the CRE of the GnRH-II promoter in a temporarily defined manner to enhance its transcription in JEG-3 and OVCAR-3 cells in response to cAMP PMID: 18599546
- These results provide evidence that EGF is an upstream regulator of the autocrine actions of GnRH-II on the invasive properties of ovarian cancer cells. PMID: 19608641