Recombinant Human Progesterone-Induced-Blocking Factor 1 (PIBF1) Protein (His&Myc)

Name: Recombinant Human Progesterone-Induced-Blocking Factor 1 (PIBF1) Protein (His&Myc)
Brand: Beta LifeScience
SKU: BLC-04803P
Availability: InStock

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of this product could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) PIBF1.

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Product Overview

Description	Recombinant Human Progesterone-Induced-Blocking Factor 1 (PIBF1) Protein (His&Myc) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	Q8WXW3
Target Symbol	PIBF1
Synonyms	PIBF1; C13orf24; PIBF; Progesterone-induced-blocking factor 1; Centrosomal protein of 90 kDa; CEP90
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-10His&C-Myc
Target Protein Sequence	MSRKISKESKKVNISSSLESEDISLETTVPTDDISSSEEREGKVRITRQLIERKELLHNIQLLKIELSQKTMMIDNLKVDYLTKIEELEEKLNDALHQKQLLTLRLDNQLAFQQKDASKYQELMKQEMETILLRQKQLEETNLQLREKAGDVRRNLRDFELTEEQYIKLKAFPEDQLSIPEYVSVRFYELVNPLRKEICELQVKKNILAEELSTNKNQLKQLTETYEEDRKNYSEVQIRCQRLALELADTKQLIQQGDYRQENYDKVKSERDALEQEVIELRRKHEILEASHMIQTKERSELSKEVVTLEQTVTLLQKDKEYLNRQNMELSVRCAHEEDRLERLQAQLEESKKAREEMYEKYVASRDHYKTEYENKLHDELEQIRLKTNQEIDQLRNASREMYERENRNLREARDNAVAEKERAVMAEKDALEKHDQLLDRYRELQLSTESKVTEFLHQSKLKSFESERVQLLQEETARNLTQCQLECEKYQKKLEVLTKEFYSLQASSEKRITELQAQNSEHQARLDIYEKLEKELDEIIMQTAEIENEDEAERVLFSYGYGANVPTTAKRRLKQSVHLARRVLQLEKQNSLILKDLEHRKDQVTQLSQELDRANSLLNQTQQPYRYLIESVRQRDSKIDSLTESIAQLEKDVSNLNKEKSALLQTKNQMALDLEQLLNHREELAAMKQILVKMHSKHSENSLLLTKTEPKHVTENQKSKTLNVPKEHEDNIFTPKPTLFTKKEAPEWSKKQKMKT
Expression Range	1-757aa
Protein Length	Full Length
Mol. Weight	97.2 kDa
Research Area	Cell Biology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Plays a role in ciliogenesis.; Pericentriolar protein required to maintain mitotic spindle pole integrity. Required for the centrosomal accumulation of PCM1 and the recruitment of centriolar satellite proteins such as BBS4. Via association with PCM1 may be involved in primary cilia formation. Required for CEP63 centrosomal localization and its interaction with WDR62. Together with CEP63 promotes centriole duplication. Promotes the centrosomal localization of CDK2.; The secreted form is a mediator of progesterone that by acting on the phospholipase A2 enzyme interferes with arachidonic acid metabolism, induces a Th2 biased immune response, and by controlling decidual naturakl killer cells (NK) activity exerts an anti-abortive effect. Increases the production of Th2-type cytokines by signaling via the JAK/STAT pathway. Activates STAT6 and inhibits STAT4 phosphorylation. Signaling via a not identified receptor seems to implicate IL4R and a GPI-anchored protein.
Subcellular Location	Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm. Secreted.; [Isoform 1]: Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite. Secreted.; [Isoform 4]: Secreted.
Database References	HGNC: 23352 OMIM: 607532 KEGG: hsa:10464 STRING: 9606.ENSP00000317144 UniGene: PMID: 29962287 blood and placental tissue level expression of PIBF is concordant and correlates with both progesterone receptor expression status as well as final pregnancy outcome PMID: 27728856 this study shows that preterm birth may be predictable at 24-28 gestational week by lower than normal pregnancy PIBF values PMID: 27479613 B cells are a significant producer of PIBF1 in human choriodecidua in late gestation. Preterm labor was associated with a lower level of active PIBF1 in late gestation choriodecidua. PMID: 27918564 PIBF plays a role in both normal pregnancy and tumor biology. PMID: 20367622 PIBF promotes proliferation, migration, and invasion of human glioblastoma cells. PMID: 28168193 These data show that exposure to an allogeneic stimulus is not needed to cause a marked rise in PIBF, merely progesterone alone is sufficient. PMID: 26634256 Maternal serum progesterone-induced blocking factor (PIBF) in the prediction of preterm birth. PMID: 25818991 progesterone-induced blocking factor is intracellularly expressed by the cultured cells from Glioblastoma PMID: 24474429 Progesterone-induced blocking factor is hormonally regulated in human astrocytoma cells, and increases their growth through the IL-4R/JAK1/STAT6 pathway PMID: 25218441 decreased kisspeptin and PIBF expressions in trophoblasts and deciduas are associated with recurrent spontaneous abortion PMID: 24225150 reduced expression during late pregnancy and resorption PMID: 24325791 Data indicate that silencing of progesterone-induced blocking factor (PIBF) increased invasiveness as well as MMP-2,-9 secretion of HTR8/SVneo trophoblast, and decreased those of HT-1080 cells. PMID: 23807209 CEP90 physically interacts with PCM-1 at centriolar satellites, and this interaction is essential for centrosomal accumulation of the centriolar satellites and eventually for primary cilia formation. PMID: 23110211 Suggest that urinary PIBF levels may predict graft rejection in transplant recipients. PMID: 22172828 increased serum levels after dydrogesterone supplementation in women with threatened preterm delivery; positive correlation with the length of gestation PMID: 22032897 CEP90 was characterized as a component of pericentriolar satellites. PMID: 21224392 PIBF affects the expression of leptin and its receptor, and PIBF expression is inversely related to trophoblast invasiveness. PMID: 21632119 promotes secretion of type 2 cytkines by activated lymphocytes from pregnant woman PMID: 19371956 The 48-kDa N-terminal part of PIBF is biologically active, and the part of the molecule responsible for modulating natural killer cell activity is encoded by exons 2-4. PMID: 14634107 PIBF production is a characteristic feature of normal pregnancy, and determination of PIBF concentration in urine might be of use for the diagnosis of threatened premature pregnancy termination. PMID: 15269099 progesterone induced blocking factor overexpression is associated with breast neoplasms PMID: 15305375 PIBF phosphorylates PKC via binding to the IL-4R, without affecting intracellular Ca(++). These findings explain the mechanism by which PIBF supports a Th2 dominant cytokine pattern. PMID: 16433831 PIBF concentrations in urine and serum of patients with threatened abortion were significantly lower than in healthy pregnant women. PMID: 19290853

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.