Recombinant Human Pro-Neuregulin-3, Membrane-Bound Isoform (NRG3) Protein (His-SUMO)

Name: Recombinant Human Pro-Neuregulin-3, Membrane-Bound Isoform (NRG3) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-04172P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of this product could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) NRG3.

Collections: High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Pro-Neuregulin-3, Membrane-Bound Isoform (NRG3) Protein (His-SUMO) is produced by our E.coli expression system. This is a extracellular protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P56975
Target Symbol	NRG3
Synonyms	HRG3; Neuregulin 3; Neuregulin 3 like polypeptide; Neuregulin-3; Neuregulin3; NRG-3; Nrg3; NRG3_HUMAN; Pro neuregulin 3; pro NRG3; Pro-NRG3
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	MSEGAAAASPPGAASAAAASAEEGTAAAAAAAAAGGGPDGGGEGAAEPPRELRCSDCIVWNRQQTWLCVVPLFIGFIGLGLSLMLLKWIVVGSVKEYVPTDLVDSKGMGQDPFFLSKPSSFPKAMETTTTTTSTTSPATPSAGGAASSRTPNRISTRLTTITRAPTRFPGHRVPIRASPRSTTARNTAAPATVPSTTAPFFSSSTLGSRPPVPGTPSTQAMPSWPTAAYATSSYLHDSTPSWTLSPFQDAASSSSSSSSSATTTTPETSTSPKFHTTTYSTERSEHFKPCRDKDLAYCLNDGECFVIETLTGSHKHCRCKEGYQGVRCDQFLPKTDSILSDPTDHLGIEFMESEEVYQRQ
Expression Range	1-360aa
Protein Length	Extracellular Domain
Mol. Weight	53.8kDa
Research Area	Cancer
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Direct ligand for the ERBB4 tyrosine kinase receptor. Binding results in ligand-stimulated tyrosine phosphorylation and activation of the receptor. Does not bind to the EGF receptor, ERBB2 or ERBB3 receptors. May be a survival factor for oligodendrocytes.
Subcellular Location	[Pro-neuregulin-3, membrane-bound isoform]: Cell membrane; Single-pass type I membrane protein.; [Neuregulin-3]: Secreted.; [Isoform 3]: Cell membrane; Single-pass type I membrane protein. Note=Isoform 3 is also proteolytically released as a soluble form.
Protein Families	Neuregulin family
Database References	HGNC: 7999 OMIM: 605533 KEGG: hsa:10718 STRING: 9606.ENSP00000361214 UniGene: Hs.125119
Tissue Specificity	Highly expressed in most regions of the brain with the exception of corpus callosum. Expressed at lower level in testis. Not detected in heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, col

Gene Functions References

No genetic correlation was found between Hirschsprung disease and the three SNPs at NRG3 (rs10748842, rs10883866 and rs6584400) PMID: 28256518
Data show that Williams-Beuren syndrome transcription factor (WSTF) release was mediated by neuregulin-3 (NRG3) following KRASG12V expression in intestinal epithelial cells. PMID: 27449290
These observations encourage investigation of the neurobiology of NRG3 isoforms and highlight inhibition of NRG3 signaling as a potential target for psychiatric treatment development. PMID: 27771971
common variants of GABRG2, RELN and NRG3 and the GABRG2-RELN-PTCH1 interaction networks might confer altered susceptibility to Hirschsprung disease. PMID: 27889765
these data suggest that Nrg3/ErbB4 signaling may be an important factor in nicotine dependence. PMID: 23999525
This study demonstrated that genetic variants in the NRG3 gene play a role in alzheimer disease and revealed that SNPs in the NRG3 genes were more strongly associated with onset age of Alzherimer disease. PMID: 24061483
An association between rs10748842 genotype in neuregulin 3 does not fit a simple inefficiency model of risk association in dorsolateral prefrontal cortex physiology. PMID: 24431462
NRG3 may be a susceptibility gene for Hirschsprung's disease in a Chinese population. PMID: 23315268
Neuregulin 3 rs6584400 genetic carriers are associated with psychotic symptoms and attention performance in schizophrenia. PMID: 22831755
no association of SNPs or haplotypes with risk of schizophrenia in Han Chinese population PMID: 22981155
Our results suggest a role for NRG3 in HSCR etiology and provide insights into the relative contribution of structural variants in both syndromic and non-syndromic HSCR. PMID: 22589734
NRG3 polymorphisms and haplotypes were similar between schizophrenia patients and healthy controls of Korean ethnicity PMID: 21762460
Data show that rs1649942, which is located in the intron region of NM_001165973.1. (NRG3 neuregulin 3), was associated with poorer overall survival in women with optimally debulked tumors. PMID: 21705454
Results suggest that NRG3 may be modulating early attentional processes for perceptual sensitivity and vigilance, with opposite effects in affected individuals and healthy controls. PMID: 20548296
Data show that NRG3 is a schizophrenia susceptibility gene, provide quantitative insight into NRG3 transcription traits in the brain, and reveal a probable mechanistic basis for disease association. PMID: 20713722
The NRG3 protein is a new post-translationally regulated isoform of neuregulin 3 expressed in the developing human central nervous system with a role in oligodendrocyte survival. PMID: 16478787
In transgenic female mice, Nrg3 has a role in the initiation of mammary placodes along the body axis; alterations in morphogenesis and differentiation of other epidermal appendages are also observed, including the hair follicles. PMID: 17880691
NRG3 was expressed in 42% of breast cancers studied. It was preferentially expressed in breast tumors co-expressing EGFR/HER4. PMID: 17962208
The result of tis study supports that the NRG3 gene is a susceptibility gene for schizophrenia. PMID: 18708184
NRG3 is primarily expressed in the central nervous system and is one of three paralogs of NRG1, a gene strongly implicated in SZ. PMID: 19118813
Observational study and genome-wide association study of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 18846501

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.