Recombinant Human Potassium Channel Subfamily K Member 2 (KCNK2) Protein (His/Tag-Free)

Name: Recombinant Human Potassium Channel Subfamily K Member 2 (KCNK2) Protein (His/Tag-Free)
Brand: Beta LifeScience
SKU: BLC-08197P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Human Potassium Channel Subfamily K Member 2 (KCNK2) Protein (His/Tag-Free) is produced by our Yeast expression system. This is a protein fragment.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	O95069
Target Symbol	KCNK2
Synonyms	hTREK 1c; hTREK 1e; K2p2.1; K2P2.1 potassium channel; KCNK 2; Kcnk2; KCNK2_HUMAN; MGC126742; MGC126744; Outward rectifying potassium channel protein TREK 1; Outward rectifying potassium channel protein TREK-1; Outward rectifying potassium channel protein TREK1; Potassium channel subfamily K member 2; Potassium inwardly rectifying channel subfamily K member 2; Tandem pore domain potassium channel TREK 1; Tandem pore domain potassium channel TREK1; TPKC1; TREK 1; TREK 1 K(+) channel subunit; TREK; TREK-1 K(+) channel subunit; TREK1; TWIK related potassium channel 1; Two pore domain potassium channel TREK 1 ; Two pore domain potassium channel TREK-1; Two pore domain potassium channel TREK1; Two pore potassium channel 1; Two pore potassium channel TPKC1
Species	Homo sapiens (Human)
Expression System	Yeast
Tag	N-His/Tag-Free
Protein Length	Partial
Mol. Weight	17.7kDa
Research Area	Neuroscience
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Ion channel that contributes to passive transmembrane potassium transport. Reversibly converts between a voltage-insensitive potassium leak channel and a voltage-dependent outward rectifying potassium channel in a phosphorylation-dependent manner. In astrocytes, forms mostly heterodimeric potassium channels with KCNK1, with only a minor proportion of functional channels containing homodimeric KCNK2. In astrocytes, the heterodimer formed by KCNK1 and KCNK2 is required for rapid glutamate release in response to activation of G-protein coupled receptors, such as F2R and CNR1.; Does not display channel activity but reduces the channel activity of isoform 1 and isoform 2 and reduces cell surface expression of isoform 2.
Subcellular Location	[Isoform 1]: Cell membrane; Multi-pass membrane protein.; [Isoform 2]: Cell membrane; Multi-pass membrane protein.; [Isoform 4]: Endoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families	Two pore domain potassium channel (TC 1.A.1.8) family
Database References	HGNC: 6277 OMIM: 603219 KEGG: hsa:3776 STRING: 9606.ENSP00000394033 UniGene: Hs.497745
Tissue Specificity	Isoform 4 is detected in kidney, adrenal gland and brain where it is preferentially expressed in the amygdala but not found in thalamus, hypothalamus, hippocampus or substantia nigra.

Gene Functions References

data reveal a druggable K2P site that stabilizes the C-type gate 'leak mode' and provide direct evidence for K2P selectivity filter gating PMID: 28693035
decreased TREK-1 expression in the aganglionic and ganglionic bowel observed in Hirschsprung's disease may alter intestinal epithelial barrier function leading to the development of enterocolitis PMID: 27384506
Atrial TREK-1 expression was reduced in atrial fibrillation patients with concomitant severe heart failure PMID: 28005193
The authors identified a heterozygous point mutation in the selectivity filter of the stretch-activated K2P potassium channel TREK-1 (KCNK2 or K2P2.1). This mutation introduces abnormal sodium permeability and stretch-activation hypersensitivity to TREK-1. PMID: 28242754
downregulated in overactive detrusor PMID: 28539337
The M2-glycine hinge controls the macroscopic currents of TREK1 channels. PMID: 28676394
TREK-1 overexpression suppresses CHO cell proliferation by inhibiting the activity of PKA and p38/MAPK signaling pathways and subsequently inducing G1 phase cell arrest. PMID: 27397543
Trek1 expression facilitates the restoration of intestinal epithelial barrier functions in an allergic environment. PMID: 25683610
presence of TREK-1 variants correlated to reduced TREK-1 activity, suggesting a pathological role for TREK-1 variants in preterm labor PMID: 26400398
Data suggest that potassium channel protein TREK-1 (TREK-1) might be a biomarker in castration resistance free survival (CRFS) judgment of prostate cancer (PCa), as well as a potential therapeutic target. PMID: 25962960
During conductance simulation experiments, both TASK-3 and TREK-1 channels were able to repolarise the membrane once AP threshold was reached PMID: 25482670
How ion channels sense mechanical force: insights from mechanosensitive K2P channels TRAAK, TREK1, and TREK2. PMID: 26332952
We conclude that Trek1 is critical to maintain the nasal epithelial barrier function. PMID: 25778785
Nasal epithelia express Trek1 that can be suppressed by allergic response. PMID: 25529528
Modulation of K2P 2.1 and K2P 10.1 K(+) channel sensitivity to carvedilol by alternative mRNA translation initiation PMID: 25168769
Response of the human detrusor to stretch is regulated by TREK-1 PMID: 24801307
PLD2, but not PLD1, directly binds to the C terminus of TREK1 and TREK2. PMID: 25197053
results suggest that the TREK-1e splice variant may interfere with the vesicular traffic of full-length TREK-1 channels from the ER to the plasma membrane. PMID: 24196565
TREK-1 deficient alveolar epithelial cells have less F-actin and are more deformable making them more resistant to stretch-induced injury. PMID: 24586773
Activation of K(2)P channel-TREK1 mediates the neuroprotection induced by sevoflurane preconditioning. PMID: 24154701
High TREK-1 expression is associated with epithelial ovarian cancer. PMID: 23479219
A number of mutations that affect TREK1 channel gating occlude the action of fenamates but only in the longer form of TREK1. PMID: 24509840
In human embryonic kidney (HEK-293) cells stably expressing TREK-1, outward currents at 80 mV increased from 91.0 +/- 23.8 to 247.5 +/- 73.3 pA/pF. PMID: 23804201
study analyzed the role of TWIK-related potassium channel-1 (TREK1)in endothelial cells and the blood-brain barrier(BBB); blocking TREK1 increased leukocyte transmigration; TREK1 activation had the opposite effect PMID: 23933981
TASK and TREK-1 are involved in regulation of cell proliferation and in control of resting membrane potentials in endometrial epithelial cells. PMID: 23305490
we report the expression of Trek-1 in human alveolar epithelial cells and propose that Trek-1 deficiency may alter both IL-6 translation and transcription in AECs without affecting Ca(2+) signaling PMID: 23275623
each of four TREK-1 splice variants interacts with full-length wild-type TREK-1 and in vivo, such interactions may contribute to a preterm labor phenotype. PMID: 22811574
TREK-1 is expressed by both nucleus pulposus and annulus fibrosus cells of the human intervertebral disc. PMID: 22563662
Cochlin interacts with TREK-1 and annexin A2. PMID: 21886777
the important role of selectivity filter gating in the regulation of TREK-1 by the extracellular K(+) and proton. PMID: 21965685
These results demonstrate that the primary activation mechanisms in TREK-1 reside close to, or within the selectivity filter and do not involve gating at the cytoplasmic bundle crossing. PMID: 21822218
The data of this study suggested that TREK-1 is associated with NSC proliferation and probably is a modulator of the effect that fluoxetine attenuates the inhibitory neurogenesis induced by glucocorticoid hormones. PMID: 21069514
inhibition of TREK1 current by fluoxetine is found to be accompanied by dissociation of the C-terminal domain from the membrane. PMID: 21262820
role for TREK-1 in contributing to uterine quiescence during gestation PMID: 20811500
these data suggest that beta-COP plays a critical role in the forward transport of TREK1 channel to the plasma membrane. PMID: 20362547
Results describe the regulation of neuronal K(2P)2.1 (KCNK2, TREK-1) channel activity by resting membrane potential. PMID: 19837167
These findings indicate that TREK1 genotypes are associated with individual differences in reward-related brain activity. PMID: 19621370
TREK-1 channels may function as sensors that couple the metabolic state of the cell to membrane potential, perhaps through an associated ATP-binding protein PMID: 12368289
During hypoxia, modulation of hTREK1 cannot be accomplished by parameters known to be perturbed in brain ischemia. hTREK1 regulation in brain will be more relevant during alkalosis than during ischemia or acidosis. PMID: 14522822
TREK-1 is inhibited by fluoxetine and norfluoxetine. PMID: 15685212
hypoxic inhibition: (a) requires the C-terminal domain of the channel; (b) does not involve redox modulation of the C-terminal domain cysteine residues C365 and C399; and (c) is critically dependent on the glutamate residue at position 306 PMID: 15883010
receptor- and kinase-induced inhibition of TREK-1 background potassium channels is mediated by sequential phosphorylation PMID: 16006563
human osteoblasts functionally express TREK-1 and that these channels contribute, at least in part, to the resting membrane potential of human osteoblast cells. We hypothesise a possible role for TREK-1 in mechanotransduction, leading to bone remodelling. PMID: 16250016
VOCCs and TREK channels have been implicated in mechanotransduction signaling pathways in numerous connective tissue cell types. PMID: 17035301
TREK1, the most thoroughly studied K(2P) channel, has a key role in the cellular mechanisms of neuroprotection, anaesthesia, pain and depression--{REVIEW} PMID: 17375039
These findings indicate that genetic variation in KCNK2 may identify individuals at risk for treatment resistance. More broadly, they indicate the utility of animal models in identifying genes for pharmacogenetic studies of antidepressant response. PMID: 18288090
voltage-dependent C-type gating acceleration by protons represents a novel mechanism for K2P2.1 outward rectification. PMID: 18474599
(Review) KCNK2, the gene encoding K2P2.1, can generate either full-length or K2P2.1D1-56 via alternative translation initiation, a mechanism which increases protein diversity by giving rise to two or more proteins from a single mRNA strand. PMID: 18579071
Both TASK-3 and TREK-1 are functionally operational in the adrenocortical H295R cell line, modulate membrane potential and aldosterone secretion. PMID: 18854423
Results highlight the important role of K(2P)2.1 channels as receptors for mediators known to cause nociception. PMID: 19130888

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