Recombinant Human PKC eta Protein

Name: Recombinant Human PKC eta Protein
Brand: Beta LifeScience
SKU: BL-0950SG
Availability: InStock

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Product Overview

Tag	GST
Host Species	Human
Accession	NM_006255
Synonym	PKCL; PKC-L; PRKCL; MGC5363; MGC26269; nPKC-eta, PRKCH
Background	PKCη is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases that can phosphorylate a wide variety of protein targets known to be involved in diverse cellular signaling pathways. PKCη is predominantly expressed in squamous cell epithelia and induces terminal differentiation of keratinocytes. PKCη that is endogenously expressed or overexpressed is found to associate with the cyclin E/cdk2/p21 complex in keratinocytes of mice and humans (1).
Description	Recombinant full-length human PKCeta was produced by baculovirus in Sf9 insect cells using a N-terminual GST tag. This protein is purified with our unique purification methods.
Source	Sf9 insect cells
AA Sequence	Full Length
Molecular Weight	~103 kDa
Purity	For specific purity information on a given lot, see related COA.
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Active
Formulation	Recombinant protein is supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol.
Stability	The recombinant protein is stable for up to 12 months at -70°C
Usage	For Research Use Only
Storage	Recombinant Human PKC eta Protein should be stored should be stored at < -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in the regulation of cell differentiation in keratinocytes and pre-B cell receptor, mediates regulation of epithelial tight junction integrity and foam cell formation, and is required for glioblastoma proliferation and apoptosis prevention in MCF-7 cells. In keratinocytes, binds and activates the tyrosine kinase FYN, which in turn blocks epidermal growth factor receptor (EGFR) signaling and leads to keratinocyte growth arrest and differentiation. Associates with the cyclin CCNE1-CDK2-CDKN1B complex and inhibits CDK2 kinase activity, leading to RB1 dephosphorylation and thereby G1 arrest in keratinocytes. In association with RALA activates actin depolymerization, which is necessary for keratinocyte differentiation. In the pre-B cell receptor signaling, functions downstream of BLNK by up-regulating IRF4, which in turn activates L chain gene rearrangement. Regulates epithelial tight junctions (TJs) by phosphorylating occludin (OCLN) on threonine residues, which is necessary for the assembly and maintenance of TJs. In association with PLD2 and via TLR4 signaling, is involved in lipopolysaccharide (LPS)-induced RGS2 down-regulation and foam cell formation. Upon PMA stimulation, mediates glioblastoma cell proliferation by activating the mTOR pathway, the PI3K/AKT pathway and the ERK1-dependent phosphorylation of ELK1. Involved in the protection of glioblastoma cells from irradiation-induced apoptosis by preventing caspase-9 activation. In camptothecin-treated MCF-7 cells, regulates NF-kappa-B upstream signaling by activating IKBKB, and confers protection against DNA damage-induced apoptosis. Promotes oncogenic functions of ATF2 in the nucleus while blocking its apoptotic function at mitochondria. Phosphorylates ATF2 which promotes its nuclear retention and transcriptional activity and negatively regulates its mitochondrial localization.
Subcellular Location	Cytoplasm.
Protein Families	Protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily
Database References	HGNC: 9403 OMIM: 601367 KEGG: hsa:5583 STRING: 9606.ENSP00000329127 UniGene: Hs.333907
Associated Diseases	Ischemic stroke (ISCHSTR)
Tissue Specificity	Most abundant in lung, less in heart and skin.

Gene Functions References

These results suggest that PKCeta utilizes the ERK signaling pathway to protect against ubiquitin-mediated proteasomal degradation of Mcl-1 PMID: 28939105
PRKCH rs2230500 gene polymorphism is significantly related to the efficiency in telmisartan therapy (p = 0.02). The PRKCH rs2230500 may influence the antihypertensive efficacy of telmisartan in Chinese EH patients. PMID: 28640647
PRKCH is highly expressed in hematopoietic stem cell (HSC) and is necessary for optimal HSC function. PMID: 28596089
The association between PRKCH 1425G/A and lacunar infarction was independent of traditional stroke risk factors PMID: 25900926
variant genotypes of PRKCH 1425G/A are an independent prognostic factor for ischemic stroke in the final multivariate Cox regression model PMID: 25370935
Meta-analysis indicated that the 1425G/A SNP in PRKCH may contribute to susceptibility of stroke, especially for ischemic stroke. PMID: 25272991
PKCeta promotes senescence through its ability to upregulate the expression of the cell cycle inhibitors p21Cip1 and p27Kip1 and enhance transcription and secretion of IL-6. PMID: 25412309
The 1425G/A polymorphism in PRKCH is not a significant predictor of stroke recurrence in patients with acute ischemic stroke during a 2-year follow-up period. PMID: 24534126
PKC-eta associates with NHE3 and gamma tubulin to promote the cell polarity during migration. PMID: 24788043
These results suggest that siRNA-mediated silencing of PKCeta can be a potent tool to complement existing chemotherapy regimens for treating EBV(+) B lymphoma. PMID: 24784886
the PKC family genes may play a role in the pathogenesis of MS relapse through modulating the association between 25(OH)D and relapse. PMID: 23868949
study demonstrates that type V collagen induces the down-regulation of protein kinase C eta PMID: 22213077
these results suggest that the regulation of PKCeta is unique and PKCepsilon is required for the PKC activator-induced upregulation of PKCeta. PMID: 22892130
The single nucleotide polymorphism (rs2230500) in PRKCH decreases the risk of carotid intima-media thickness. PMID: 22808203
There was loss of the negative correlation in the expression levels of CD3eta and FcepsilonRIgamma genes in CLL patients. PMID: 22664044
Protein kinase Ceta 1425G/A variant was not a risk locus for deep intracerebral hemorrhage phenotype. PMID: 22999931
It was shown that the PKCeta isoform is a negative regulator of the AKT signaling pathway. The IGF-I induced phosphorylation on Ser473 of AKT was inhibited by the PKCeta-induced expression in MCF-7 breast adenocarcinoma cancer cells. PMID: 22305966
A remarkable association of minor alleles (1425G/A and _15) in the PRKCH gene with an elevated risk of coronary artery disease and increased levels of LDL-C in a Chinese population. PMID: 21625852
The present meta-analyses suggested that 1425G/A SNP in PRKCH was associated with ischemic stroke, particularly lacunar infarction, in Chinese and Japanese populations. PMID: 22044875
In the present study, a significant association between the PRKCH 1425G/A polymorphism and SSNHL risk was observed through a nested case-control analysis within the population-based study. PMID: 21756056
study suggests that PKCeta is involved in NF-kappaB signaling leading to drug resistance. PMID: 21820409
Catalytic activity of protein kinase C eta (PKCeta) is not necessary for enlarged and flattened morphology of differentiated human keratinocytes, although it is important for gene expression of the marker proteins. PMID: 21346190
the AA genotype of PRKCH, relative to the G/G genotype, may be a higher risk genotype for severe gastric atrophy PMID: 20602195
Reduced expression of PKCeta may represent a molecular lesion in the development of more aggressive disease of human hepatocellular carcinoma. PMID: 20001341
that PKC eta negatively regulates UV-induced apoptosis through its localization, resistance to cleavage, and the p38 MAPK pathway PMID: 12646210
Minute virus of mice infection causes accumulation of endogenous PKCeta in the nuclear periphery and regulaties this NS1 regulating kinase, thus underlining the tight interconnection between PKC-mediated signaling and the parvoviral life cycle PMID: 12829844
The major result is a clear correlation of PKC eta expression with tumor progression in renal cell carcinoma. PMID: 14666709
There was expression of protein kinase C eta in abnormal muscle fibers. Protein kinase C isoforms may play a role in the pathogenesis of myofibrillar myopathy. PMID: 15159477
PKC-eta targets the Akt and mTOR signaling pathways PMID: 15489897
These studies demonstrate translocation of PKCeta to the nuclear envelope, and suggest that the spatial regulation of PKCeta could be important for its cellular functions including effects on cell cycle control and involvement in tumor promotion. PMID: 16242915
PKC-eta-mediated glioblastoma proliferation involves MEK/mitogen-activated protein (MAP) kinase phosphorylation, activation of ERK and subsequently of Elk-1. PMID: 17146445
Our results provide evidence of the involvement of PRKCH as a susceptibility gene for rheumatoid arthritis in the Japanese population PMID: 17195206
Fission of transport carriers at the trans-Golgi network is dependent on specifically PLCbeta3, which is necessary to activate PKCeta and PKD in that Golgi compartment, via diacylglycerol production. PMID: 17492941
PKCeta functions as an anti-apoptotic protein in Hodgkin's lymphoma-derived cell lines PMID: 17786031
PRKCH mRNA was expressed at lower level in rheumatoid arthritis unrelated patients than in healthy controls, and is not a rheumatoid arthritis major susceptibility genetic factor in the French Caucasian population. PMID: 17957454
PRKCH gene polymorphisms have a role in subcortical silent brain infarction PMID: 18164711
Results establish PKCeta as a unique element in interferon signaling that plays a key and essential role in the generation of the regulatory effects of type I IFNs on normal and leukemic hematopoiesis. PMID: 19211565
nPKCeta positively regulates agonist-induced thromboxane generation with no effects on platelet aggregation. PMID: 19286657
The 1425G/A SNP in PRKCH increases the risk of both ischemic stroke and cerebral hemorrhage in the Chinese population. PMID: 19520989
Data show that PKCeta is an anti-apoptotic protein, acting as a negative regulator of JNK activity. PMID: 19523467
Translational regulation could provide an additional level for controlling the expression of PKC family members. PMID: 19797084
We report that a nonsynonymous SNP in PRKCH increases the risk of cerebral infarction in the general Japanese population. We also found that PKCeta was expressed in vascular endothelial cells and foamy macrophages in human atherosclerotic lesions. PMID: 17206144

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