Recombinant Human PDGF-BB Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0024
Recombinant Human PDGF-BB (2 ug/lane) on SDS-AGE under reducing (R) and non-reducing (NR) conditions. The gel was stained using Coomassie® Blue showing single bands at 13 kDa and 28 kDa, respectively, and purity greater than 96%.
Recombinant Human PDGF-BB (2 ug/lane) on SDS-AGE under reducing (R) and non-reducing (NR) conditions. The gel was stained using Coomassie® Blue showing single bands at 13 kDa and 28 kDa, respectively, and purity greater than 96%.

Recombinant Human PDGF-BB Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0024
/
Size

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Product Overview

Product Name Recombinant Human PDGF-BB Protein
Target Symbol PDGFB
Alternative Names Platelet Derived Growth Factor BB, PDGFBB, Platelet-Derived Growth Factor B, PDGFB, PDGF Subunit B, Epididymis Secretory Sperm Binding Protein, Oncogene SIS, SIS, Proto-Oncogene C-SIS, C-SIS, Becaplermin
Accession Number P01127.1
Expression System E. coli. Disulfide-linked homodimer
Molecular Weight 12.3 kDa (monomer, predicted), 13 kDa and 28 kDa (observed on SDS-PAGE gel stained with Coomassie® Blue under reducing and non-reducing conditions, respectively).
Sequence Ser82-Thr190
Purity >96% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining
Activity Determined by a cell proliferation assay using mouse Balb/c 3T3 cells. The ED50 for this effect is typically 1-3 ng/mL
Formulation Lyophilized from sterile Sodium Acetate and NaCl with Trehalose, without carrier protein.
Endotoxin Level <0.2 EU per 1 µg of the protein as determined by the LAL method
Shipping Shipped at ambient temperature.
Stability & Storage 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.
Reconstitution Reconstitute at 100 ug/mL in sterile 4 mM HCl.
Target Function Platelet-Derived Growth Factor BB (PDGF-BB) is a protein with roles in regulating of many processes including embryonic development, angiogenesis, cell proliferation and differentiation. PDGF-BB acts on mesenchymal cells as a mitogen for connective tissue formation, particularly fibroblasts, vascular smooth muscle cells and glial cells. PDGF-BB is present in the alpha granules of platelets. As such, PDGF-BB promotes angiogenesis, chemotaxis of fibroblasts and collagen synthesis in tissue repair. Additionally, PDGF-BB supports development of kidney glomeruli, the differentiation of neural stem cells and has neuroprotective functions on mature neurons during injury. PDGF-BB primarily binds to the PDGFR beta (CD140B) homodimer, and with lower affinity to the PDGFR alpha (CD140a) homodimer or PDGFR alpha/PDGFR beta heterodimer leading to activation of the MAPK, PI3K, and STAT3 pathways.
Tissue Specificity PDGF-BB expression has been identified in endothelial tissue, smooth muscle tissue, heart, lung, brain, and neurons.
Cellular Localization Secreted protein
Involvement In Disease Dysregulation of PDGF-BB is associated with atherosclerosis, pulmonary hypertension, glomerulonephritis, diabetic retinopathy, keloids, hypertrophic scars, cancer (glioma, sarcoma, and tumor angiogenesis).

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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