Recombinant Human Oxysterols Receptor Lxr-Alpha (NR1H3) Protein (His-SUMO)

Name: Recombinant Human Oxysterols Receptor Lxr-Alpha (NR1H3) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-08876P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Oxysterols Receptor Lxr-Alpha (NR1H3) Protein (His-SUMO) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	Q13133
Target Symbol	NR1H3
Synonyms	Liver X receptor alpha; LXR a; LXRA; NR1H3; NR1H3_HUMAN; Nuclear receptor subfamily 1 group H member 3; Oxysterols receptor LXR alpha; Oxysterols receptor LXR-alpha; RLD 1; RLD1
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	MPHSAGGTAGVGLEAAEPTALLTRAEPPSEPTEIRPQKRKKGPAPKMLGNELCSVCGDKASGFHYNVLSCEGCKGFFRRSVIKGAHYICHSGGHCPMDTYMRRKCQECRLRKCRQAGMREECVLSEEQIRLKKLKRQEEEQAHATSLPPRASSPPQILPQLSPEQLGMIEKLVAAQQQCNRRSFSDRLRVTPWPMAPDPHSREARQQRFAHFTELAIVSVQEIVDFAKQLPGFLQLSREDQIALLKTSAIEVMLLETSRRYNPGSESITFLKDFSYNREDFAKAGLQVEFINPIFEFSRAMNELQLNDAEFALLIAISIFSADRPNVQDQLQVERLQHTYVEALHAYVSIHHPHDRLMFPRMLMKLVSLRTLSSVHSEQVFALRLQDKKLPPLLSEIWDVHE
Expression Range	1-402a
Protein Length	Full Length of Isoform 3
Mol. Weight	61.7kDa
Research Area	Transcription
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity. Interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism. Induces LPCAT3-dependent phospholipid remodeling in endoplasmic reticulum (ER) membranes of hepatocytes, driving SREBF1 processing and lipogenesis. Via LPCAT3, triggers the incorporation of arachidonate into phosphatidylcholines of ER membranes, increasing membrane dynamics and enabling triacylglycerols transfer to nascent very low-density lipoprotein (VLDL) particles. Via LPCAT3 also counteracts lipid-induced ER stress response and inflammation, likely by modulating SRC kinase membrane compartmentalization and limiting the synthesis of lipid inflammatory mediators.
Subcellular Location	Nucleus. Cytoplasm.
Protein Families	Nuclear hormone receptor family, NR1 subfamily
Database References	HGNC: 7966 OMIM: 602423 KEGG: hsa:10062 STRING: 9606.ENSP00000387946 UniGene: PMID: 29367626 LXR-alpha played a central role in down-regulating of ABCA1 and ABCG1 and lipid accumulation induced by homocysteine in the macrophages. PMID: 30393020 LXRalpha interacts with OGT in its N-terminal domain and ligand-binding domain (LBD) in a ligand-independent fashion. PMID: 29577901 Our data suggest that LXR could be used as a biomarker for hepatocellular carcinoma prognosis. PMID: 28508927 PBMCs from healthy persons were predisposed to the MPhi2 differentiation phenotype, which exhibits elevated cholesterol uptake and anti-inflammatory properties. LXRalpha over-expression polarizes macrophages towards the anti-inflammatory MPhi2 phenotype. PMID: 29420090 LXR gene expression was significantly increased in obese children with obstructive sleep apnea-hypopnea syndrome (OSAHS). The severity of OSAHS was positively correlated with COX-2 levels. PMID: 28676625 In conclusion, the present study demonstrated that activation of LXRalpha-ABCA1 axis with a synthetic LXR agonist TO90 exerted a potent protective effect against Abeta induced senescent and inflammatory responses in retinal pigment epithelial cells, suggesting that LXR agonists may be promising therapeutic agents for treating age-related macular degeneration. PMID: 28361293 AMPK activates LXRalpha and ABCA1 expression in human macrophages PMID: 27343431 PPARalpha and LXRalpha may be mediators by which omega3PUFA attenuate bile acid-induced hepatocellular injury PMID: 26756785 Inhibition of Pancreatic Cancer Cell-Induced Paracrine Hedgehog Signaling by Liver X Receptor Agonists and Oxy16, a Naturally Occurring Oxysterol PMID: 27490478 Data identify LXR as an important factor in early-pregnancy lipogenesis that is also necessary to protect against abnormalities in fetoplacental lipid homeostasis. PMID: 28420650 data suggest that ASXL3 is another corepressor of LXRalpha, promoting to the regulation of lipid homeostasis PMID: 25450400 results indicated that LXRalpha plays a specific and important role in activation of TH by regulating D1, and that LXRalpha binds to and regulates the hDIO1 promoter, competing with TRbeta on specific sequences within the promoter. PMID: 28617824 GW3965 significantly increases the expression of liver X nuclear receptor beta (LXRbeta) mRNA, while the liver X nuclear receptor alpha (LXRalpha( mRNA expression did not change a lot, and sensitizes gefitinib by inhibiting NF-kappa B (NF-kappaB) activation. PMID: 28178657 Transactivation assays showed that MCFA activated LXRa, whereas long-chain FA caused no effect. Our results suggest that LXRa functions as a receptor for saturated FA or acyl-CoA of C10 and C12 in length. PMID: 28011707 We demonstrated that LXR stimulation decreases mRNA and protein expression of FLOT2 and DHHC5 in MCF-7 cells. LXR stimulation also reduces Akt phosphorylation and its localization at the plasma membrane PMID: 28739689 The effects of LXR agonist on interleukin-8 (IL-8) secretion and nuclear factor-kappa B (NF-kappaB) activation in human umbilical vein endothelial cells (HUVECs), is reported. PMID: 27489081 Collectively, these findings demonstrate that LXRalpha activation induces 17beta-HSD13 expression in a SREBP-1c-dependent manner. PMID: 28270440 mutant NR1H3 (LXRA) alters gene expression profiles, suggesting a disruption in transcriptional regulation as one of the mechanisms underlying Multiple Sclerosis pathogenesis. PMID: 27253448 Data show that the synthetic liver X receptors (LXRs) agonist T0901317 promoted cytokines IL-1beta, IL-6 and TNFalpha mRNA degradation, destabilized TNFalpha mRNA through its 3'-untranslated region, and increased the expression of tristetraprolin (TTP). PMID: 28119310 RXRalpha negatively regulates the transcription and expression by directly binding to the RARE in the promoter of Cx43 PMID: 26991262 Serum and placental LXR-alpha and endoglin levels were significantly higher in patients with preeclampsia than those in control group (P<0.05, each). PMID: 27736929 this study shows that the anti-inflammatory effect of platycodin D in IL-1beta-stimulated chondrocytes is mediated by activating LXR-a PMID: 27743553 results indicated that down-regulation of LXRalpha was shown to suppress invasion and EMT of gastric cancer cells PMID: 28091828 this study shows that show that H2O2 exerts a dual regulation on mRNA expression of LXRalpha and its target genes PMID: 27351826 combined PPARgamma C1431T, PGC-1alpha G482S, and LXRalpha -115G/A polymorphisms increase the risk of coronary artery disease and predicted the severity of coronary atherosclerosis in Thais PMID: 27016616 Distinct gene regulatory programs define the inhibitory effects of liver X receptors, NR1H2/NR1H3 and PPARG on cancer cell proliferation. PMID: 27401066 these data show that YXS is effective in mitigating MI/R injury by suppressing mitochondrial mediated apoptosis and oxidative stress and by upregulating LXRalpha, thereby providing a rationale for future clinical trials and clinical applications PMID: 26964694 decreased expression of miR-155 in the peripheral blood may be utilized as a potential novel biomarker for non-alcoholic fatty liver disease screening mainly by targeting LXRalpha. PMID: 27832630 The anti-inflammatory effects of platelet-derived microparticles in human plasmacytoid dendritic cells involve liver X receptor activation. PMID: 26635040 these data identify a new mechanism of LXR regulation that involves TIPARP, ADP-ribosylation and MACROD1. PMID: 26814197 Intestinal activation of LXR reduces the production of chylomicrons by a mechanism dependent on the apical localization of SR-B1. PMID: 26602218 Suggest that blocking cholesterol deposition and inhibiting the LXRalpha pathway-induced inflammatory response might be one of the main mechanisms by which anthocyanins exert their protective effects in diabetic nephropathy. PMID: 26379423 LXR-alpha might downregulate S1PR2 expression via miR-130a-3p in quiescent HUVECs. Stimulation of TNF-alpha attenuates the activity of LXR-alpha and results in enhanced S1PR2 expression. PMID: 26669941 Lipoxin A4 increases ABCA1 expression and promotes cholesterol efflux through LXRalpha pathway in THP-1 macrophage-derived foam cells. PMID: 26261553 These results identify LXRalpha as a key cardiac transcriptional regulator that helps orchestrate an adaptive metabolic response to chronic cardiac stress. PMID: 26160456 Studied the role of LXRalpha with Wnt/beta-catenin signaling in adipogenesis of MSCs. PMID: 26595172 In conclusion, our data indicate that HNF-4alpha may have a wider role in cell and plasma cholesterol homeostasis by controlling the expression of LXRalpha in hepatic cells. PMID: 26692490 Propofol up-regulates expression of ABCA1, ABCG1, and SR-B1 through the PPARgamma/LXRalpha pathway in THP-1 macrophage-derived foam cells. PMID: 25600616 The rs12221497 polymorphism in the LXRalpha gene was associated with the susceptibility to stroke in a Han Chinese population. PMID: 25867319 Data show that menin, encoded by the MEN1 gene, inhibits the transcriptional activity of nuclear receptor liver X receptor alpha (LXRalpha). PMID: 25962847 the rsl2221497 polymorphism in the LXRalphagene was associated with the susceptibility to stroke in a Chinese population. PMID: 25729942 treatment with Ang-(1-7) promoted cholesterol efflux in Ang II-treated THP-1 macrophages, partly through inactivation of p38 and JNK signaling and by inducing the expression of PPARg and LXRa. PMID: 25779847 LXRb is the dominant isoform in the rat myocardium and the expression of both LXR isoforms (LXRa and LXRb) did not change after administration of T0901317 PMID: 25659329 The data indicate a direct inhibitory interaction of polyunsaturated fatty acids with LXRalpha, a consequent reduction of SREBP-1 and of its binding to SCD1 promoter. PMID: 25264165 NR1H3 accelerates hepatic differentiation through an HNF4alpha-dependent reciprocal network. PMID: 25073010 This study provides the first evidence to show LXR activation reduces cadmium-induced apoptotic cell death of human renal proximal tubular cells by inhibition of reactive oxygen species production and JNK activation. PMID: 25980575 Activation of LXRs interfered with the release of interleukin-6 from macrophages and, thus, inhibited fibroblast activation and collagen release. PMID: 24618263 ligands selectively regulate placenta gene targets and functional pathways PMID: 25255963 An increase of 55% in LXR-alpha gene expression at RNA level was observed in Atorvastatin + 22-R hydroxycholestrol compared to 24% in Ascorbic acid + 22-ROH cholesterol. PMID: 25283515

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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