Recombinant Human Orexin Receptor Type 2 (HCRTR2) Protein (His/Tag-Free)

Name: Recombinant Human Orexin Receptor Type 2 (HCRTR2) Protein (His/Tag-Free)
Brand: Beta LifeScience
SKU: BLC-08195P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Orexin Receptor Type 2 (HCRTR2) Protein (His/Tag-Free) is produced by our Yeast expression system. This is a protein fragment.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	O43614
Target Symbol	HCRTR2
Synonyms	Hcr tr2; Hcrt receptor 2; Hcrtr2; Hypocretin (orexin) receptor 2; Hypocretin receptor 2; Hypocretin receptor type 2; Orexin 2 receptor; Orexin A/Orexin B receptor; Orexin receptor 2; Orexin receptor type 2; Orexin type 2 receptor; Ox-2-R; Ox2-R; Ox2r; OX2R_HUMAN; Type 2 hypocretin receptor; Type 2 orexin receptor
Species	Homo sapiens (Human)
Expression System	Yeast
Tag	N-His/Tag-Free
Protein Length	Partial
Mol. Weight	10.8kDa
Research Area	Cardiovascular
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Nonselective, high-affinity receptor for both orexin-A and orexin-B neuropeptides. Triggers an increase in cytoplasmic Ca(2+) levels in response to orexin-A binding.
Subcellular Location	Cell membrane; Multi-pass membrane protein.
Protein Families	G-protein coupled receptor 1 family
Database References	HGNC: 4849 OMIM: 602393 KEGG: hsa:3062 STRING: 9606.ENSP00000359899 UniGene: PMID: 29959630 Association between HCRTR2, ADH4,CLOCK gene polymorphisms and cluster headache was not significant in the present study. PMID: 29318394 The present study demonstrated that aa residues in the C-terminus serve an important role in the expression and signal transduction of OX2R. In addition, the aa residues in different locations possess evidentially different roles. PMID: 28487995 This study showed that Lack of Association between Genetic Polymorphism of ox2r gene with Late Onset Depression and Alzheimer's Disease in a Sample of a Brazilian Population PMID: 27335043 G-protein-dependency of OX2 receptor signalling PMID: 27237973 Rapid-onset obesity with hypothalamic dysfunction, hypoventilation, and autonomic dysregulation is highly unlikely to be caused by mutations in the exons of the HCRTR2 gene. PMID: 26555080 The study determined structures of OX1R bound to the OX1R-selective antagonist SB-674042 and the dual antagonist suvorexant at 2.8-A and 2.75-A resolution and explored mechanisms of antagonist the subtype selectivity between OX1R and OX2R. PMID: 26950369 our meta-analysis provides genetic evidence for a role of HCRTR2 in cluster headache PMID: 25398231 Heart failure patients with minor allele for rs7767652, upstream of hypocretin (orexin) receptor-2 (HCRTR2), were less likely to have improved left ventricular function and a lower prevalence of ejection fraction >35% PMID: 26653627 the nonsynonymous rs2653349 single nucleotide polymorphism (SNP) (located on the gene that encodes orexin [hypocretin] receptor 2) was selected as the most notable SNP associated with Fagerstrom Test for Nicotine Dependence. PMID: 26289589 Antibodies from vaccine-associated narcolepsy sera cross-reacted with both influenza nucleoprotein and hypocretin receptor 2. PMID: 26136476 The genotypic and allelic frequencies of the rs2653349 polymorphism were different between Alzheimer's disease patients and controls. The carriage of the G allele was associated with an increased AD risk. PMID: 24969517 using lipid-mediated crystallization and protein engineering with a novel fusion chimaera, the structure of the human OX2R bound to suvorexant at 2.5 A resolution is solved PMID: 25533960 DQ B1 but not HLA-DR typing, TNF-alpha levers, HCRTR1 and HCRTR2 were higher in narcolepsy-cataplexy/schizophrenia patients. PMID: 24268496 Orexinergic innervation and expression of genes encoding OX2R receptor are upregulated in transgenic mice at dusk. PMID: 24599460 Expression of the OX2R protein was detected in normal endometrial epithelia, whereas it was frequently lacking in endometrial endometrioid carcinoma. PMID: 23482607 Data demonstrated that hOX2R expression is regulated by a complex involving a proximal PKA/PKC-regulated promoter and a distal promoter regulating tissue-specific expression of alternative transcripts which in turn regulate receptor levels. PMID: 20156195 The data suggest that the HCRTR2 gene or a linked locus significantly modulates the risk for cluster headache. PMID: 15477554 All adrenal cortex adenomas expressed OX1-R and OX2-R mRNAs, and real-time PCR showed that the expression of both receptors was up-regulated in adenomas PMID: 15797953 Our results do not support a role of the HCRTR2 G1246A polymorphism in drug responses in CH. PMID: 17376114 polymorphism of the HCRTR2 gene may modulate the genetic risk for cluster headache PMID: 17563843 the HCRTR2 gene is not a genetic risk factor in migraine. PMID: 17645762 This study does not support a major contribution of the HCRTR2 G1246A polymorphism to the pathogenesis of migraine in contrast to its effects in cluster headache. PMID: 17883525 Three new polymorphisms of the HCRTR2 gene are significantly associated with cluster headache. PMID: 18399985 This study describes the intracellular signalling pathways involved in OX2R-mediated ERK(1/2) and p38 MAPK activation. PMID: 18599270 Biochemical and behavioural characterization of EMPA, a novel high-affinity, selective antagonist for the OX(2) receptor. PMID: 19751316

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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Recombinant Human Orexin Receptor Type 2 (HCRTR2) Protein (His/Tag-Free)

Recombinant Human Orexin Receptor Type 2 (HCRTR2) Protein (His/Tag-Free)

Product Overview

Target Details

FAQs