Name: Recombinant Human OPG Protein (His Tag)
Brand: Beta LifeScience
SKU: BL-0300NP
Availability: InStock

Catalog No.: BL-0300NP

Product Overview

Tag	His
Host Species	Human
Accession	O00300
Synonym	OPG Tumor Necrosis Factor Receptor Superfamily Member 11B; Osteoclastogenesis Inhibitory Factor; Osteoprotegerin; TNFRSF11B; OCIF; OPG
Background	TNFRSF11B is a secreted protein, containing 2 death domains and 4 TNFR-Cys repeats. TNFRSF11B is a decoy receptor for the receptor activator of nuclear factor kappa B ligand (RANKL). By binding RANKL, TNFRSF11B inhibits nuclear kappa B (NF-κB) which is a central and rapid acting transcription factor for immune-related genes, and a key regulator of inflammation, innate immunity, and cell survival and differentiation. TNFRSF11B levels are influenced by voltage-dependent calcium channelsCav1.2. TNFRSF11B can reduce the production of osteoclasts by inhibiting the differentiation of osteoclast precursors (osteoclasts are related to monocytes/macrophages and are derived from granulocyte/macrophage-forming colony units (CFU-GM)) into osteoclasts and also regulates the resorption of osteoclasts in vitroand in vivo. TNFRSF11B binding to RANKL on osteoblast/stromal cells, blocks the RANKL-RANK ligand interaction between osteoblast/stromal cells and osteoclast precursors. This has the effect of inhibiting the differentiation of the osteoclast precursor into a mature osteoclast.
Description	Recombinant Human Osteoprotegerin is expressed by our Mammalian expression system and the target gene encoding Glu22-Leu401 is expressed with a His tag at the C-terminus.
Source	Human Cells
AA Sequence	Glu22-Leu401
Purity	>95% as determined by reducing SDS-PAGE.
Endotoxin	<0.1 ng/µg (1 EU/µg) as determined by LAL test.
Bioactivity	Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells. treated with TRAILThe ED50 for this effect is less than 25 ng/ml.
Formulation	Recombinant Human OPG Protein was lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Reconstitution	Dissolve the lyophilized protein in distilled water.
Usage	For Research Use Only
Storage	Human OPG protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.