Recombinant Human Nuclear Receptor Corepressor 2 (NCOR2) Protein (His)

Name: Recombinant Human Nuclear Receptor Corepressor 2 (NCOR2) Protein (His)
Brand: Beta LifeScience
SKU: BLC-07412P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Nuclear Receptor Corepressor 2 (NCOR2) Protein (His) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	Q9Y618
Target Symbol	NCOR2
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-10His
Target Protein Sequence	MSGSTQPVAQTWRATEPRYPPHSLSYPVQIARTHTDVGLLEYQHHSRDYASHLSPGSIIQPQRRRPSLLSEFQPGNERSQELHLRPESHSYLPELGKSEMEFIESKRPRLELLPDPLLRPSPLLATGQPAGSEDLTKDRSLTGKLEPVSPPSPPHTDPELELVPPRLSKEELIQNMDRVDREITMVEQQISKLKKKQQQLEEEAAKPPEPEKPVSPPPIESKHRSLVQIIYDENRKKAEAAHRILEGLGPQVELPLYNQPSDTRQYHENIKINQAMRKKLILYFKRRNHARKQWEQKFCQRYDQLMEAWEKK
Expression Range	1-312aa
Protein Length	Partial
Mol. Weight	42.3 kDa
Research Area	Cancer
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Transcriptional corepressor. Mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription. Isoform 1 and isoform 4 have different affinities for different nuclear receptors. Involved in the regulation BCL6-dependent of the germinal center (GC) reactions, mainly through the control of the GC B-cells proliferation and survival. Recruited by ZBTB7A to the androgen response elements/ARE on target genes, negatively regulates androgen receptor signaling and androgen-induced cell proliferation.
Subcellular Location	Nucleus.
Protein Families	N-CoR nuclear receptor corepressors family
Database References	HGNC: 7673 OMIM: 600848 KEGG: hsa:9612 STRING: 9606.ENSP00000384018 UniGene: PMID: 30157580 The pregnane X receptor (PXR) and the nuclear receptor corepressor 2 (NCoR2) modulate cell growth in head and neck squamous cell carcinoma PMID: 29470550 Cellular Differentiation of Human Monocytes Is Regulated by Time-Dependent Interleukin-4 Signaling and the Transcriptional Regulator NCOR2. PMID: 29262348 this study identifies NCOR2 as a new gene for FVC, indicating the importance of further research into the role of vitamin A intake/supplementation and its interactions with related genes in the regulation of FVC. PMID: 26836265 Molecular basis for the specific interactions between HDAC4 and the SMRT corepressor. PMID: 26161557 SNPs in Notch pathway genes may be predictors of cutaneous melanoma disease-specific survival. PMID: 25953768 Significant methylation changes in the SLC23A2 and NCOR2 regulatory regions. PMID: 25821969 A repeated peptide motif present in both SMRT and NCoR is sufficient to mediate specific interaction, with micromolar affinity, with all the Class IIa Histone Deacetylases (HDACs 4, 5, 7, and 9). PMID: 26055705 SMRT enhances cell growth of estrogen receptor-alpha-positive breast cancer cells by promotion of cell cycle progression and inhibition of apoptosis. PMID: 24971610 Phosphorylation of the CK2 site on SMRT significantly increased affinity for SHARP. PMID: 24268649 SMRT is responsible for basal repression of Wip1,a phosphatase that de-phosphorylates and inactivates Chk2, thus affecting a feedback loop responsible for licensing the correct timing of Chk2 activation and the proper execution of the DNA repair process. PMID: 23690919 SMRT may be recruited in the SXR-cofactor complex even in the presence of ligand; SMRTmay be involved not only in SXR-mediated suppression without ligand, but also in ligand-activated transcription to suppress the overactivation of transcription PMID: 23562850 There was no association between SMRT expression and overall survival for patients, regardless of whether they received tamoxifen PMID: 23015261 expression of the transcriptional corepressor complex subunits GPS2 and SMRT was significantly reduced in obese adipose tissue, inversely correlated to inflammatory status PMID: 23221346 Both MEK1 and SMRT bind to the c-Fos promoter and regulate its transcription; SMRT knockdown results in early-phase stimulation followed by a late-phase inhibition of T cell activation. PMID: 23225884 Thyroid hormone receptors induce TRAIL expression, and TRAIL thus synthesized acts in concert with simultaneously synthesized Bcl-xL to promote metastasis PMID: 22576662 Our findings highlight a novel splice variant of NCOR2 as a candidate biomarker in breast cancer that not only predicts tamoxifen response but may be targeted to overcome tamoxifen resistance PMID: 23117886 SMRT and GR act in a consistent manner with steroid hormones PMID: 23055525 altered recruitment and loss of corepressors SMRT/NCoR may provide a mechanism that changes the response of AR function to ligands and contributes to the progression of the advanced stages of human prostate cancer. PMID: 22695118 miR-16 targets SMRT and modulates NF-kappaB-regulated transactivation of the IL-8 gene. PMID: 22292036 An equilibrium dissociation constant obtained for SMRT in the presence and absence of rifampicin indicates that the ligand does not enhance the affinity of the pregnane X receptor or its corepressor. PMID: 22185585 Data report that SMRT interacts with itself to form a protein dimer, and that Erk2, a mitogen-activated protein (MAP) kinase, disrupts this SMRT self-dimerization in vitro and in vivo. PMID: 20965228 The crystal structure of the tetrameric oligomerization domain of TBL1, which interacts with both SMRT and GPS2, and the NMR structure of the interface complex between GPS2 and SMRT. PMID: 21240272 This study independently verifies the influence of NCOR2 and IDH1 on HIV transmission, and its findings suggest that variation in these genes affects susceptibility to HIV infection in exposed individuals. PMID: 21083371 Aberrant expression and modification of SMRT might be involved in the pathogenesis of tumoral cortisol resistance. PMID: 20555024 Tamoxifen treatment of breast cancer cells reduced the expression of ER-alpha and increased the expression of SMRT. PMID: 18546531 SMRT decreased with oestradiol treatment in human skeletal muscle cells PMID: 19432593 Sstable binding of the Stat5-RARalpha fusion protein to corepressor SMRT is accompanied by an impaired response to differentiation signals in hematopoietic cells PMID: 11929749 Interactions that determine the assembly of a retinoid X receptor/corepressor complex PMID: 11972046 The silencing mediator of retinoic acid and thyroid hormone receptors can interact with the aryl hydrocarbon (Ah) receptor but fails to repress Ah receptor-dependent gene expression. PMID: 12139968 SMRT has a role as a coactivator for thyroid hormone receptor T3Ralpha from a negative hormone response element PMID: 12388540 a significant role of SMRT in modulating androgen receptor transcriptional activity PMID: 12441355 Differing transcriptional properties appear to reflect the differing abilities of the three RAR isotypes to interact with the SMRT corepressor protein. PMID: 12554770 SMRT and DAX-1 repress agonist-dependent activity of both androgen and progesterone receptors PMID: 12771131 SANT motif interprets the histone code and promotes histone deacetylation PMID: 12840002 Data describe a 17 residue fragment from SMRT that binds to the BCL6 BTB domain, and report the crystal structure of the complex to 2.2 angstroms. PMID: 14690607 Elevated SMRT levels are common in prostate cancer cells, resulting in suppression of target genes associated with antiproliferative action. PMID: 15300237 SMRTbeta expression may influence the binding and transcriptional capacities of nuclear receptors in tumor cells (SMRTbeta) PMID: 15319284 differential mRNA splicing of SMRT serves to customize corepressor function in different cells, allowing the transcriptional properties of nuclear receptors to be adapted to different contexts PMID: 15632172 No significant allelic/genotypic association between any of the five mutations in SMRT/N-CoR2 and bipolar phenotype and the CAG repeat did not demonstrate allelic instability. PMID: 15635693 the subnuclear positioning of SMRT is influenced by the ligand-bound ERalpha, and this activity is dependent on the ratio of the co-expressed ERalpha and SMRT PMID: 15713534 TRAC-1 (T cell RING protein identified in activation screen)is the first E3 ubiquitin ligase that serves a positive regulatory role in T cell activation. PMID: 15843525 The aryl hydrocarbon receptor activates the retinoic acid receptoralpha through SMRT antagonism PMID: 16480812 expression of the silencing mediator of retinoid and thyroid receptor (SMRT) & histone deacetylase4 (HDAC4) enhances formation of Bach2 foci in nuclear matrix. SMRT mediates HDAC4 binding to Bach2, & HDAC4 facilitates retention of Bach2 in the foci. PMID: 17383980 NCOR2/SMRT is associated with poor patient outcome in breast cancer PMID: 17902051 These results would shed new insights for the molecular mechanisms of PML-RARalpha-associated leukemogenesis. PMID: 17991421 CD40 signaling rapidly disrupts the ability of BCL6 to recruit the SMRT corepressor complex by excluding it from the nucleus, leading to histone acetylation, RNA polymerase II processivity, and activation of BCL6 target genes PMID: 18487509 SMRT protein stability is regulated by Pin1 and Cdk2. PMID: 18838553 Activated MEK signaling cascade inhibits functional recruitment of corepressor SMRT to cyproterone acetate-bound AR in prostate cancer cells. PMID: 19223455 SMRT function restoration induces JAG2 down-regulation as well as multipe myeloma apoptosis. PMID: 19417136

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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