Recombinant Human Neuronal Migration Protein Doublecortin (DCX) Protein (His-SUMO)

Name: Recombinant Human Neuronal Migration Protein Doublecortin (DCX) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-09498P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Neuronal Migration Protein Doublecortin (DCX) Protein (His-SUMO) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	O43602
Target Symbol	DCX
Synonyms	DBCN; Dbct; DC; DCX; DCX_HUMAN; Doublecortex; Doublin; FLJ51296; Lis X; Lis-X; Lissencephalin X; Lissencephalin-X; Lissencephaly X linked; Lissencephaly X linked doublecortin; LISX; Neuronal migration protein doublecortin; OTTHUMP00000023859; OTTHUMP00000023860; OTTHUMP00000216315; OTTHUMP00000216316; SCLH; XLIS
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	MKTLPLHSHCTEMQRLLPKLEMLTLGSSFCSLQGEFCQAMDSFTTVSHVGMCEETDASFNVFSPKFQFDRSHCQSLRFHQNMELDFGHFDERDKTSRNMRGSRMNGLPSPTHSAHCSFYRTRTLQALSNEKKAKKVRFYRNGDRYFKGIVYAVSSDRFRSFDALLADLTRSLSDNINLPQGVRYIYTIDGSRKIGSMDELEEGESYVCSSDNFFKKVEYTKNVNPNWSVNVKTSANMKAPQSLASSNSAQARENKDFVRPKLVTIIRSGVKPRKAVRVLLNKKTAHSFEQVLTDITEAIKLETGVVKKLYTLDGKQVTCLHDFFGDDDVFIACGPEKFRYAQDDFSLDENECRVMKGNPSATAGPKASPTPQKTSAKSPGPMRRSKSPADSANGTSSSQLSTPKSKQSPISTPTSPGSLRKHKDLYLPLSLDDSDSLGDSM
Expression Range	1-441aa
Protein Length	Full Length
Mol. Weight	65.3kDa
Research Area	Neuroscience
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration.
Subcellular Location	Cytoplasm. Cell projection, neuron projection.
Database References	HGNC: 2714 OMIM: 300067 KEGG: hsa:1641 STRING: 9606.ENSP00000337697 UniGene: PMID: 28766905 specifically recognizes compacted GDP-like microtubule lattice PMID: 27238282 this suggests that the microtubule-interacting doublecortin domain observed in cryo-electron micrographs is the C-terminal domain rather than the N-terminal one. PMID: 27226599 From this family, we conclude that a DCX mutation causes a pleiotropic phenotype in the female even if X chromosome inactivation pattern is not skewed, and the novel missense mutation in DCX produced relatively mild dysfunction of the doublecortin protein. PMID: 25868952 We identified a novel DCX mutation c.785A > G, p.Asp262Gly in a family with X-linked lissencephaly and subcortical band heterotopia PMID: 27292316 In high-risk metastatic Neuroblastoma, TH and DCX mRNA quantification could be used for the assessment of response to treatment and for early detection of progressive disease or relapses. PMID: 26498952 Results point to a critical role of doublecortin in the formation of the neuromuscular junctions. PMID: 25817838 It was conclude that microtubule ends have two distinct features that proteins can recognize independently, namely a structural feature related to curvature and nucleotide state. PMID: 25283777 DCX-positive cells occur in a wide range of hypothalamic nuclei in humans, mice and sheep. PMID: 24288185 human DCX protein was expressed in human adipose stem cells, collagen II was decreased while aggrecan, matrilin 2, and GDF5 were increased during the 14-day pellet culture. PMID: 24758934 We propose that DCDC2 is a tumor suppressor gene of HCC. PMID: 24034596 in utero doublecortin knockdown, but not knockout, shows a neocortical neuronal migration phenotype. PMID: 24945770 Immunoblots revealed that depressed subjects displayed increased expression of doublecortin. PMID: 23260340 coexpression of DCX and SPARC collaboratively diminished radioresistance of glioma cells. PMID: 23846421 DCX is dispensable for the development of new neurons in adult mice PMID: 23667508 one deleterious mutation in the DCX gene was identified in a 5-year-old girl with lissencephaly spectrum PMID: 23583063 This finding points to the possible implication of mosaic deletions in the DCX gene in unexplained forms of subcortical band heterotopia (SBH) and may allow for detection of SBH prenatally. PMID: 22833188 DCX binds to polymerization intermediates at growing microtubule ends, in support of a mechanism for stabilizing 13-protofilament microtubules. PMID: 22727374 Data report a negative correlation between DCX mRNA expression and white matter neuron density in schizophrenia, in contrast to a positive correlation in human development where DCX mRNA and white matter neuron density are higher earlier in life. PMID: 21966452 Up-regulation of doublecortin is associated with brain damage in children with acute lymphoblastic leukemia PMID: 21846577 DCX synthesis induces apoptosis in brain tumor stem cells (BTSC) through a novel JNK1/neurabin II/DCX/PP1/caspase-3 pathway. PMID: 21477071 study describes male siblings with Lennox-Gastaut syndrome and pachygyria with a novel missense mutation in the DCX gene PMID: 20726879 The DCX binding on the microtubules surface indirectly stabilizes conserved tubulin-tubulin lateral contacts in the microtubules lumen, operating independently of the nucleotide bound to tubulin. PMID: 20974813 variants in doublecortin- and calmodulin kinase like 1, a gene up-regulated by BDNF, have roles in memory and general cognitive abilities PMID: 19844571 Evidence for a role for miR-128 in neuroblastoma progression and aggressiveness through reelin and DCX expression is reported. PMID: 19713529 During development of the cerebral cortex DCX is expressed in both pyramidal and non-pyramidal migrating cells, as well as in Cajal-Retzius cells. PMID: 12427674 Missense DCX mutations may manifest as non-syndromic mental retardation with cryptogenic epilepsy in female subjects and subcortical band heterotopia (SBH) in boys. PMID: 12838518 Different clinical and morphological phenotypes in monozygotic twins with identical DCX mutation. PMID: 14999500 both mutant and wild type DCX are able to bind and bundle microtubules; however, mutants possess a decreased ability to perturb the mitotic machinery, to cause abnormal spindle orientation, and to impair mitotic progression PMID: 15045646 DCX maps at Xq22.3 and is caused by a homozygous mutation. It acts during corticogenesis on radial migratory pathways. PMID: 15057976 We analysed the human DCX regulatory sequence and confined it to a 3.5-kb fragment upstream of the ATG start codon. This fragment is sufficient and specific to drive expression of reporter genes in embryonic and adult neuronal precursors PMID: 15663475 doublecortin (DCX) gene as a new molecular marker of neuroblastoma cells PMID: 15714065 Collectively, the immunohistochemistry, Western blots and Northern blots conclusively demonstrate expression of DCX by human brain tumors. PMID: 16195916 Doublecortin can be regarded as specific neuronal marker only in normal developing brain, but lacks specificity in glioneuronal and glial tumours and other non-neuronal human tissues where it is expressed in a wide variety of tumours and tissues. PMID: 16520969 X-ray structure of a mutant of N-DCX, in which the C-terminal fragment (residues 139-147) unexpectedly shows an altered, "open" conformation PMID: 16835924 Our data in the mouse, identifying roles for Dcx in hippocampal and corpus callosal development, might suggest intrinsic roles for human DCX in the development of these structures. PMID: 17111359 Doublecortin (DCX) is one of the three genes found from Affymetrix gene chip analysis related to glioma patient survival. PMID: 17178868 MLPA uncovers large genomic deletions of the DCX gene in a subset of patients with SBH in whom no mutations are found after gene sequencing. Deletions of DCX are an underascertained cause of SBH. PMID: 17283321 Results demonstrate that DCX can be used as a marker to distinguish articular chondrocytes from other chondrocytes and to evaluate the quality of tissue engineered or regenerated cartilage in terms of their "articular" or "non-articular" nature. PMID: 17897623 Doublecortin is applicable for the detection of individual infiltrating glioma cells when combined with other markers. PMID: 18415660 X-linked lissencephaly patients with mutations in the C-DC domain tended to have a less severe lissencephaly (grade 4-5 in 58.3%) compared with those in the N-DC domain (grade 4-5 in 36.3%. PMID: 18685874 intragenic deletions and duplications of the DCX gene account for a significant number of patients with isolated lissencephaly sequence and subcortical band heterotopia, where no molecular defect had previously been identified. PMID: 19050731 Doublecortin (DCX) upregulation, correlates with better neurologic outcome in children following traumatic head injury. PMID: 19221293

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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