Recombinant Human Neuroepithelial Cell-Transforming Gene 1 Protein (NET1) Protein (His-SUMO)

Name: Recombinant Human Neuroepithelial Cell-Transforming Gene 1 Protein (NET1) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-09949P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Neuroepithelial Cell-Transforming Gene 1 Protein (NET1) Protein (His-SUMO) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	Q7Z628
Target Symbol	NET1
Synonyms	ARHG8_HUMAN; ARHGEF8; GLYT2 ; Guanine nucleotide regulatory protein oncogene; MGC114559 ; MGC127767 ; MGC53602 ; mNET1; Net1; NET1A; Neuroepithelial cell transforming gene 1; Neuroepithelial cell-transforming gene 1 protein; p65 Net1 proto oncogene; Proto-oncogene p65 Net1; Rho guanine nucleotide exchange factor 8; Rho guanine nucleotide exchange factor GEF 8
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	MVAHDETGGLLPIKRTIRVLDVNNQSFREQEEPSNKRVRPLARVTSLANLISPVRNGAVRRFGQTIQSFTLRGDHRSPASAQKFSSRSTVPTPAKRRSSALWSEMLDITMKESLTTREIRRQEAIYEMSRGEQDLIEDLKLARKAYHDPMLKLSIMSEEELTHIFGDLDSYIPLHEDLLTRIGEATKPDGTVEQIGHILVSWLPRLNAYRGYCSNQLAAKALLDQKKQDPRVQDFLQRCLESPFSRKLDLWSFLDIPRSRLVKYPLLLKEILKHTPKEHPDVQLLEDAILIIQGVLSDINLKKGESECQYYIDKLEYLDEKQRDPRIEASKVLLCHGELRSKSGHKLYIFLFQDILVLTRPVTRNERHSYQVYRQPIPVQELVLEDLQDGDVRMGGSFRGAFSNSEKAKNIFRIRFHDPSPAQSHTLQANDVFHKQQWFNCIRAAIAPFQSAGSPPELQGLPELHEECEGNHPSARKLTAQRRASTVSSVTQVEVDENAYRCGSGMQMAEDSKSLKTHQTQPGIRRARDKALSGGKRKETLV
Expression Range	1-542aa
Protein Length	Full Length of Isoform 2
Mol. Weight	77.9kDa
Research Area	Signal Transduction
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.
Subcellular Location	Cytoplasm. Nucleus.
Database References	HGNC: 14592 OMIM: 606450 KEGG: hsa:10276 STRING: 9606.ENSP00000347134 UniGene: PMID: 30301500 Study found NET1 as a direct target for miR-22 to promote chemoresistance of bladder cancer cells. PMID: 29620229 We suggest that the oncogenic properties of CML cells are probably due to deregulated expression of NET1 as a result of altered expression of miR-22. PMID: 25041463 Net1 promotes the angiogenesis in cervical squamous cell carcinomas and siRNA targeting of Net1 can effectively reduce angiogenesis and inhibit tumor growth via VEGF signaling. PMID: 28511963 AMPK phosphorylation of the RHOA guanine nucleotide exchange factor NET1A inhibits extracellular matrix degradation, an early step in cell invasion PMID: 26456332 Net1 mRNA and protein levels in non-small cell lung cancer tissues were significantly elevated compared with those in their corresponding nontumor tissues. PMID: 26459749 Simultaneous silencing of NET-1 and survivin using one-chain-double-target siRNA thus provides an advantageous alternative in the development of therapeutics for skin squamous cell carcinoma . PMID: 26080853 These data indicate that Net1A acetylation regulates its subcellular localization to impact on RhoA activity and actin cytoskeletal organization. PMID: 25588829 NET1 was associated with comorbidity of oppositional defiant disorder and symptoms in Attention-deficit/hyperactivity disorder probands PMID: 24942521 Net1A plays a plays a previously unrecognized, Rho GTPase-independent role in controlling ATM activity and downstream signaling after DNA damage to impact cell survival. PMID: 25486363 High expression of neuroepithelial transforming gene 1 was associated with hepatocellular carcinoma. PMID: 25113254 The overexpression of NET-1 in tumor cells may be closely related to the malignant phenotype of skin squamous cell carcinoma including proliferation, invasion and tumor growth. PMID: 24196235 NET1 expression is elevated in OAC and its pre-malignant phenotype, Barrett's oesophagus. NET1 promotes OAC cell invasion and proliferation. PMID: 23945136 NET-1 and VEGF silencing play a key role in inhibiting hepatocellular cell proliferation, promoting apoptosis, and reducing angiogenesis. PMID: 23636606 Results identify Net1 as a novel regulator of mitosis and indicate that altered expression of Net1, as occurs in human cancers, may adversely affect genomic stability. PMID: 23864709 Data suggests that NET1 may be of prognostic significance in the clinical management of gastric adenocarcinoma PMID: 24375299 A combination of NET1 (rs2242447) and SNAP-25 (rs3746544) is a risk factor for ADHD. PMID: 23872233 A previously unrecognized role for the Net1A isoform in controlling FAK activation during planar cell movement. PMID: 23689132 Rac1 activation causes relocalization of Net1 isoforms outside the nucleus and stimulates Net1A catalytic activity. PMID: 23184663 Net -1 frameshifting on a noncanonical sequence in a herpes simplex virus drug-resistant mutant is stimulated by nonstop mRNA. PMID: 22927407 Net1 is a CARMA-interacting molecule and brings important information on the molecular mechanisms that control NF-kappaB transcriptional activity PMID: 22343628 A mechanism involving the nuclear GEFs Ect2 and Net1 for activating RhoB after genotoxic stress, thereby facilitating cell death after treatment with DNA damaging agents. PMID: 21373644 Existence of a functional Net1/RhoA signaling pathway within the nucleus of the cell and this implicates them in the DNA damage response. PMID: 21390328 NET1 plays an role in gastric cancer cell migration and invasion. The gene expression profile further elucidates the molecular mechanisms underpinning NET1-mediated aggressive GC cell behaviour. PMID: 21284875 RNAi targeting NET-1 gene effectively down-regulates the expression of NET-1 in A431 cells, leading to an inhibition of proliferation, migration and infiltration. PMID: 20078975 Smad3 regulates RhoA activation and cytoskeletal reorganization by controlling NET1 in TGF-beta1-induced ARPE-19 cells. PMID: 20547485 NET-1 protein is expressed in cytoplasm of HCC cells as irregular granules near Golgi apparatus, and may promote the uncontrolled proliferation and abnormal differentiation in HCC cells. PMID: 18478931 NET1 has roles in stimulating cytoskeletal reorganization and in transforming cells PMID: 15611121 PAK1 negatively regulates the activity of NET1 PMID: 15684429 The putative role for NET1 genes may provide important targets for intervention in Gastric adenocarcinoma, evidenced by their role in promoting invasion and proliferation, key phenotypic hallmarks of cancer cells. PMID: 16552434 These results suggest that the interaction between Net1 and Dlg1 may contribute to the mechanism of Net1-mediated transformation. PMID: 17938206 study concludes expression of NET-1 may relate to proliferation, metastasis & clinic stages of hepatocellular carcinoma (HCC); expression in HCC tissues may positively correlate to TMN stages; expression in HCC tissues was positively correlated with PCNA PMID: 18214716 data highlight the importance of Net1 in controlling RhoA and p38 MAPK mediated cell survival in cells exposed to DNA damaging agents PMID: 18509476 the importance of NET1 as a driver of tumour cell invasion PMID: 18827818 NET1 and integrin alpha6beta4 coexpression is independently associated with the development of distant metastasis in breast cancer. PMID: 19124484 Net1 requires interaction with PDZ domain proteins, such as Dlg1, to protect it from proteasome-mediated degradation and to maximally stimulate RhoA and that this interaction is regulated by cell-cell contact. PMID: 19586902

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.