Recombinant Human N (DDAH2) Protein (His-GST)

Name: Recombinant Human N (DDAH2) Protein (His-GST)
Brand: Beta LifeScience
SKU: BLC-01122P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Human N (DDAH2) Protein (His-GST) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	O95865
Target Symbol	DDAH2
Synonyms	(DDAH-2)(Dimethylarginine dimethylaminohydrolase 2)(DDAHII)(Dimethylargininase-2)(Protein G6a)(S-phase protein)
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His-GST
Target Protein Sequence	MGTPGEGLGRCSHALIRGVPESLASGEGAGAGLPALDLAKAQREHGVLGGKLRQRLGLQLLELPPEESLPLGPLLGDTAVIQGDTALITRPWSPARRPEVDGVRKALQDLGLRIVEIGDENATLDGTDVLFTGREFFVGLSKWTNHRGAEIVADTFRDFAVSTVPVSGPSHLRGLCGMGGPRTVVAGSSDAAQKAVRAMAVLTDHPYASLTLPDDAAADCLFLRPGLPGVPPFLLHRGGGDLPNSQEALQKLSDVTLVPVSCSELEKAGAGLSSLCLVLSTRPHS
Expression Range	1-285aa
Protein Length	Full Length
Mol. Weight	61.2 kDa
Research Area	Neuroscience
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
Subcellular Location	Cytoplasm. Mitochondrion. Note=Translocates from cytosol to mitochondrion upon IL-1beta stimulation in chondrocytes.
Protein Families	DDAH family
Database References	HGNC: 2716 OMIM: 604744 KEGG: hsa:23564 STRING: 9606.ENSP00000364943 UniGene: PMID: 28590543 The main finding from this study was that it demonstrated that the C-allele of rs3087894 in DDAH1 is a risk factor for hypertension in the Kazakh group but a protective factor in the Uygur group. In addition, we did not find any genotype of DDAH1 and DDAH2 associated with hypertension in the Han group. PMID: 26786611 Exogenous human DDAH2 gene promotes differentiation of rabbit bone marrow-derived endothelial progenitor cells into mature endothelial cells. PMID: 28150318 expression of DDAH2 is associated with invasiveness of lung adenocarcinoma via tumor angiogenesis PMID: 26515557 Inhibiting the expression of DDAH1, but not DDAH2, resulted in a significant increase in the sensitivity of the EVT cell line SGHPL-4 to tumour necrosis factor related apoptosis inducing ligand (TRAIL) induced apoptosis PMID: 26082478 increased ADMA levels in rheumatoid arthritis do not appear to relate to DDAH genetic polymorphisms PMID: 25194333 The percentage of senescent endothelial progenitor cells increased while the expression of DDAH2 decreased concomitantly with an increase in the plasma levels of asymmetric dimethylarginine in patients with type 2 diabetes mellitus. PMID: 25701782 DDAH-1 is a specific molecular target for portal pressure reduction, through actions on ADMA-mediated regulation of eNOS activity. PMID: 25152204 Our results suggest that the rs805304 C allele of the DDAH gene was associated with decreased risk of myocardial infarction and decreased risk of obesity. PMID: 25236572 Homocysteine disrupts EPCs function via inducing the hypermethylation of DDAH2 promoter, suggesting a key role of epigenetic mechanism in the progression of atherosclerosis PMID: 24934151 -476 to -469 of the DDAH2 promoter was a NF-kB responsive element and is important for the transactivation of DDAH2. PMID: 24928011 The rs9267551 functional variant of the DDAH2 gene is associated with chronic kidney disease with carriers of the C allele having a lower risk of renal dysfunction independently from several confounders PMID: 24125425 Our approaches revealed signature candidates of differentially hypermethylated genes of DDAH2 and DUSP1 which can be further developed as potential biomarkers for OSCC as diagnostic, prognostic and therapeutic targets in the future. PMID: 24155659 Enhancing pulmonary DDAH II activity attenuates LPS-mediated lung leak in acute lung injury. PMID: 24134589 There is a significant difference in distribution of DDAH2 gene polymorphism among hemodialysis patients compared to healthy individuals. PMID: 23770786 DDAH2-1151 A/C polymorphism is associated with Chronic renal impairment in type 2 diabetes. PMID: 23129820 Glucose-stimulated insulin secretion is increased in Ddah2-transgenic pancreatic islets by 33%, compared to its levels in wild-type mice. PMID: 23430976 Suppression of DDAH2 expression is a culprit for homocysteine-induced impairments of DDAH/ADMA/NOS/NO pathway in endothelial cells. PMID: 23171931 Found that SNP rs2272592 in DDAH2 is associated with type 2 diabetes but SNP rs805304 in DDAH2 is not. DDAH2 SNP rs2272592 AG+GG genotypes are associated with genetic susceptibility to type 2 diabetes in Korean population. PMID: 22579530 No association was observed between the DDAH2 polymorphisms at rs805305 and rs2272592 and coronary heart disease. PMID: 22923027 A functional polymorphism of the DDAH2 gene may confer increased risk for type 2 diabetes by affecting insulin sensitivity PMID: 22558392 The -449G single nucleotide polymorphism within the DDAH2 gene was associated with both decreased plasma asymmetric dimethylarginine and an increased likelihood of presenting with "cold" shock in pediatric sepsis. PMID: 22428028 DDAH-2 could play an important role in IL-1beta-induced NO production and in osteoarthritis pathogenesis. PMID: 21898353 Low expression of DDAH-2 in placenta and increased serum asymmetric dimethylarginine level might confer susceptibility to preeclampsia. PMID: 19570459 a possible association between A allele / AA genotype for DDAH2 SNP1 (-1151 C/A, rs805304) and G allele / GG genotype for SNP2 (-449 C/G, rs805305) with CVD in male 35-50 year-old Egyptian patients was observed. PMID: 21677405 DDAH1 and DDAH2 polymorphisms are strongly and additively associated with serum ADMA concentrations in individuals with type 2 diabetes PMID: 20209122 Single nucleotide polymorphisms in the DDAH2 gene are associated with blood pressure levels, prevalence of hypertension, and left ventricular mass and function in the general population. PMID: 19666123 In cultured endothelial cells, heterologously expressed human DDAH II was S-nitrosylated after cytokine induced expression of the inducible NOS isoforms. PMID: 12370443 DDAH2 expression in endothelial cells is altered by genetic variation in a basal promoter element PMID: 14550280 messenger RNA and protein were demonstrated in first trimester placental tissue, primary extravillous trophoblasts and extravillous trophoblast-derived cell lines. PMID: 16920729 Allelic variation for a polymorphism in the DDAH II gene may influence asymmetrical dimethyl arginine concentrations, hence the severity of organ failure in septic shock patients. PMID: 17002794 The purpose of this study was to investigate whether there is any association between preeclampsia and eNOS, DDAH, and VEGF gene polymorphisms.Polymorphisms in eNOS, DDAH, and VEGF gene do not seem to be risk factors for preeclampsia. PMID: 18251679 Lysophosphatidylcholine (LPC) impairs DDAH/ADMA/NOS/NO pathway, and DDAH2 gene transfer could improve the LPC-elicited impairments in endothelial cells. PMID: 18342305 results suggest that the DDAH2 common variant may play a protective role in the development of Intracerebral Hemorrhage, implicating that the DDAH2/ADMA pathway may act as a critical regulator of cerebral small-vessel disorders PMID: 19250061 DDAH2 mRNA expression is inversely associated with some cardiovascular risk-related features. PMID: 19822957

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.