Recombinant Human Muscarinic Acetylcholine Receptor M1 (CHRM1) Protein (His)

Name: Recombinant Human Muscarinic Acetylcholine Receptor M1 (CHRM1) Protein (His)
Brand: Beta LifeScience
SKU: BLC-00813P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Muscarinic Acetylcholine Receptor M1 (CHRM1) Protein (His) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	P11229
Target Symbol	CHRM1
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-10His
Target Protein Sequence	RIYRETENRARELAALQGSETPGKGGGSSSSSERSQPGAEGSPETPPGRCCRCCRAPRLLQAYSWKEEEEEDEGSMESLTSSEGEEPGSEVVIKMPMVDPEAQAPTKQPPRSSPNTVKRPTKKGRDRAGKGQKPRGKEQLAKRKTFSLVKEKKAART
Expression Range	210-366aa
Protein Length	Partial
Mol. Weight	20.8 kDa
Research Area	Neuroscience
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
Subcellular Location	Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
Protein Families	G-protein coupled receptor 1 family, Muscarinic acetylcholine receptor subfamily, CHRM1 sub-subfamily
Database References	HGNC: 1950 OMIM: 118510 KEGG: hsa:1128 STRING: 9606.ENSP00000306490 UniGene: PMID: 27490167 he discovery of a receptor homolog of human mAChR1 on Acanthamoeba with future studies planned to show its expression and binding to cholinergic agonist and antagonist would help clarify its role in the biology of this protist pathogen. PMID: 27601178 Receptor Species-dependent Desensitization Controls KCNQ1/KCNE1 K+ Channels as Downstream Effectors of Gq Protein-coupled Receptors.( PMID: 27834678 There were no significant associations between CHRM1 SNPs and autonomic nervous system activity in patients with schizophrenia on high-dose antipsychotics. PMID: 27923235 changes in membrane cholesterol concentration differentially impact preferential and non-preferential M1 and M3 receptor signaling PMID: 24821386 The rs2067477 muscarinic M1 receptor genotype is associated with grey matter volume in schizophrenia. PMID: 26481978 crystal structures of the M1 and M4 muscarinic receptors bound to the inverse agonist, tiotropium PMID: 26958838 this study highlights how the properties of affinity and cooperativity can be differentially modified on a common structural scaffold and identifies molecular features that can be exploited to tailor the development of M1 mAChR-targeting PAMs. PMID: 25326383 CHRM2 but not CHRM1 or CHRM3 polymorphisms are associated with asthma susceptibility in Mexican patients. PMID: 24430298 the levels were comparable for complexes containing GluR2, GluR3 and GluR4 as well as 5-HT1A. Moreover, the levels of complexes containing muscarinic AChR M1, NR1 and GluR1 were significantly increased in male patients with AD. PMID: 24292102 We show that BQCA potentiates agonist-induced beta-arrestin recruitment to M1 mAChRs. PMID: 24753247 In patients with schizophrenia, both cell body staining and elevated M1 muscarinic receptor reactivity correlated with higher symptom scores. PMID: 24636402 demonstrate that activation of M1 muscarinic acetylcholine receptor augments the restitution of epithelial barrier function in T84 cell monolayers after ethanol-induced epithelial injury, via ERK-dependent phosphorylation of focal adhesion kinase PMID: 24365239 Mutation of amino acid residues that form the orthosteric binding pocket caused a loss of carbachol response that could be rescued by BQCA. PMID: 24443568 Results show that downregulation of cholinergic receptor M1 by histone H3K9me3 impairs calcium signally and contributes to the pathogenesis of Huntington's disease. PMID: 23455440 Decreased muscarinic M1 receptor radioligand binding is found in the cerebral cortex of schizophrenic patients. PMID: 22338582 M1-mAChRs function at both surface and intracellular sites in telencephalon neurons including the hippocampus PMID: 23678982 The fluorescent compounds inherit bitopic properties from a linker-guided positioning of their Bodipy moiety within the M1 allosteric vestibule. PMID: 23604140 Autoantibodies purified from the sera of breast cancer patients activate muscarinic acetylcholine receptors in tumor cells. PMID: 23460876 A breakdown in gene promoter methylation control of CHRM1 expression contributes to the global pathophysiology of schizophrenia. PMID: 23423139 These results suggest a complex antagonistic interplay between G(q)-activated PKC and Gbetagamma in regulation of L-VDCC, in which multiple cytosolic segments of alpha(1C) are involved. PMID: 22990911 Collectively, the results of this study demonstrated that SOD1 could activate a transductional pathway through the involvement of M1 muscarinic receptor. PMID: 23147108 These data indicate that EGFR and PKC are involved in mAChR-mediated activation of ERK1/2 and RSK and the subsequent proliferation of SNU-407 colon cancer cells. PMID: 22865467 CHRM1 sequence is associated with alterations in some aspects of executive function. Post-mortem study indicates this is not simply due to altered expression at the mRNA level, suggesting sequence alteration may affect the functionality of the CHRM1. PMID: 22391213 Here we found that M1 Muscarinic acetylcholine receptor (M1 mAChR), an important G protein-coupled receptor involved in cholinergic neuronal activity, can interact with BACE1 and mediate its proteosomal degradation. PMID: 22450048 The binding mode of MT7 and human muscarinic acetylcholine receptor 1 consists of three interaction regions in five residue interaction clusters. PMID: 22571431 Binding properties of human muscarinic M1 receptor in stably transfected Chinese hamster ovary cells is significantly enhanced after supplementation, probably by facilitating receptor-mediated G protein activation. PMID: 22146060 docking calculations structurally support the high affinity and selectivity of the MT7-human M1 muscarinic receptor interaction and highlight the atypical mode of interaction of this allosteric ligand on its G protein-coupled receptor target. PMID: 21685390 analysis of the long-term effects of xanomeline on the M(1) muscarinic acetylcholine receptor PMID: 21203415 Distribution of M1 receptors in human colon. PMID: 20146726 M1 receptor plays an important role in the maintenance of function of retinal pigment epithelium. PMID: 17415969 Data show that two-color TIRFM established the dynamic nature of dimer formation with M(1) receptors undergoing interconversion between monomers and dimers on the timescale of seconds. PMID: 20133736 increased inattention scores (Conners' teacher-rating scale) and lower l-MR associated with increased score for oppositional-defiant disorder. PMID: 19863190 This study demonistred that in subgroup of subjects with schizophrenia decreased cortical muscarinic acetylcholine receptor M1. PMID: 18317461 Muscarinic cholinoceptor activation by pilocarpine triggers apoptosis in human skin fibroblast cells. PMID: 19927300 Fifteen single nucleotide polymorphisms (SNPs) of CHRM1 have been discovered, 9 of which are located in the coding region of the receptor; of these, 8 represent synonymous SNPs, indicating that CHRM1 is highly conserved in humans. PMID: 12049494 28% decrease in post-mortem levels of steady-state cortical muscarinic 1 receptor cDNA in schizophrenia patient samples relative to controls PMID: 12707929 role of M1 and M2 muscarinic receptors expressed by human L cells in the control of GLP-1 secretion. PMID: 12810581 In conclusion, autoantibodies to CHRM1 were detected in a large number of CFS patients and were related to CFS symptoms. PMID: 12851722 M1- and M3- but not M2- or M4-AchR signals activate HIF-1 by both stabilization and synthesis of HIF-1alpha and by inducing the transcriptional activity of HIF-1alpha. PMID: 15280370 mRNA of muscarinic acetylcholine receptor-1 is expressed in human scleral fibroblasts. PMID: 16318751 All three of the Kir2 channels are similarly inhibited by m1 muscarinic receptor stimulation through calcium-dependent activation of the small GTPase Rho. PMID: 16328454 Increased phosphorylation of M1 and M2 mAChRs underlies sequestration of these receptors after transient hypoxia. Distinct pathways involving CK1alpha and GRK2 mediated sequestration of M1 and M2 mAChRs after transient hypoxic-induced oxidative stress. PMID: 16336219 Data demonstrate that M(1), M(2), and M(3) muscarinic acetylcholine receptors (mAChR)can form homo- and heterodimers in living cells, and suggest that heterodimerization plays a role in the mechanism of mAChR long term regulation. PMID: 16368694 Data show that intracellular zinc uptake in SK-SH-SY5Y cells is controlled by M1-mAChR mediated signalling pathways and that zinc may act as a cofactor for transcriptional regulation of zinc finger genes such as PNUTS. PMID: 16406470 MT7 toxin interacts with hM1 receptor at a specific allosteric site, which may partially overlap those identified previously for "classic" or "atypical" allosteric agents. PMID: 16439611 Association of the nicotinic acetylcholine receptor beta1 subunit (CHRNB1) and M1 muscarinic acetylcholine receptor (CHRM1) with vulnerability for nicotine dependence. PMID: 16874522 mAChR subtypes (m1 to m5) presents in human scleral fibroblasts at both mRNA and protein levels. PMID: 16877267 This study provides the structural insight for improved understanding of M1 receptor and its binding sites. PMID: 16902941 Gene Coding the human cholinergic receptor muscarinic-1 is an important susceptibility locus for asthma at chromosome 11q13. PMID: 16931638

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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