Recombinant Human/Mouse/Rat Activin A Protein , Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0047UL

Recombinant Human/Mouse/Rat Activin A Protein , Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0047UL
/
Size

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Product Overview

Product Name Recombinant Human/Mouse/Rat Activin A Protein
Target Symbol INHBA
Alternative Names Inhibin Subunit Beta A, Erythroid Differentiation Factor, Inhibin Beta A Chain, Activin Beta-A Chain, Follicle-Stimulating Hormone-Releasing Protein, FSH-Releasing Protein, INHBA, EFD, FRP
Accession Number P08476
Expression System E. coli.
Molecular Weight 13 kDa (monomer, predicted)
Sequence Gly311-Ser426
Purity >98% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining
Activity Determined by a cell proliferation assay using mouse L-929 cells treated with Actinomycin D. The ED50 for this effect is typically <40 pg/mL.
Formulation Lyophilized from sterile PBS with Trehalose, pH 7.4
Endotoxin Level <0.001 EU per 1 μg of the protein as determined by the LAL method
Shipping Shipped at ambient temperature.
Stability & Storage 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.
Reconstitution Reconstitute at 100 ug/mL in sterile PBS.
Target Function Activin A plays critical roles in various physiological processes, including development, reproduction, immune function, tissue repair and tissue homeostasis. During embryonic development, Activin A is involved in patterning and differentiation of various tissues and organs. It helps regulate processes such as mesoderm induction, neural differentiation, and organogenesis. Activin A is involved in the regulation of reproductive processes such as follicle development in the ovaries, spermatogenesis in the testes, and embryonic implantation in the uterus. It also plays a role in the secretion of hormones such as follicle-stimulating hormone (FSH) and luteinizing hormone (LH) from the pituitary gland. Activin A has immunomodulatory effects, influencing the function of immune cells such as macrophages, T cells, and B cells. Activin A participates in the regulation of bone metabolism by influencing the activity of osteoblasts and osteoclasts. During tissue repair, Activin A is upregulated and promotes growth of keratinocytes and stromal cells. Activin A is a homodimer consisting of two betaA subunits. Activin A signaling takes places through binding to a Type II receptor (Activin RIIA/ACVR2A or Activin RIIB/ACVR2B) followed by heterodimerization with a Type I receptor unit (Activin RIB/ACVR1B/ALK-4 or Activin RIC/ACVR1C/ALK-7). Regulation of Activin A occurs by binding to inhibitory proteins (Follistatin and FLRG) or membrane-bound decoy receptors (BAMBI and Cripto). Activin A is used in cell culture to maintain pluripotency of human embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs) and to differentiate human ESCs into endoderm. Similarly, Activin A is used for the maintenance and growth of 3-D organoids.
Tissue Specificity Activin A expression has been reported in adipose tissue, ovaries, testes, uterus, pituitary gland, placenta, prostate, lung, kidney, skeletal muscle, and cardiac muscle.
Cellular Localization Secreted protein
Involvement In Disease Dysregulation of Activin A has been associated with osteoporosis, rheumatoid arthritis (RA), inflammatory bowel disease (IBD), asthma, obesity, type 2 diabetes, atherosclerosis, and cancers (prostate, breast, ovarian, and colorectal).

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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