Recombinant Human MMP-1 Protein (C-6His)

Name: Recombinant Human MMP-1 Protein (C-6His)
Brand: Beta LifeScience
SKU: BL-1327NP
Availability: InStock

BL-1327NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

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Product Overview

Description	Recombinant Human Matrix Metalloproteinase-1 is produced by our Mammalian expression system and the target gene encoding Phe20-Asn469 is expressed with a 6His tag at the C-terminus.The proenzyme needs to be activated by APMA for an activated form.
Accession	P03956
Synonym	Interstitial Collagenase; Fibroblast Collagenase; Matrix Metalloproteinase-1; MMP-1; MMP1; CLG
Gene Background	Matrix Metalloproteinase-1 (MMP-1) is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. MMP1 contains several distinct domains: a prodomain that is cleaved upon activation, a catalytic domain containing the zinc binding site, a short hinge region, and a carboxyl terminal (hemopexin like) domain. MMP-1 can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin, α1 antitrypsin, myelin basic protein, L-Selectin, pro-TNF, IL1, IGFBP3, IGFBP5, pro-MMP2, and pro-MMP9. A significant role of MMP1 is the degradation of fibrillar collagens in extracellular matrix remodeling, characterized by the cleavage of the interstitial collagen triple helix into 3/4, 1/4 fragments. MMP1 may also be involved in enzyme cascades, cytokine regulation and cell surface molecule modulation.
Molecular Mass	52.88 KDa
Apmol Mass	56 KDa, reducing conditions
Formulation	Supplied as a 0.2 μm filtered solution of 20mM MES, 150mM NaCl, 2mM CaCl2, 1mM DTT, 0.05%Brij35, 10% Glycerol, pH 5.0.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution
Storage	Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Shipping	The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
Subcellular Location	Secreted, extracellular space, extracellular matrix.
Protein Families	Peptidase M10A family
Database References	HGNC: 7155 OMIM: 120353 KEGG: hsa:4312 STRING: 9606.ENSP00000322788 UniGene: PMID: 30117779 rs1799750 in MMP1 might be a risk factor for rheumatic heart disease in a Han population in Southern China, and individuals carrying the 2G/2G genotype are likely more susceptible to rheumatic heart disease. PMID: 29458338 RHCG Suppresses Tumorigenicity and Metastasis in Esophageal Squamous Cell Carcinoma via Inhibiting NF-kappaB Signaling and MMP1 Expression. PMID: 29290801 Malignant extracellular vesicles carrying MMP1 mRNA facilitate peritoneal dissemination in ovarian cancer. PMID: 28262727 Data suggest that matrix metalloproteinase-1 and neutrophil elastase may be important but heretofore unrecognized players in stimulating uterine contractions at the time of labor, and they may explain why indomethacin delays, but does not prevent, preterm labor because indomethacin inhibits the prostaglandin component but not the protease component of labor. PMID: 28954603 the present study demonstrated that scopoletin inhibited MMP1 and proinflammatory cytokine expression by inhibiting p38 MAPK phosphorylation. PMID: 30015831 Matrix metalloproteinase 1(MMP1), which was downregulated in myelodysplastic syndromes (MDS)-mesenchymal stromal cells (MSCs), was identified as an inhibitory factor of MDS cell proliferation. PMID: 28262842 study to explore the relationship between 2 polymorphisms (MMP-1-755 T/G [rs498186] and MMP-3 A/C [rs632478]) and disc degeneration; no significant association of the MMP-1 polymorphism with disc degeneration was found PMID: 28497435 Expression of GSDMC is increased by UV irradiation in human skin keratinocytes. GSDMC induction contributes to the increase of MMP-1 expression specifically via activation of ERK and JNK pathways. PMID: 29428815 our results suggest that the typical 1G/2G MMP-1 polymorphic site did not play a direct determinant role in breast cancer among Taiwanese women. PMID: 29695550 The maternal and fetal 1G/1G MMP1 gene polymorphisms may be strong genetic markers of preeclampsia, occurring either individually or together. PMID: 29670668 The current study indicated that MMP1 has a key role in the regulation of cervical tumor growth and lymph node metastasis via epithelial mesenchymal transition to a certain extent PMID: 29436615 2G allele of MMP-1, C allele of MMP-2 and 5A/6A genotype of MMP-3 are associated with susceptibility and disease progression of type 2 diabetic nephropathy PMID: 29883760 this study provides insights into the development of a possible alternative therapy manipulating MMP-1 and the mTOR signaling pathway in Epidermal growth factor receptor-tyrosine kinase inhibitor-resistant lung adenocarcinoma. PMID: 29463039 Specific silencing that targeted AMPK resulted in the enhancement of mobility and contractility and in the production of MMP-1 and MMP-2. PMID: 29122080 MMP-1 genotype may accelerate the development of HIV-associated neurocognitive disorder (HAND) whereas MMP3 -1612 5A5A genotype may reduce the risk of pathogenesis of HAND. PMID: 29358561 Authors observed a significant increase in the immunoexpression of collagen I and III in patients with DCM and a significant reduction in the immunoexpression of MMP-1 compared with the control group. Also, the collagen I and TIMP-1 expression indicated a positive linear correlation and respectively a negative linear relationship with collagen III and MMP-1. PMID: 29250654 findings suggest that the polymorphic genotypes at MMP-1 promoter -1607 may play a major role in determining personal cancer susceptibility for prostate cancer in Taiwan PMID: 29970511 No significant association was found between MMP-1 (-16071G/2G, -519A/G) variants and obesity. PMID: 29317790 Reduced expression of types I and III collagen and TIMP-1 as well as the increased expression of MMP-1 and MMP-8 in the anterior vaginal wall tissues play important roles in the onset of pelvic organ prolapse. PMID: 29263043 The results of the present study suggested that miR93 regulated MMP1 and collagen I expression in fibroblasts via calpain2. miR93 mediated collagen expression in stress urinary incontinence via calpain2. PMID: 29115452 This study demonstrates that matrix remodeling caused by matrix metalloproteinase (MMP)-1 regulates phenotypic activities and modulates radiosensitivity of hepatocarcinoma cells exclusively in a 3D matrix. In response to MMP-1, liver cancer cells undergo active proliferation, downregulation of E-cadherin, and the loss of detoxification capacity. PMID: 28683186 Results suggested that miR-361 directly inhibited MMP-1 expression by binding to its 3'-UTR suppressing breast cancer cells invasion and metastasis. PMID: 29132384 Review/Meta-analysis: MMP-1 -1607 1G/2G and MMP-12 -82 A/G gene polymorphisms are associated with ischemic stroke risk. PMID: 28958661 Results show that head and neck squamous cell carcinoma tumors with low P120CTN and PI3K pathway mutations have higher levels of MMP1 compared to tumors with high P120CTN and no PI3K pathway mutations demonstrating that P120CTN downregulation and PIK3CA mutations promote MMP1-driven invasion. PMID: 28637905 MMP-1 (and MMP-2 and MMP-3) are independently associated with markers of arterial stiffening in patients with type 1 diabetes. PMID: 29070037 There was no statistically significant difference in the distribution of the MMP1-1607dupG (rs1799750) polymorphism between patients with unstable angina and the control group. PMID: 29044936 PTEN inhibits replicative senescence-induced MMP-1 expression by regulating NOX4-mediated reactive oxygen species in human dermal fibroblasts. PMID: 28557373 None of the studied polymorphisms showed statistically significant association with the risk of varicose veins of the lower extremities. PMID: 28944430 Matrix metalloproteinase 1 rs1799750 polymorphism was significantly associated with nasopharyngeal carcinoma risk in the subgroup analysis by smoking, a marginally significant association was found in non-smokers. PMID: 29096757 Studied the association between the matrix metalloproteinase-1 (MMP-1) 1607 1G/2G polymorphism and susceptibility to spinal tuberculosis in Southern Chinese Han population. PMID: 28129430 MMP1 was consistently and significantly upregulated in the induced pluripotent stem cells-derived endothelia from autosomal dominant polycystic kidney disease patients with intracranial aneurysms compared with the patients without intracranial aneurysms in multiple assays, indicating that high serum MMP1 levels may be a novel risk factor. PMID: 27418197 rs1144393 in the MMP1 promoter region can be a predictor of grade >/= 2 RILI in lung cancer patients treated with thoracic radiation. PMID: 27659527 The present results suggested that Icariin may have a chondroprotective effect, exerted through the inhibition of MMP1, MMP3 and MMP13 via MAPK pathways. Therefore, Icariin may have potential as a novel therapeutic strategy for the treatment of osteoarthritis. PMID: 28447732 proteolysis of collagen-rich natural extracellular matrix (ECM), performed uniquely by individual homologous proteases, leads to distinct events that eventually affect overall ECM morphology, viscoelastic properties, and molecular composition. PMID: 27630193 SAA1 can be a potential mediator for UV-induced MMP-1 expression in human skin. PMID: 26900010 collagen II-activated phosphorylated-DDR2 induces CYR61 through activation of transcription factor activator protein 1 (AP-1). The elevated CYR61, in turn, accelerates MMP1 production via ETS1 (ETS proto-oncogene 1). PMID: 27653023 M. tuberculosis infection caused enhanced MMP-1, -9, and miR-223 expression, with inhibited BMAL1 expression. MiR-223 modulated BMAL1 expression via the direct binding of BMAL1 3'-UTR. PMID: 28543681 20(S)-PPD treatment modulated MMP-1 mRNA expression and the transcriptional activity of activator protein (AP)-1, a major transcription factor of MMP-1. PMID: 28379603 This is the first study to indicate that the genotypes of MMP1 promoter -1607, do not significantly confer susceptibility to lung cancer in a Taiwanese population. PMID: 29277780 Matrix metalloproteinase-1, -8, -9 and the risk of cardiovascular complications in patients with CHD before and after myocardial revascularization. PMID: 29215835 Results show that the expression level of MMP1 and TIMP1 is under the regulation of HAS2 leading to migration, invasion and EMT of oral cancer cells. PMID: 27884164 DDC up-regulated MMP-1 in LX-2 cells through the Akt and ERK/miR-222/ETS-1 pathways. PMID: 27412967 Meta-analysis of existing data showed that rs1799750 may affect individual susceptibility to glaucoma. PMID: 27428613 results suggest that MMP1-1607 1G/2G, MMP3-1171 5A/6A and MMP9-1562 C/T gene polymorphisms have synergistic effect on breast cancer. The interactions of MMPs clinical risk factors such as lymph node involvement has shown a strong correlation and might influence the 5-years survival rate, suggesting their potential role in the breast carcinogenesis PMID: 28961241 The MMP-1 -1607 (2G/1G) and -519 (A/G) polymorphisms may contribute to the onset of osteomyelitis. The 2G/2G genotype carriers had 1.605 times risk of developing osteomyelitis. In addition, frequency of -519GG genotype was obviously higher in case group than that in control group, and GG genotype related to an increased risk of osteomyelitis. Whereas, the -519G allele may be a susceptible factor for osteomyelitis. PMID: 29049163 No differences in the distribution of the MMP-1 variants were found among Tunisian chronic obstructive pulmonary disease patients and healthy controls. Additionally, no significant association was retrieved between clinicopathologic parameters of chronic obstructive pulmonary disease, MMP-1 levels and this polymorphism. PMID: 27412345 In American cutaneous leishmaniasis caused by Leishmania braziliensis infection, MMP1 is regulated by factors other than FLI1, and the influence of IL-6 on MMP1 was independent of its effect on FLI1. PMID: 28119029 MMP1 was produced in the tumor compartment of prostate cancer bone metastases, highlighting its accessibility to KLK4 at this site PMID: 27378148 EREG and MMP-1 were found to be elevated in nasal polyp and uncinate tissues in patients with Chronic rhinosinusitis with nasal polyps. PMID: 28398769

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

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Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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