Recombinant Human MLCK Protein

Beta LifeScience SKU/CAT #: BL-0298SG
Recombinant Human MLCK Protein

Recombinant Human MLCK Protein

Beta LifeScience SKU/CAT #: BL-0298SG
Catalog No.: BL-0298SG

Product Overview

Tag GST
Host Species Human
Accession NM_053025
Synonym MYLK, KRP, MLCK108, MLCK210, MSTP083, FLJ12216, DKFZp686I10125
Background MLCK or myosin light chain kinase is a muscle member of the immunoglobulin gene superfamily and is a calcium/calmodulin dependent protein kinase. MLCK is a regulatory protein for smooth muscle contraction, which acts by phosphorylating 20-kDa myosin light chain (MLC20) to activate the myosin ATPase activity. The kinase activity for the phosphorylation is localized at the central part of MLCK, which is also furnished with actin-binding activity at its N terminal and myosin-binding activity at its C terminal (1). Phosphorylation of regulatory light chain of myosin plays an important role in controlling the morphological changes seen during cell division (2).
Description Recombinant human MLCK (1425-1776) was produced by baculovirus in Sf9 insect cells using a N-terminual GST tag. This protein is purified with our unique purification methods.
Source Sf9 insect cells
AA Sequence 1425-1776
Molecular Weight ~70 kDa
Purity For specific purity information on a given lot, see related COA.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Active
Formulation Recombinant protein is supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol.
Stability The recombinant protein is stable for up to 12 months at -70°C
Usage For Research Use Only
Storage Recombinant Human MLCK Protein should be stored should be stored at < -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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