Recombinant Human MLCK Protein
Beta LifeScience
SKU/CAT #: BL-0298SG
Recombinant Human MLCK Protein
Beta LifeScience
SKU/CAT #: BL-0298SG
Catalog No.: BL-0298SG
Product Overview
| Tag | GST |
| Host Species | Human |
| Accession | NM_053025 |
| Synonym | MYLK, KRP, MLCK108, MLCK210, MSTP083, FLJ12216, DKFZp686I10125 |
| Background | MLCK or myosin light chain kinase is a muscle member of the immunoglobulin gene superfamily and is a calcium/calmodulin dependent protein kinase. MLCK is a regulatory protein for smooth muscle contraction, which acts by phosphorylating 20-kDa myosin light chain (MLC20) to activate the myosin ATPase activity. The kinase activity for the phosphorylation is localized at the central part of MLCK, which is also furnished with actin-binding activity at its N terminal and myosin-binding activity at its C terminal (1). Phosphorylation of regulatory light chain of myosin plays an important role in controlling the morphological changes seen during cell division (2). |
| Description | Recombinant human MLCK (1425-1776) was produced by baculovirus in Sf9 insect cells using a N-terminual GST tag. This protein is purified with our unique purification methods. |
| Source | Sf9 insect cells |
| AA Sequence | 1425-1776 |
| Molecular Weight | ~70 kDa |
| Purity | For specific purity information on a given lot, see related COA. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Active |
| Formulation | Recombinant protein is supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol. |
| Stability | The recombinant protein is stable for up to 12 months at -70°C |
| Usage | For Research Use Only |
| Storage | Recombinant Human MLCK Protein should be stored should be stored at < -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
