Recombinant Human MICA Protein (C-6His)

Name: Recombinant Human MICA Protein (C-6His)
Brand: Beta LifeScience
SKU: BL-1227NP
Availability: InStock

BL-1227NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human MHC Class I Polypeptide-Related Sequence A is produced by our Mammalian expression system and the target gene encoding Glu24-Gln308 is expressed with a 6His tag at the C-terminus.
Accession	AAH16929.1
Synonym	MHC Class I Polypeptide-Related Sequence A; MIC-A; MICA; PERB11.1
Gene Background	MHC Class I Polypeptide-Related Sequence A (MICA) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. Unlike classical MHC class I molecules, MICA does not form a heterodimer with beta-2-microglobulin. MICA shares 85% amino acid identity with a closely related protein, MICB. MICA acts as a stress-induced self-antigen that is recognized by NK cells, NKT cells, and most of the subtypes of T cells. As a Ligand for the KLRK1/NKG2D receptor, MICA binds to KLRK1 leads to cell lysis. MICA functions as an antigen for gamma delta T cells and is frequently expressed in epithelial tumors. MICA antigens are able to elicit the synthesis of alloantibodies in transplant recipients. Studies have shown that anti-MICA antibodies are associated with acute renal allograft rejection and failure. MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.
Molecular Mass	33.87 KDa
Apmol Mass	60 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Biologically active. Please contact us to obtain bioactivity data.
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
Subcellular Location	Cell membrane; Single-pass type I membrane protein. Cytoplasm.
Protein Families	MHC class I family, MIC subfamily
Database References	HGNC: 7090 OMIM: 177900 KEGG: hsa:100507436 UniGene: PMID: 30181474 Role of MICA in different cancer types (review). PMID: 29635123 The MICA A5.1 polymorphism was associated with a better CT morphologic response to chemotherapy and a reduced risk of relapse after Colorectal Liver Metastases resection. PMID: 29969766 According to our analysis, three proteins, namely aristaless-like homeobox1 isoform X1 (ALX1), major histocompatibility complex polypeptide-related sequence A (MICA), and uncharacterized protein C14orf105 isoform X12 were found to be potential markers for Opisthorchis viverrini (OV)- infection, as they were predominantly found in all OV-infected groups PMID: 29936472 MICA-129 val/val genotype, associated with higher levels of circulating sMICA, may influence disease susceptibility and associate with increased severity of rheumatoid arthritis in south Indian Tamils. PMID: 28752674 rs12524487 in HLA-B/MICA was a genetic risk factor for Takayasu arteritis in a Chinese Han population and rs9366782 in this region was associated with ischemic brain disease in TA but not TA susceptibility. PMID: 28261975 Here we discovered that an HCMV protein named UL148A whose role was hitherto unknown is required for evasion of NK cells. We demonstrate that UL148A-deficient HCMV strains are impaired in their ability to downregulate MICA expression. PMID: 29950412 allele MICA002:01/A9 and haplotype MICA002:01-MICB005:02 were negatively associated with respiratory syncytial virus respiratory tract infections. PMID: 28925058 Levels of serum MICA were highly correlated to liver disease severity in chronic hepatitis C patients who carried the MICA rs738409 A allele. PMID: 28427234 Strategies to block MICA-NKG2D interactions resulted in reductions in IFNgamma production. Depletion of monocytes in vivo resulted in decreased IFNgamma production by murine NK cells upon exposure to Ab-coated tumor cells PMID: 28724544 A significant association was found between the Val allele and Val/Val genotype and the risk of breast cancer in Tunisian women. PMID: 28742417 the present study revealed that DNA damagedependent MICA/B expression in insensitive cancer cells can be restored by chromatin relaxation via the HDAC/Suv39/G9a pathway. Collectively, manipulation of chromatin status by therapeutic cancer drugs may potentiate the antitumor effect by enhancing immune activation following radiotherapy and DNA damage-associated chemotherapy. PMID: 28677817 Findings show that the efficient expression of cell-surface major histocompatibility class I-related chain molecule A (MICA) in the osteosarcoma cells requires asparagine (Asn)-N-linked glycosylation of MICA. PMID: 29491059 Study identified the novel role of transcription factors GATA-2 and GATA-3 in suppressing MICA/B expression in HBV-infected human hepatoma cells. PMID: 27528231 new MICA allelic variant, MICA007:07, was identified in an individual of Mongol ethnicity in the Inner Mongolia Autonomous Region, northern China PMID: 28371368 inhibition of BET proteins can enhance the expression of MICA, a ligand of the NKG2D receptor, in human MM cell lines and primary malignant plasma cells, rendering myeloma cells more efficient to activate NK cell degranulation PMID: 27903272 results indicate that the polymorphism of rs2256318 in MICA may contribute to the etiology of Preterm birth through interfering with placental development. These findings need to be further validated in larger and multi-ethnic populations. PMID: 28864994 The results of this study suggested that the MICB009N allele might be a risk factor for SLE, whereas the MICB014, MICA010 and MICB002 alleles were associated with reduced incidence of SLE in the study population. PMID: 29078849 New truncated MICA isoforms exhibit a range of functions that may drive unexpected immune mechanisms and provide new tools for immunotherapy PMID: 27342847 data reveal that MICA and PVR are directly regulated by human cytomegalovirus immediate early proteins, and this may be crucial for the onset of an early host antiviral response PMID: 27733551 This demonstrates for the first time that MICA/B is more broadly expressed in normal tissue and that expression is mainly intracellular with only a small fraction appearing on the cell surface of some epithelia and tumour cells. PMID: 28334733 selecting a MICA-matched donor significantly influences key clinical outcomes of HCT in which a marked reduction of GVHD is paramount. The tight linkage disequilibrium between MICA and HLA-B renders identifying a MICA-matched donor readily feasible in clinical practice. PMID: 27549307 MICA-129 matching is relevant in unrelated hematopoietic stem cell transplantation. PMID: 27811019 No evidence of an association between MICADel and nasopharyngeal carcinoma in the southern Chinese Han population. PMID: 27870115 These findings identify the major histocompatibility complex-related MICA as an immunogenetic factor that may functionally influence anti-BK polyomavirus immune responses and infection outcomes. PMID: 27130430 This study provided information on the distribution of MICA polymorphisms and linkage disequilibrium with HLA-B alleles in Brazilian renal-transplant candidates. A total of 19 MICA allele groups were identified. The most frequent allele groups were MICA008 (21.6%), MICA002 (17.0%) and MICA004 (14.8%). The most common haplotypes were MICA009-B51 (7.8%), MICA004-B44 (6.06%) and MICA002-B35 (5.63%). PMID: 28419176 MICAA4 protects against ulcerative colitis, whereas MICAA5.1 is associated with abscess formation and age of onset. PMID: 26940143 MICA012:05 differs from MICA012:01 by a single synonymous C to T substitution at nucleotide position 269 in exon PMID: 27273902 there is no obvious correlation between the MMP9 -1562 C/T SNP and the concentrations of circulating MICA/B in the breast cancer patients. PMID: 27026046 Cirrhotic patients who carry MICA risk alleles and those without risk alleles but with high sMICA levels possessed the highest risk of HCC development once they failed antiviral therapy. PMID: 27998720 High levels of serum MICA is associated with acute myocardial infarction. PMID: 27306684 Findings shown here are important from an anthropologic perspective and will inform future studies of the potential role of MICA and MICB genes in allogeneic organ transplantation and HLA-linked disease association in populations of related ancestry. PMID: 27028549 MICA was significantly associated with the epithelial-to-mesenchymal transition gene set in clear cell renal cell carcinoma PMID: 26349747 Reversal of epigenetic silencing of MICA and MICB improves immune recognition and killing of Merkel cell carcinoma cells. PMID: 26902929 Our meta-analysis confirmed MICA-A6 could be responsible for BD in three ethnic regions and should probably be treated as a risk factor for Behcet's disease. PMID: 26875668 IFN-gamma rs2069727 and MICA rs2596542 polymorphisms may be related to the incidence of hepatocellular carcinoma. PMID: 26893439 gastric cancer patients who have tumors with high expression of MICA are more likely to benefit from adjuvant chemotherapy, immunotherapy and gastrectomy PMID: 26607264 Our results suggest that locally sustained expression of MICA and MICB in the tumor may contribute to the malignant progression of Gastric cancer(GC) and that expression of these ligands predicts an unfavorable prognosis in GC patients presenting large tumors. PMID: 26708143 In South Tunisian population, MICA plays a disease modifying role, rather than being an important gene in the susceptibility for developing of uveitis. PMID: 25468490 The reduced cell surface expression of NKG2D in response to engagement by MICA-129Met variants appeared to reduce the severity of acute graft-versus-host disease. PMID: 26483398 Estrogen upregulates MICA/B expression in human non-small cell lung cancer through the regulation of ADAM17. PMID: 25363527 This meta-analysis shows that the MICA-TM A6 allele and the MICA009 allele are associated with BD susceptibility in various ethnic populations, and that MICA alleles are in strong linkage disequilibrium with HLA-B51 in BD. PMID: 26184953 The importance of the functional MICA-129 polymorphism in the severity of left ventricular ejection fraction in Chronic Chagas heart disease was addressed. PMID: 26129751 MICA polymorphisms do not appear to influence the development of ocular lesions in patients diagnosed with toxoplasmosis in this study population. PMID: 26672749 The study investigates the link between Behcet's Disease and two specific HLA alleles associated with Behcet's Disease (HLA-A26:01 and HLA-B*51:01) in terms of their binding affinity to the MICA. PMID: 26331842 In conclusion DNT cell can significantly inhibit the growth of pancreatic carcinoma in vivo, and the mechanism may be involved in abnormal expressions of MICA and NKG2D. PMID: 26616050 Further studies of the association between HBV replication and MICA induction should be conducted. PMID: 26212443 In cervical adenocarcinoma (but not squamous cell carcinoma), low sMICA was positively related to recurrent disease, a higher FIGO stage and vaginal involvement. High sMICA levels were associated with better disease-free and disease-specific survival. PMID: 25871737 Serum MICA levels were related to tumor pathology, TNM stage, and kidney neoplasm metastasis. PMID: 26125933 High MICA expression is associated with Renal Cancer. PMID: 25987057

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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