Recombinant Human Mhc Class I Polypeptide-Related Sequence B Protein (MICB) Protein (His/Tag-Free)

Beta LifeScience SKU/CAT #: BLC-05300P

Recombinant Human Mhc Class I Polypeptide-Related Sequence B Protein (MICB) Protein (His/Tag-Free)

Beta LifeScience SKU/CAT #: BLC-05300P
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Product Overview

Description The Recombinant Human Mhc Class I Polypeptide-Related Sequence B Protein is produced by our E.coli expression system.
Purity Greater than 85% as determined by SDS-PAGE and HPLC.
Uniprotkb Q29980
Target Symbol MICB
Synonyms MHC class I chain related protein B; MHC class I chain related protein B2; MHC class I like molecule PERB11.2 IMX; MHC class I mic B antigen; MHC class I polypeptide related sequence B; MHC class I polypeptide-related sequence B; MIC B; MIC-B; MICB; MICB_HUMAN; PERB11.2; Stress inducible class I homolog
Species Homo sapiens (Human)
Expression System E.coli
Tag N-His/Tag-Free
Complete Sequence AEPHSLRYNL MVLSQDESVQ SGFLAEGHLD GQPFLRYDRQ KRRAKPQGQW AEDVLGAKTW DTETEDLTEN GQDLRRTLTH IKDQKGGLHS LQEIRVCEIH EDSSTRGSRH FYYDGELFLS QNLETQESTV PQSSRAQTLA MNVTNFWKED AMKTKTHYRA MQADCLQKLQ RYLKSGVAIR RTVPPMVNVT CSEVSEGNIT VTCRASSFYP RNITLTWRQD GVSLSHNTQQ WGDVLPDGNG TYQTWVATRI RQGEEQRFTC YMEHSGNHGT HPVPSGKVLV LQSQRTD
Mol. Weight 32.8 kDa
Form Liquid or Lyophilized powder
Buffer Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.

Target Details

Target Function Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
Subcellular Location Cell membrane; Single-pass type I membrane protein.
Protein Families MHC class I family, MIC subfamily
Database References

HGNC: 7091

OMIM: 180300

KEGG: hsa:4277

STRING: 9606.ENSP00000252229

UniGene: PMID: 28925058

  • the present study revealed that DNA damagedependent MICA/B expression in insensitive cancer cells can be restored by chromatin relaxation via the HDAC/Suv39/G9a pathway. Collectively, manipulation of chromatin status by therapeutic cancer drugs may potentiate the antitumor effect by enhancing immune activation following radiotherapy and DNA damage-associated chemotherapy. PMID: 28677817
  • The results of this study suggested that the MICB*009N allele might be a risk factor for SLE, whereas the MICB*014, MICA*010 and MICB*002 alleles were associated with reduced incidence of SLE in the study population. PMID: 29078849
  • No association between MICB genotype and early viremia level in dengue patients. PMID: 28599625
  • This demonstrates for the first time that MICA/B is more broadly expressed in normal tissue and that expression is mainly intracellular with only a small fraction appearing on the cell surface of some epithelia and tumour cells. PMID: 28334733
  • this study shows that vigilin (HDLBP) binds and negatively regulates MICB expression through its 5'UTR, and that vigilin downregulation in target cells leads to NK cell activation against said target cells PMID: 28356383
  • we have found that the allele G of rs3828903 was significantly associated with risk susceptibility to SLE in the current population. These data together with the functional annotations of rs3828903 convert MICB into a main candidate for being an additional MHC gene associated with SLE susceptibility. PMID: 27433477
  • this study presents the impact of a single nucleotide transition at SNPs associated with DSS on differential protein binding patterns with PLCE1 and MICB genes and on protein structure modification and their possible role in the pathogenesis of dengue shock syndrome PMID: 27038471
  • there is no obvious correlation between the MMP9 -1562 C/T SNP and the concentrations of circulating MICA/B in the breast cancer patients. PMID: 27026046
  • Findings shown here are important from an anthropologic perspective and will inform future studies of the potential role of MICA and MICB genes in allogeneic organ transplantation and HLA-linked disease association in populations of related ancestry. PMID: 27028549
  • IMP3 directly interacts with ULBP2 mRNA, leading to ULBP2 transcript destabilization and reduced ULBP2 surface expression and indirectly downregulates MICB with a mechanism functionally distinct from that of ULBP2. PMID: 26982091
  • Reversal of epigenetic silencing of MICA and MICB improves immune recognition and killing of Merkel cell carcinoma cells. PMID: 26902929
  • We have now found a total of 15 individual MICB promoter sequences, varying by combination of 18 polymorphic positions within the MICB minimal promoter sequence. PMID: 26707708
  • Our results suggest that locally sustained expression of MICA and MICB in the tumor may contribute to the malignant progression of Gastric cancer(GC) and that expression of these ligands predicts an unfavorable prognosis in GC patients presenting large tumors. PMID: 26708143
  • Estrogen upregulates MICA/B expression in human non-small cell lung cancer through the regulation of ADAM17. PMID: 25363527
  • Aligned with MICB*005:02, MICB*030 has a nonsynonymous adenine substitution at nucleotide position 50 in exon 3, leading to amino acid change from serine to arginine at codon 102 of the mature MICB molecule. PMID: 25990310
  • We have demonstrated a novel mechanism by which tumor-derived sMIC may temper the immune reactive tumor microenvironment. PMID: 25887583
  • This study suggests that NKG2D ligands shedding of MICA, MICB and ULBP-2 is a novel pathway in endometriosis complex pathogenesis that impairs Natural Killer Cells cell function. PMID: 25775242
  • six RNA-binding proteins that bind and regulate the expression of MICB. PMID: 24924487
  • The sMICB serum levels were significantly increased in hepatitis B patients compared to healthy controls. The sMICB serum levels were decreased in a treated hepatocellular carcinoma (HCC) patient group compared to a not-treated HCC patient group. PMID: 25626490
  • MICA/B expression is inhibited by unfolded protein response and associated with poor prognosis in human hepatocellular carcinoma. PMID: 25228093
  • Genotoxic stress induces senescence-associated ADAM10-dependent release of NKG2D MIC ligands (MICA, MICB) in multiple myeloma cells. PMID: 26071561
  • Data indicate that MHC class I chain-related protein A (MICA)( *)010, MICA( *)A5 and MHC class I chain-related protein B (MICB)( *)005:02 were the most frequent alleles. PMID: 25167773
  • The elevation of soluble MICB during acute primary Dengue virus infections in infants likely represents an immune evasion strategy and contributes to the severity of the acute illness. PMID: 24869966
  • If differential expression by polymorphic MICA and MICB promoters is confirmed by functional studies, involvement of these genes in disease susceptibility or adverse transplantation outcomes may require knowledge of both promoter and allelic types to make meaningful conclusions. PMID: 24962621
  • MICb expression in macrophage foam cells infiltrating atherosclerotic plaques PMID: 24997223
  • Experiments demonstrate that infection in vitro with human cytomegalovirus leads to enhanced shedding of MICA and MICB. PMID: 24973455
  • Poorly differentiated tumors showed high MICA/B expression, which was related to extended tumor lymph node metastases and less frequent long-term survival. PMID: 24173243
  • we conclude that rs3132468-C at MICB and rs3765524-C at PLCE1 confer risk of DSS in Southeast Asians. PMID: 24884822
  • Broad MICA and MICB expression in the small bowel mucosa suggest a link between cellular stress and celiac disease. PMID: 24058482
  • Hepatitis B surface antigen inhibits MICA and MICB expression via induction of cellular miRNAs in hepatocellular carcinoma cells. PMID: 23917076
  • Diverse MICB alleles differentially bound cytomegalovirus UL16. PMID: 23625227
  • the MICB rs3132468 and PLCE1 rs3740360 genotypes are associated with clinically apparent dengue in both adults and children. PMID: 23536857
  • Soluble and membrane-restricted NKG2D ligand, MICB, poses opposite impacts on prostate cancer progression. PMID: 24018560
  • study observed a substantial heterogeneity among the different cancer cell lines with regard to the involvement of ADAM10 and/or ADAM17 in MICA or MICB shedding PMID: 23526433
  • 17beta-estradiol upon forming a complex with its cognate receptor suppresses MICB expression through binding with SP1/SP3 sites within the MICB promoter GC box. PMID: 23527639
  • The frequencies of 20 MICB alleles and their haplotypes among 400 unrelated Han persons was determined. 5 new alleles, MICB*005:07, MICB*005:08, MICB*027, MICB*028, & MICB*029 were found.MICB*028 probably arose from MICB*004:01:01. PMID: 23549730
  • MICA and MICB were upregulated in esophageal cancer. The expression of MICA/B was related with histological grade. PMID: 23291067
  • Two new MICB alleles, MICB*005:06 and MICB*026, have been identified in a southern Chinese Han population. PMID: 22609444
  • our results define MICB as a novel immune target of miR-10b PMID: 22915757
  • Expression of FimX produces bidirectional inversion of the hyxR promoter region and promotes HyxR expression. HyxR suppresses intracellular survival in macrophages through reactive nitrogen intermediate-dependent and -independent mechanisms. PMID: 22221182
  • MICA and MICB molecules act as key ligands for activating receptor natural killer cell (NK) group 2, member D (NKG2D) and promote NK cell-mediated recognition and cytolysis. PMID: 22301547
  • high expression of MICB gene in Multiple sclerosis (MS) patients is an important criterion of MS disease that it may be due to the interaction between MICB and its receptor on CD8+T or NK cells. PMID: 22184268
  • data suggest that posttranslational N-linked glycosylation is strictly required for NKG2D ligand, MICB, surface expression PMID: 22227571
  • Data show that VSV infection caused an active suppression of NKG2D-ligand surface expression, affecting both endogenous and histone deacetylase (HDAC)-inhibitor induced MICA, MICB and ULBP-2 expression. PMID: 21857986
  • The positive expression rate of MHC-I A/B and HLA-E in ALL patients was obviously higher than that in AML patients. PMID: 21518478
  • Statistical analysis revealed a tendency for MICA*008 and MICB*008 to associate with susceptibility to illness when symptomatic versus asymptomatic cases of Dengue were compared. PMID: 21762746
  • identified a susceptibility locus at MICB (major histocompatibility complex (MHC) class I polypeptide-related sequence B), which was within the broad MHC region on chromosome 6 but outside the class I and class II HLA loci, per-allele odds ratio PMID: 22001756
  • Information about MICB allele frequencies and haplotypes with HLA in Koreans. PMID: 21554252
  • The levels of MICB expression in serum and tissue of pancreatic cancer are elevated PMID: 21605422

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    Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

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    Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

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