Recombinant Human Metabotropic Glutamate Receptor 5 (GRM5) Protein (His&Myc)

Name: Recombinant Human Metabotropic Glutamate Receptor 5 (GRM5) Protein (His&Myc)
Brand: Beta LifeScience
SKU: BLC-04935P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Metabotropic Glutamate Receptor 5 (GRM5) Protein (His&Myc) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	P41594
Target Symbol	GRM5
Synonyms	Glutamate receptor metabotropic 5; GPRC1E; Grm5; GRM5_HUMAN; Metabotropic glutamate receptor 5; Metabotropic glutamate receptor 5 variant F; Metabotropic glutamate receptor 5 variant G; Metabotropic glutamate receptor 5 variant H; mGlu5; mGluR5; PPP1R86; Protein phosphatase 1 regulatory subunit 86
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-10His&C-Myc
Target Protein Sequence	SSERRVVAHMPGDIIIGALFSVHHQPTVDKVHERKCGAVREQYGIQRVEAMLHTLERINSDPTLLPNITLGCEIRDSCWHSAVALEQSIEFIRDSLISSEEEEGLVRCVDGSSSSFRSKKPIVGVIGPGSSSVAIQVQNLLQLFNIPQIAYSATSMDLSDKTLFKYFMRVVPSDAQQARAMVDIVKRYNWTYVSAVHTEGNYGESGMEAFKDMSAKEGICIAHSYKIYSNAGEQSFDKLLKKLTSHLPKARVVACFCEGMTVRGLLMAMRRLGLAGEFLLLGSDGWADRYDVTDGYQREAVGGITIKLQSPDVKWFDDYYLKLRPETNHRNPWFQEFWQHRFQCRLEGFPQENSKYNKTCNSSLTLKTHHVQDSKMGFVINAIYSMAYGLHNMQMSLCPGYAGLCDAMKPIDGRKLLESLMKTNFTGVSGDTILFDENGDSPGRYEIMNFKEMGKDYFDYINVGSWDNGELKMDDDEVWSKKSNIIRSVCSEPCEKGQIKVIRKGEVSCCWTCTPCKENEYVFDEYTCKACQLGSWPTDDLTGCDLIPVQYLRWGDPEP
Expression Range	22-580aa
Protein Length	Partial
Mol. Weight	70.6 kDa
Research Area	Cancer
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.
Subcellular Location	Cell membrane; Multi-pass membrane protein.
Protein Families	G-protein coupled receptor 3 family
Database References	HGNC: 4597 OMIM: 604102 KEGG: hsa:2915 STRING: 9606.ENSP00000306138 UniGene: PMID: 29317611 We anticipate that the resulted outcome could be supportive to discover potent negative allosteric modulators for metabotropic glutamate receptor 5 (mGluR5). PMID: 29806525 This study provides information concerning the changes in the expression of GABAergic and glutamatergic markers in mice lacking the mGlu5. PMID: 27581816 We report that expression of a key regulator of synaptic protein synthesis, the metabotropic glutamate receptor 5 (mGlu5) protein, is significantly reduced in both the brains of RS model mice and in the motor cortex of human RS autopsy samples. PMID: 26936821 once at the inner nuclear membrane, mGluR5 is stably retained via interactions with chromatin and is perfectly positioned to regulate nucleoplasmic Ca(2+)in situ PMID: 28096465 Overall, these data posit mGluR5 in key paralimbic areas as a strong determinant of the temperament trait novelty seeking. These data add to our understanding of how brain neurochemistry accounts for the variation in human behavior and strongly support further research on mGluR5 as a potential therapeutic target in neuropsychiatric disorders associated with abnormal novelty-seeking behaviors. PMID: 27283933 Decrease of mGluR5 receptor density goes parallel with changes in enkephalin and substance P immunoreactivity in Huntington's disease PMID: 24969128 The metabotropic glutamate receptor subtype 5 (mGluR5) has been implicated in the pathophysiology of mood and anxiety disorders. PMID: 26645628 The interaction between GRM5 and cellular prion protein has a central role in transgenic mouse model of Alzheimer's disease pathogenesis. PMID: 26667279 Data suggest that metabotropic glutamate receptors recycling is completely dependent on the activity of PP2A whereas, PP2B has partial effect on this process. PMID: 26311002 findings provide evidence for decreased expression of metabotropic glutamate receptor 5 and its signaling components representing a key pathophysiological hallmark in autism spectrum disorder. PMID: 26052099 Study provided evidence that protein expression of mGluR5 is significantly higher (total: 42%; monomer: 25%; dimer: 52%) in the hippocampal CA1 region of schizophrenia subjects relative to healthy controls PMID: 26048293 Results provide compelling evidence that mGluR5 regulation is altered in schizophrenia, likely contributing to the altered glutamatergic signaling that is associated with the disorder PMID: 25778620 This review supports altered mGluR5 functioning as a convergent point in ASD pathogenesis and indicates more research is warranted into mGluR5 as a potential therapeutic target. PMID: 25704074 both an increased and reduced mGlu5 functioning seem to be associated with Intellectual Disability and autism spectrum disorders. PMID: 24548786 significant positive correlation between Yale-Brown Obsessive Compulsive Scale obsession sub-score and mGluR5 distribution volume ratio in the cortico-striatal-thalamo-cortical brain circuit; results suggest obsessions in particular might have an underlying glutamatergic pathology related to mGluR5 PMID: 24833114 gnificantly higher expression level in the metabotropic receptor genes GRM5 in Locus coeruleus neurons. PMID: 24925192 Findings demonstrate a developmental regulation of mGluR5 in the hippocampus and suggest a role for this receptor in astrocytes during early development in Down's syndrome hippocampus PMID: 25115540 findings suggest that changes in astrocyte mGlu5 receptors may be part of an adaptive response to the progressive nature of Alzheimer's disease PMID: 19401173 tobacco smoking was associated with lower mGluR5 availability in the brain in both cocaine users and controls, while cocaine use was not linked to detectable mGluR5 alterations PMID: 23628984 Our findings highlight the subtleties of allosteric modulator binding to mGlu5 and demonstrate the utility in incorporating SAR information to strengthen the interpretation and analyses of docking and mutational data. PMID: 24528109 a dysregulation of its signaling in fragile X mental retardation gene 1 premutation carriers, which would likely contribute to the development and severity of fragile X-associated tremor/atasia syndrome. PMID: 24332449 reflected in the immunoblotting data Mglur5 receptors were detected at higher levels of expression in patient with cortical dysplasia with intractable epilepsy. PMID: 25003238 Review provides an update of the current state of the art of mGlu5 receptor-based manipulations to alleviate the symptoms of Parkinson's disease. PMID: 24040811 The pattern of mGluR5 expression by neural stem/progenitor cells, neuroblasts and neurons provides important anatomical evidence for the role of mGluR5 in the regulation of human hippocampal development PMID: 23225313 This study demonistrated that mGluR5 upregulation also participates in counterbalance mechanisms along the hyperexcitable circuitry uniquely altered in TLE hippocampal formation. PMID: 23804486 This study demonistrated that metabotropic receptor mGluR5 was found to be expressed by just 40% of striatal neurons in young individuals, with significant intensity variations among the neurons. PMID: 23627706 this study provides evidence that GRK2 mediates phosphorylation-independent mGluR5 desensitization via the interaction between the RGS domain and Galphaq in HEK 293 cells. PMID: 23705503 Knockdown of mGluR5 inhibited icariin induced reactive oxygen species generation and NF-kappaB nuclear translocation. PMID: 23524143 mGluR5 inhibition and stimulation in the nucleus accumbens can regulate cocaine-seeking, and demonstrate that one mechanism for this effect is via interactions with Homer proteins PMID: 22340009 Plasma glutamate-modulated interaction of A2AR and mGluR5 on BMDCs aggravates traumatic brain injury-induced acute lung injury. PMID: 23478188 mGluR5 is expressed by a variety of cells such as neural stem cells in the frontal cortex, ventricular zone and subventricular zone in human fetuses. PMID: 22543119 data demonstrate that mGluR5 receptors internalize without the application of ligand and the internalized receptors recycle back to the cell surface following constitutive endocytosis. PMID: 22995293 Calmodulin-regulated Siah-1A binding to mGluR5 dynamically regulates mGluR5 trafficking PMID: 23152621 Human mGluR5, GABA(A,B), and CB(1,2) receptors are abundantly expressed along the vago-vagal neural pathway and involved in the triggering of transient lower esophageal sphincter relaxations PMID: 22256945 the results of this study show that using the equilibrium method is an acceptable alternative to the standard kinetic method when using 18F-SP203 to measure mGluR5 PMID: 22032949 Elevated glutamatergic transmission as measured with increased mGluR5 specific binding is associated with human motor complications; its antagonism can be targeted for patients' treatment. PMID: 20036444 Metabotropic glutamate receptor 5 upregulation in children with autism is associated with underexpression of both Fragile X mental retardation protein and GABAA receptor beta 3 in adults with autism. PMID: 21901840 mGluR5 promoted the proliferation of human embryonic cortical neural stem/progenitor cells PMID: 21723923 Results highlight the therapeutic importance of mGluR5 antagonists in alpha-synucleinopathies. PMID: 21103359 mGLUR5 and 1,4,5-InsP3 signaling control calcium release PMID: 12119301 Data report the isolation of a novel gene termed metabotropic glutamate receptor 5-related (mGluR5R) [mGluR5R]. PMID: 12531512 Data report the identification of a novel variant of the G-protein coupled metabotropic glutamate receptor mGlu5 (hmGlu5d) generated by alternative splicing at the C-terminal domain. PMID: 12531526 mGlu5 on nuclear membranes has a role in mediating intranuclear Ca2+ changes in heterologous cell types and neurons PMID: 12736269 alternative 5'-splicing and usage of multiple promoters may contribute to regulatory mechanisms for tissue- and context-specific expression of the mGluR5 gene PMID: 12783878 activation of mGluR5 causes translocation of both gammaPKC and deltaPKC to the plasma membrane. deltaPKC, but not gammaPKC, phosphorylates mGluR5 Thr(840), leading to the blockade of both Ca2+ oscillations and gammaPKC cycling. PMID: 14561742 We found a selective expression of the group I receptor mGluR5 in human parasympathetic Onuf's nucleus, strengthening the hypothesis that mGluR expression may provide a possible clue to the selective vulnerability in ALS. PMID: 15076751 it is phosphorylation of Ser-839, not Thr-840, that is absolutely required for the unique Ca2+ oscillations produced by mGluR5 activation PMID: 15894802 there is an alpha-actinin-1-dependent mGlu(5b) receptor association with the actin cytoskeleton modulating receptor cell surface expression and functioning PMID: 17311919 We documented for the first time the expression of the mGluR5 and EAAT1 in MG-63 cells, as well as the ability of dexamethasone to upregulate the expression of the mGluR5 and EAAT1 in the MG-63 cells. PMID: 17627080

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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