Recombinant Human MDH1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BL-0299NP
Recombinant Human MDH1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BL-0299NP
Catalog No.: BL-0299NP
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | P40925 |
| Synonym | MDH1 Malate Dehydrogenase Cytoplasmic; Cytosolic Malate Dehydrogenase; Diiodophenylpyruvate Reductase; MDH1; MDHA |
| Background | Malate Dehydrogenase, Cytoplasmic (MDH1) is an enzyme which belongs to the MDH Type 2 sub-family of LDH/MDH superfamily. MDH1 is involved in the Citric Acid Cycle that catalyzes the conversion of Malate into Oxaloacetate (using NAD+) and vice versa. MDH1 should not be confused with Malic Enzyme, which catalyzes the conversion of Malate to Pyruvate, producing NADPH. MDH1 also participates in Gluconeogenesis, the synthesis of Glucose from smaller molecules. Pyruvate in the mitochondria is acted upon by Pyruvate Carboxylase to form Pxaloacetate, a Citric Acid Cycle intermediate. In order to transport the Oxaloacetate out of the Mitochondria, Malate Dehydrogenase reduces it to Malate, and it then traverses the inner Mitochondrial membrane. Once in the cytosol, the Malate is oxidized back to Oxaloacetate by MDH1. Finally, Phosphoenol-Pyruvate Carboxy Kinase (PEPCK) converts Oxaloacetate to Phosphoenol Pyruvate. |
| Description | Recombinant Human Malate Dehydrogenase, Cytoplasmic is expressed by our E.coli expression system and the target gene encoding Ser2-Ala334 is expressed with a His tag at the C-terminus. |
| Source | E.coli |
| AA Sequence | Cytoplasmic |
| Purity | >95% as determined by reducing SDS-PAGE. |
| Endotoxin | <0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| Bioactivity | Measured by its dehydrogenation activity from (S)-malate to oxaloacetate in the presense of NAD+Specific Activity is greater than 1000 pmol/min/ug |
| Formulation | Recombinant Human MDH1 Protein was supplied as a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH 8.0. |
| Usage | For Research Use Only |
| Storage | Human MDH1 protein should be stored at < -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles. |
