Recombinant Human Lutropin-Choriogonadotropic Hormone Receptor (LHCGR) Protein (His)

Beta LifeScience SKU/CAT #: BLC-09563P
Greater than 90% as determined by SDS-PAGE.
Greater than 90% as determined by SDS-PAGE.

Recombinant Human Lutropin-Choriogonadotropic Hormone Receptor (LHCGR) Protein (His)

Beta LifeScience SKU/CAT #: BLC-09563P
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

Product Overview

Description Recombinant Human Lutropin-Choriogonadotropic Hormone Receptor (LHCGR) Protein (His) is produced by our Yeast expression system. This is a protein fragment.
Purity Greater than 90% as determined by SDS-PAGE.
Uniprotkb P22888
Target Symbol LHCGR
Synonyms Gonadotropin receptor; Gpcr19-rs1; GTHR-II; HHG; LCGR; LGR2; LH-R; LH/CG R; LH/CG-R; LH/CGR; LHCGR; LHR; LHRHR; LSH R; LSH-R; LSHR_HUMAN; Luteinizing hormone receptor; Luteinizing hormone/choriogonadotropin receptor; Lutropin choriogonadotropic hormone receptor ; Lutropin choriogonadotropic receptor ; Lutropin-choriogonadotropic hormone receptor; ULG5
Species Homo sapiens (Human)
Expression System Yeast
Tag N-6His
Target Protein Sequence EALCPEPCNCVPDGALRCPGPTAGLTRLSLAYLPVKVIPSQAFRGLNEVIKIEISQIDSLERIEANAFDNLLNLSEILIQNTKNLRYIEPGAFINLPRLKYLSICNTGIRKFPDVTKVFSSESNFILEICDNLHITTIPGNAFQGMNNESVTLKLYGNGFEEVQSHAFNGTTLTSLELKENVHLEKMHNGAFRGATGPKTLDISSTKLQALPSYGLESIQRLIATSSYSLKKLPSRETFVNLLEATLTYPSHCCAFRNLPTKEQNFSHSISENFSKQCESTVRKVNNKTLYSSMLAESELSGWDYEYGFCLPKTPRCAPEPDAFNPCEDIMGYDFLR
Expression Range 27-363aa
Protein Length Partial
Mol. Weight 39.6kDa
Research Area Neuroscience
Form Liquid or Lyophilized powder
Buffer Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function Receptor for lutropin-choriogonadotropic hormone. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.
Subcellular Location Cell membrane; Multi-pass membrane protein.
Protein Families G-protein coupled receptor 1 family, FSH/LSH/TSH subfamily
Database References
Associated Diseases Familial male precocious puberty (FMPP); Luteinizing hormone resistance (LHR)
Tissue Specificity Gonadal and thyroid cells.

Gene Functions References

  1. This study demonstrates that both gonadotrophin receptors9 FSH receptor (FSHR) and LH/choriogonadotrophin receptor (LHCGR) are expressed along the glandular epithelium of endometria and they mediate the effects of gonadotrophins on intracellular functions. PMID: 29974367
  2. Genotyping analysis showed no association between luteinizing hormone receptor (LHCGR) rs68073206 and male infertility, however, this polymorphism was significantly associated with testosterone levels in normozoospermic subjects. PMID: 28367994
  3. Data suggest that, during follicle maturation, levels of MIR122 determine levels of LHR mRNA expression in the ovary. (LHR = luteinizing hormone receptor; MIR122 = microRNA 122) [REVIEW] PMID: 29544643
  4. genetic association/pharmacogenetic studies in population in India: Data suggest that need for (and dose required of) fertility agent recombinant LH in addition to fertility agent recombinant FSH is associated with SNP in LHCGR in women undergoing ovarian stimulation in conjunction with in vitro fertilization. (LH = luteinizing hormone; FSH = follicle stimulating hormone) PMID: 29408742
  5. hCG and its receptor, LH/CGR, are expressed in numerous sites of the reproductive tract, both in gonadal and extra-goanadal tissues, promoting oocyte maturation, fertilization, implantation and early embryo development PMID: 28505106
  6. The reduced fertilisation and pregnancy rate was associated with a lower LH receptor density and a lack of essential down-regulation of the FSH and LH receptor. PMID: 28188844
  7. This work demonstrates that the expression of FSHR and LHCGR can be induced in hGL5 cells but that the FSHR-dependent cAMP/PKA pathway is constitutively silenced, possibly to protect cells from FSHR-cAMP-PKA-induced apoptosis. PMID: 27502035
  8. Inactivating mutations of the LHCGR gene may be a common cause of 46,XY primary amenorrhea. PMID: 27016457
  9. data demonstrate that the majority of LHR mutations lead to intracellular retention and highlight the potential for novel pharmacological chaperone therapeutics that can "rescue" expression/function of retained mutant GPCRs. PMID: 27533885
  10. expression and activation of LHCGR and ARF6 are up-regulated in GC from PCOS women but the mechanism of agonist-induced LHCGR internalization is unaltered PMID: 27061682
  11. We showed that GNRHR and LHCGR were highly expressed in some wildtype aldosterone-producing adenoma samples, and that they positively correlated with GnRH-stimulated aldosterone production. PMID: 27196470
  12. Mutation in the LHCGR gene is associated with Testotoxicosis. PMID: 26757946
  13. The luteinizing hormone/human chorionic gonadotrophin receptor (LHCGR) variant N312S and the follicle-stimulating hormone receptor (FSHR) variant N680S can be utilized for the prediction of pregnancy chances in women undergoing IVF. PMID: 26769719
  14. Data indicate two patients with peripheral precocious puberty and an activating mutation in the luteinizing hormone (LH))/choriogonadotropin receptor (LHCGR) gene. PMID: 26831561
  15. The expression of miR-592 and its effects on LHCG receptors in patients with polycystic ovary syndrome and normal controls are described. PMID: 26679164
  16. LHCGR levels in circulation predicts early and late preeclampsia in first trimester of pregnancy. PMID: 25676660
  17. Data suggest that rs13405728 single nucleotide polymorphism of the luteinizing hormone receptor (LHR) gene may be associated with slow ovarian response in assisted reproductive technology (ART). PMID: 26663062
  18. The insLQ polymorphism within LHR is associated with endometriosis and infertility. PMID: 25935136
  19. the LHR can act in an invertebrate demonstrates the conservation of factors required for GPCR function among phylogenetically distant organisms. PMID: 26112185
  20. Demonstrate the presence of LH receptors. Activation resulted in a dose-dependent increase in glucose-induced release of insulin. PMID: 25670721
  21. Our aim was to study the association of LHCGR polymorphism (rs2293275) with PCOS PMID: 25565299
  22. Melatonin affects the secretion of GnRH, LH and testosterone, improves sperm quality thereby regulating the testicular development and male reproduction. (Review) PMID: 25916694
  23. This is the first study to confirm the association of novel LHCGR and FSHR SNPs with PCOS. The differential association of LHCGR and FSHR variants with PCOS confirms the racial/ethnic contribution to their association with PCOS. PMID: 25649397
  24. Case Report: LHCGR nonsense mutation in an adult 46,XY female patient causing severe Leydig cell hypoplasia. PMID: 25079470
  25. A high prevalence of endometriosis, polymorphism in the LHCGR and GnRH1 and progonadoliberin-2 antibodies in serum was found among the patients with severe dysmotility after treatment with GnRH analogs. PMID: 25592315
  26. affected patients were all homozygous for a novel mutation in the LHCGR gene, a deletion of guanine in position 1850 (1850delG);the novel LHCGR mutation leads to complete inactivation of the LHCGR receptor due to trafficking and signaling abnormalities PMID: 25795638
  27. Data indicate that all solid pseudopapillary tumor of the pancreas {SPT) presented membranous and cytoplasmic staining for luteinizing hormone receptor (LH receptor). PMID: 25010708
  28. Data suggest LHR expression is up-regulated in granulosa cells and theca cells of early antral follicles, as follicles grow/mature, and in corpus luteum; low but significant expression of LHR is exhibited in cumulus cells (but not in stromal cells). PMID: 24874553
  29. this is the first report suggesting that LHCGR genetic variation might function in patient risk for ovarian hyperstimulation syndrome PMID: 23883350
  30. Silencing luteinizing hormone receptor expression suppresses androgen synthesis in prostate cancer cells. PMID: 25393079
  31. genetic association study (case-control study) in population in Egypt: Data suggest that an SNP in LHCGR (rs2293275) is associated with polycystic ovary syndrome (PCOS); homozygous 935A women are at higher risk to develop PCOS. PMID: 24592983
  32. miR-513a-3p is involved in the control of the LHCGR expression by an inversely regulated mechanism at the post-transcriptional level. PMID: 24747085
  33. Data (including data from subjects with mutations) suggest that signaling via LHCGR by luteinizing hormone and chorionic gonadotropin plays physiological roles in human development and pathophysiological roles in reproductive disorders. [REVIEW] PMID: 24283620
  34. during recovery from LH receptor desensitization, LH receptors are both located with DRM lipid environments and confined within small, mesoscale (80-160 nm) cell-surface compartments PMID: 23990106
  35. Although luteinizing hormone and chorionic gonadotropin bind to a common receptor, emerging evidence suggests that they have disparate effects on downstream signaling cascades. (Review) PMID: 24365330
  36. results of the experiments showed that hCGbeta action leads to the increase in ERK and AKT kinases phosphorylation in cancer cells and indicate that these biological effects can be achieved independently of human chorionic gonadotropin presence PMID: 24554412
  37. LHCGR and 17alpha-hydroxylase/17-20-lyase (CYP17A1) protein levels are increased in polycystic ovaries (PCOs). PMID: 24014605
  38. Regarding LHR, three patients were heterozygous for the known variant Glu354Lys and two for Ile374Thr. Intronic polymorphisms were not identified PMID: 23884663
  39. hypomethylation of LHCGR is a potential mechanism underlying susceptibility to polycystic ovary syndrome PMID: 24527662
  40. Examined the mechanisms of receptor signaling, intermolecular cooperation and intramolecular cis-activation. LHR-delExon10 was combined with LHR Lys605-->Glu, in which signaling is abolished, and the LHR mutant Cys131-->Arg, in which binding is deficient. PMID: 24438591
  41. AdipoR2 is regulated by the LH receptor function via a cAMP dependent mechanism. PMID: 23779096
  42. High expression/ activation of GPER, and low expression of LHCGR/FSHR correlate with significantly greatly survival in ovarian epithelial cancer patients. PMID: 23951246
  43. Expression levels of LHR-associated protein are determined in granulosa-luteal cells by real-time reverse-transcription (PCR). PMID: 23433069
  44. Data suggest that physical association of recombinant LHR (lutropin receptor) and FSHR (follitropin receptor) into heterodimeric complexes exhibits a pattern of cross-attenuation of signaling by each receptor and its hormone. PMID: 23825122
  45. A complex neuroendocrine circuit exists in the retina, involving hCG secreting cells, hCG targets and hCG-release controlling cells. PMID: 23285091
  46. case-control study comprising 651 TC cases and 313 controls in a Norwegian population was conducted for investigation of polymorphisms in the LHR, SRD5A and AR genes and their possible association with TC PMID: 22882535
  47. The LHCGR is able to differentiate the activity of luteinizing hormone and chorionic gonadotropin. PMID: 23071612
  48. A novel point mutation of the LHCGR gene has been identified in a family affected with FMPP. The c.1703C>T mutant LHCGR was confirmed to be constitutively active, which has led to maturation and proliferation of Leydig cells. PMID: 23225038
  49. Mutations in LHCGR are relatively rare causes of mild and severe forms of hypergonadotropic hypogonadism or disorders of sex development leading to infertility, both male and female. [REVIEW] PMID: 23044874
  50. The gene products LHCGR and FSHR therefore are likely to be important in the etiology of PCOS, regardless of ethnicity. PMID: 23118426

FAQs

Please fill out the Online Inquiry form located on the product page. Key product information has been pre-populated. You may also email your questions and inquiry requests to sales1@betalifesci.com. We will do our best to get back to you within 4 business hours.

Feel free to use the Chat function to initiate a live chat. Our customer representative can provide you with a quote immediately.

Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

More from High-Quality Recombinant Proteins
Sale
Recombinant Human PARP2 Protein (N-GST & C-6xHis)
  • Molecule: PARP2;
  • Species: Human;
  • Expression system: Baculovirus;
  • Tag: N-terminal GST-tagged and C-terminal 6xHis-tagged;
  • Sequence: Full Length;
CAT#: BLC-11423P

Save $850
Sale
Greater than 85% as determined by SDS-PAGE.
Recombinant Human ACP3 Protein (C-10xHis)
  • Molecule: ACP3;
  • Species: Human;
  • Expression system: Baculovirus;
  • Tag: C-terminal 10xHis-tagged;
  • Sequence: Partial of Isoform 2;
CAT#: BLC-11438P

Save $850
Sale
Greater than 90% as determined by SDS-PAGE.
Recombinant Human MUSK Protein (TBD)
  • Molecule: MUSK;
  • Species: Human;
  • Expression system: in vitro E.coli expression system;
  • Tag: TBD;
  • Sequence: Partial;
CAT#: BLC-11435P

Save $850
Sale
Greater than 90% as determined by SDS-PAGE.
Recombinant Human SLC39A1 Protein (N-GST)
  • Molecule: SLC39A1;
  • Species: Human;
  • Expression system: E.coli;
  • Tag: N-terminal GST-tagged;
  • Sequence: Cytoplasmic Domain;
CAT#: BLC-11425P

Save $850
Sale
Greater than 90% as determined by SDS-PAGE.
Recombinant Human TLR5 Protein (C-6xHis)
  • Molecule: TLR5;
  • Species: Human;
  • Expression system: E.coli;
  • Tag: C-terminal 6xHis-tagged;
  • Sequence: Partial;
CAT#: BLC-11405P

Save $850
Sale
Greater than 85% as determined by SDS-PAGE.
Recombinant Human GPR20 Protein (N-10xHis)
  • Molecule: GPR20;
  • Species: Human;
  • Expression system: in vitro E.coli expression system;
  • Tag: N-terminal 10xHis-tagged;
  • Sequence: Full Length;
CAT#: BLC-11447P

Save $850
Recently viewed