Recombinant Human Leucine-Rich Repeat Flightless-Interacting Protein 1 (LRRFIP1) Protein (His)

Name: Recombinant Human Leucine-Rich Repeat Flightless-Interacting Protein 1 (LRRFIP1) Protein (His)
Brand: Beta LifeScience
SKU: BLC-10276P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Human Leucine-Rich Repeat Flightless-Interacting Protein 1 (LRRFIP1) Protein (His) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	Q32MZ4
Target Symbol	LRRFIP1
Synonyms	FLAP 1; FLAP1; FLIIAP 1; FLIIAP1; GC binding factor 2; GC-binding factor 2; GCF 2; GCF2; HUFI 1; HUFI1; Leucine rich repeat (in FLII) interacting protein 1; Leucine rich repeat flightless interacting protein 1; Leucine-rich repeat flightless-interacting protein 1; LRR FLII interacting protein 1; LRR FLII-interacting protein 1; LRRF1_HUMAN; LRRFIP 1; Lrrfip1; MGC10947; MGC119738; MGC119739; NEDD8 conjugating enzyme; TAR RNA interacting protein; TAR RNA-interacting protein; Transcription factor 9 like; TRIP
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	MTSPAAAQSREIDCLSPEAQKLAEARLAAKRAARAEAREIRMKELERQQKEVEERPEKDFTEKGSRNMPGLSAATLASLGGTSSRRGSGDTSISIDTEASIREIKDSLAEVEEKYKKAMVSNAQLDNEKTNFMYQVDTLKDMLLELEEQLAESRRQYEEKNKEFEREKHAHSILQFQFAEVKEALKQREEMLEKHGIILNSEIATNGETSDTLNNVGYQGPTKMTKEELNALKSTGDGTLGRASEVEVKNEIVANVGKREILHNTEKEQHTEDTVKDCVDIEVFPAGENTEDQKSSEDTAPFLGTLAGATYEEQSLSTCSLDR
Expression Range	1-314aa
Protein Length	Partial of Isoform 3
Mol. Weight	40.1kDa
Research Area	Transcription
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA. Positively regulates Toll-like receptor (TLR) signaling in response to agonist probably by competing with the negative FLII regulator for MYD88-binding.
Subcellular Location	Nucleus. Cytoplasm.
Protein Families	LRRFIP family
Database References	HGNC: 6702 OMIM: 603256 KEGG: hsa:9208 STRING: 9606.ENSP00000375857 UniGene: Hs.471779
Tissue Specificity	Ubiquitously expressed.

Gene Functions References

Results show that LRRFIP-1 is mainly produced by keratinocytes in the unwounded skin but is upregulated in both keratinocytes and fibroblasts in response to wounding, suggesting a role in the wound repair process. PMID: 29996558
Silencing of LRRFIP1 reverses the epithelial-mesenchymal transition via inhibition of the Wnt/beta-catenin signaling pathway. PMID: 26047573
High GCF2 expression is associated with hepatoma. PMID: 24761896
Higher baseline LRRFIP1 expression in human glioblastoma multiforme tissue (n=60) is associated with better prognosis upon later treatment with teniposide. PMID: 24690174
Data indicate that LRRFIP1 knockdown increased resting TNF mRNA level altered the ncRNA abundance at the LRRFIP1 binding region. PMID: 24567534
Eight SNPs (rs7575941, rs3769053, rs11689421, rs3820808, rs11680012, rs3806505, rs6739130, and rs11686141) showed evidence of association with at least two adiposity phenotypes and plasma levels of one marker of inflammation. PMID: 23505185
The FLI-interacting domain of LRRFIP1 forms a classic parallel, homodimeric coiled coil with 10 heptad repeats and 22 helical turns. PMID: 23099021
GCF2/LRRFIP1 plays an important role in colorectal cancer metastasis by regulating RhoA-induced cell adhesion. PMID: 22750095
LRRFIP1 is phosphorylated in response to immunologic stimuli and it is directed to lysosomal structures. PMID: 21102652
An association study of 6 of the 63 genes in 4235 cases and 6379 controls showed a putative association with myocardial infarction for COMMD7 (COMM domain-containing protein 7) and a major deviation from the null hypo thesis for LRRFIP1. PMID: 20833976
GCF2/LRRFIP1 appears to act as a repressor and occupies the -308 site in cells that do not make TNF-alpha. PMID: 16199883
Immediately following lipopolysaccharide stimulation, both LRRFIP2 and Flap-1/LRRFIP1 compete with Fliih for interacting with MyD88 to activate the signaling. PMID: 19265123

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.