Recombinant Human Leucine-Rich Repeat And Immunoglobulin-Like Domain-Containing Nogo Receptor-Interacting Protein 1 (LINGO1) Protein (His)

Name: Recombinant Human Leucine-Rich Repeat And Immunoglobulin-Like Domain-Containing Nogo Receptor-Interacting Protein 1 (LINGO1) Protein (His)
Brand: Beta LifeScience
SKU: BLC-01678P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Human Leucine-Rich Repeat And Immunoglobulin-Like Domain-Containing Nogo Receptor-Interacting Protein 1 (LINGO1) Protein (His) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	Q96FE5
Target Symbol	LINGO1
Synonyms	Leucine-rich repeat and immunoglobulin domain-containing protein 1;Leucine-rich repeat neuronal protein 1;Leucine-rich repeat neuronal protein 6A
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	CPPRCECSAQDRAVLCHRKRFVAVPEGIPTETRLLDLGKNRIKTLNQDEFASFPHLEELELNENIVSAVEPGAFNNLFNLRTLGLRSNRLKLIPLGVFTGLSNLTKLDISENKIVILLDYMFQDLYNLKSLEVGDNDLVYISHRAFSGLNSLEQLTLEKCNLTSIPTEALSHLHGLIVLRLRHLNINAIRDYSFKRLYRLKVLEISHWPYLDTMTPNCLYGLNLTSLSITHCNLTAVPYLAVRHLVYLRFLNLSYNPISTIEGSMLHELLRLQEIQLVGGQLAVVEPYAFRGLNYLRVLNVSGNQLTTLEESVFHSVGNLETLILDSNPLACDCRLLWVFRRRWRLNFNRQQPTCATPEFVQGKEFKDFPDVLLPNYFTCRRARIRDRKAQQVFVDEGHTVQFVCRADGDPPPAILWLSPRKHLVSAKSNGRLTVFPDGTLEVRYAQVQDNGTYLCIAANAGGNDSMPAHLHVRSYSPDWPHQPNKTFAFISNQPGEGEANSTRATVPFPFDIKTLIIAT
Expression Range	42-561aa
Protein Length	Partial
Mol. Weight	63.1 kDa
Research Area	Developmental Biology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Functional component of the Nogo receptor signaling complex (RTN4R/NGFR) in RhoA activation responsible for some inhibition of axonal regeneration by myelin-associated factors. Is also an important negative regulator of oligodentrocyte differentiation and axonal myelination. Acts in conjunction with RTN4 and RTN4R in regulating neuronal precursor cell motility during cortical development.
Subcellular Location	Cell membrane; Single-pass type I membrane protein.
Database References	HGNC: 21205 OMIM: 609791 KEGG: hsa:84894 STRING: 9606.ENSP00000347451 UniGene: Hs.656765
Tissue Specificity	Expressed exclusively in the central nervous system. Highest level in the in amygdala, hippocampus, thalamus and cerebral cortex. In the rest of the brain a basal expression seems to be always present. Up-regulated in substantia nigra neurons from Parkins

Gene Functions References

We identified homozygous missense variants in LINGO1, p.(Arg290His) in family F162 and p.(Tyr288Cys) in family PKMR65. Both variants were predicted to be pathogenic, and segregated with the phenotype in the respective families. Molecular modeling of LINGO1 suggests that both variants interfere with the glycosylation of the protein. PMID: 28837161
LINGO1 rs11856808 plays a protective role by decreasing the risk for PD, but not for MSA, in Chinese population. PMID: 26254004
LINGO1 protein acts as a gateway protein internalizing into the tumor cells when engaged by antibody and can carry antibody conjugated with drugs to kill Ewing sarcoma cells. PMID: 26979953
The results of this study show Increased LINGO1 in the cerebellum of essential tremor patients. PMID: 24531928
Lingo-1 signaling is altered in the schizophrenia brain. PMID: 24448210
LINGO-1 can directly bind to ErbB2, block ErbB2 translocation into lipid rafts, and inhibit its phosphorylation. PMID: 24583087
This review found that several gene variants in the LINGO1 gene may increase the risk of essential tremor. PMID: 23682623
LINGO1 variants are associated with essential tremor in Chinese Han female patients. PMID: 23754655
This study demonistrated that theLINGO1 rs9652490 and rs11856808 polymorphisms are not associated with risk for multiple sclerosis. PMID: 23574883
Results of a meta-analysis suggest a relationship between LINGO1 rs11856808 polymorphism and risk of essential tremor (ET) and familial ET, while rs9652490 polymorphism is only associated with the risk for familial ET. PMID: 22425540
the N-terminal region containing the leucine-rich repeats along with the transmembrane and cytoplasmic domains of LINGO-1 are not required for self-interaction or interaction with amyloid precursor protein PMID: 22133804
the present meta-analysis does not support the notion that LINGO1 rs9652490 SNP is a major genetic risk factor for parkinson disease.[meta-analysis] PMID: 22710005
This study showed that LINGO1 rs9652490 and rs11856808 are not associated with the risk of Parkinson's disease. PMID: 21955595
LINGO-1, a transmembrane signaling protein, inhibits oligodendrocyte differentiation and myelination through intercellular self-interactions PMID: 22514275
There were no significant differences in frequencies for all alleles between the essential tremor (ET) group and controls; however, an association of genotype A/G of the SNP rs9652490 with the familial ET phenotype was found. PMID: 21741293
Single nucleotide polymorphism(SNP)s of LINGO1 play a role in the development of Parkinson's disease in the Italian population. PMID: 21752692
LINGO1 variants are not a major risk factor for developing familial essential tremor in this population, which suggests the existence of other genetic risk factors responsible for familial essential tremor in this movement disorder clinic population. PMID: 21219542
while there were no significant differences in the Lingo1 minor allele frequency and genotype frequency between Chinese essential tremor and controls, pooled analysis showed a higher proportion of GG genotype in tremor patients PMID: 21158743
No significant differences are found in genotype and allele distribution in the rs9652490 variant of LINGO1 in either Chinese or Caucasian Parkinson disease patients. PMID: 20951767
This study demonistrated that LINGO1 variants could increase risk of PD, specifically those presenting the non-rigid-akinetic phenotypes, which suggests that LINGO1 may have a role in the etiology of tremor in PD at least in the Spanish population. PMID: 21506150
genotyping results lead us to conclude that no association exists between the key variant rs9652490 and Essential tremor (P(corr) = 1.00). PMID: 21264305
LINGO1 variant rs9652490 (A > G) is unlikely to play a major role in Parkinson's disease in Chinese populations. PMID: 20957646
LINGO-1 inhibits multiple aspects of oligodendrocyte differentiation independently of the LRRs via a process that requires p75(NTR) signalling PMID: 20659559
LINGO1 and LINGO2 variants are associated with essential tremor and Parkinson disease PMID: 20369371
The two markers rs9652490 and rs11856808 were not strongly related to the essential tremor,late onset sporadic Parkinson's disease, but the rs9652490G allele might be a protective factor for the early onset Parkinson's disease in Chinese population. PMID: 20600614
The LINGO1 gene is a risk factor for essential tremor in a Caucasian population in North America. PMID: 20372186
Our study gives further evidence that LINGO1 acts as a susceptibility gene for essential tremor. PMID: 20310002
Thisdy demonstrateda significant association between LINGO1 rs9652490 and essential tremor (P = 0.014) and Parkinson disease (P = 0.0003), thus providing the first evidence of a genetic link between both diseases. PMID: 19720553
LINGO1 SNP (rs9652490) is not associated with sporadic PD in our Polish cohort. PMID: 20117178
In this study, SNPs rs9652490, rs11856808, and rs7177008 of LINGO1 were genotyped in a total of 694 Austrian subjects (349 PD, 345 controls). No association could be found between genotype or allele counts and Parkinson disease. PMID: 19908305
The LERN1 expression pattern is specific to the central nervous system, highly and broadly expressed during early stages of development and gradually restricted to forebrain structures as development proceeds(LERN1) PMID: 14686891
the Lingo-1 ectodomain is a module implicated in central nervous system repair inhibition PMID: 17005555
inhibitory agents of LINGO-1 activity can protect dopaminergic neurons against degeneration PMID: 17726113
Variant in the sequence of the LINGO1 gene confers risk of essential tremor. PMID: 19182806
Quick identification of essential N-glycosylation sites of a heavily glycosylated neuroglycoprotein Lingo-1, which are sufficient for the support of its surface expression. PMID: 19254717
the LINGO1 gene is associated with an increased risk for essential tremor PMID: 19553813
LINGO1 variant increases risk of familial essential tremor. PMID: 19805735

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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