Recombinant Human LAMP1 Protein (C-6His)

Name: Recombinant Human LAMP1 Protein (C-6His)
Brand: Beta LifeScience
SKU: BL-1225NP
Availability: InStock

BL-1225NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Lysosome-Associated Membrane Glycoprotein 1 is produced by our Mammalian expression system and the target gene encoding Ala29-Met382 is expressed with a 6His tag at the C-terminus.
Accession	P11279
Synonym	Lysosome-Associated Membrane Glycoprotein 1; LAMP-1; Lysosome-Associated Membrane Protein 1; CD107 Antigen-Like Family Member A; CD107a; LAMP1
Gene Background	Lysosome-Associated Membrane Glycoprotein 1 (LAMP1) is a single-pass type I membrane protein belonging to the LAMP family. LAMP1 is expressed largely in the endosome-lysosome membranes of cells.It shuttles between lysosomes, endosomes, and the plasma membrane. LAMP1 functions to present carbohydrate ligands to selectins and it has also been implicated in tumor cell metastasis. It has been proposed LAMP1 can be used as a therapeutic agent for certain cancers, as well as a marker for lysosomal storage disorders and degranulation on lymphocytes such as CD8+ and NK cells. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells(PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMCs to the site of inflammation.
Molecular Mass	39.42 KDa
Apmol Mass	55-120 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, autophagy, and cholesterol homeostasis. Plays also an important role in NK-cells cytotoxicity. Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule. In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content. Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.; (Microbial infection) Acts as a receptor for Lassa virus glycoprotein. Promotes also fusion of the virus with host membrane in less acidic endosomes.; (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F.
Subcellular Location	Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Late endosome membrane; Single-pass type I membrane protein. Cytolytic granule membrane; Single-pass type I membrane protein.
Protein Families	LAMP family
Database References	HGNC: 6499 OMIM: 153330 KEGG: hsa:3916 STRING: 9606.ENSP00000333298 UniGene: PMID: 29749495 A novel data on LAMP-1 overexpression in high grade glioma are presented suggesting involvement of this gene and protein in cell adhesion and tumor progression. PMID: 29920782 LAMP1 is involved in the TNM stages and histological differentiation of the esophageal squamous cell carcinoma PMID: 28687162 LAMP1 has a role in fratricide amongst T cell receptor transgenic CD8+ T cells directed against tumor-associated antigens PMID: 27447745 Impaired autophagic pathways were further shown by analyzing late autophagic vesicles; immunostaining with lysosome-associated membrane protein 1 (LAMP-1) antibody revealed enlarged and annular LAMP-1-positive organelles in AMD retinal pigment epithelium (RPE)as opposed to smaller discrete puncta observed in normal RPE PMID: 28055007 The authors show here that human LAMP1 and LAMP2 bind cholesterol in a manner that buries the cholesterol 3beta-hydroxyl group; they also bind tightly to NPC1 and NPC2 proteins that export cholesterol from lysosomes. PMID: 27664420 downregulation of FUT1, which leads to the perinuclear localization of LAMP-1 and 2, is correlated with increased rate of autophagic flux by decreasing mTOR signaling and increasing autolysosome formation. PMID: 27560716 the assembly modes of LAMP-1 and LAMP-2 are different, which may underlie their distinct functions PMID: 27663661 Overexpression of LAMP1 was observed in prostate cancer (PCa) and castration-resistant prostate cancer clinical specimens. Moreover, downstream pathways were identified using si-LAMP1-transfected cells. The discovery of tumor-suppressive miR320a-mediated pathways may provide important insights into the potential mechanisms of PCa metastasis. PMID: 27212625 The data implied that high LAMP1 expression is associated with unfavourable prognosis in laryngeal squamous cell carcinoma patients, and LAMP1 may be identified as a novel prognostic biomarker PMID: 27788920 The data support a viral entry mechanism dependent on binding to the lysosome-resident receptor LAMP1 and further dissociation of the membrane-distal GP1 subunits. PMID: 26849049 A unique triad of histidines of Lassa Virus GP1 forms a binding site for host LAMP1. PMID: 25972533 The LAMP-1 had preferential localization in the high density secondary lysosomes where endogenous human LAMP-1 was enriched. In contrast, a major portion of I382L was located in a low density fraction. PMID: 24695761 Downregulation of miR-184 was consistent with significantly lower levels of LAMP-1 PMID: 25251993 Synergistic defects of different molecules in the cytotoxic pathway lead to clinical familial hemophagocytic lymphohistiocytosis. PMID: 24916509 Caveolin-1 associated adenovirus entry into human corneal cells. PMID: 24147000 study has shown that Lassa virus entry requires a pH-regulated engagement of alpha-DG and LAMP1 both of which need to be glycosylated PMID: 24970085 in TB pleurisy regulatory T lymphocytes effectively inhibit CD107a expression PMID: 24134738 LAMP-1 and LAMP-2 could be used as additional markers with which to assess enzyme replacement therapy effectiveness in Fabry disease. PMID: 24334114 LAMP-1/GFAP showed pronounced co-expression and LAMP-1/CD133 was co-expressed as well in astrocytomas suggesting that tumor cells including the proposed tumor stem cells contain lysosomes. PMID: 23826410 CD107a/LAMP-1 has a role in the protection of NK cells from degranulation-associated suicide PMID: 23847195 Data indicate that monoclonal antibodies specific to CD107a (LAMP-1) or CD107b (LAMP-2) enhanced LPS-induced IL-8 secretion of THP-1 cells. PMID: 23603048 LAMP1 is required for efficient perforin delivery to lytic granules and NK-cell cytotoxicity. PMID: 23632890 Simultaneous expression of LAMP1 and EGFR correlates with the ovarian neoplasm grading. PMID: 23172893 LAMP proteins retain TAPL on the limiting membrane of endosomes and prevent its sorting to intraluminal vesicles. PMID: 22641697 Data indicate that lysosomally targeted cameleon Ca2+ probe LAMP1-YCaM accurately and sensitively responds to both global and lysosome-specific Ca2+ signals. PMID: 23098255 Report expression of NKG2D and CD107 in CD8(+) effector memory lymphocytes in Churg-Strauss syndrome. PMID: 22640649 nucleolin and LAMP-1 have a role in promoting infection of human monocytes by Francisella tularensis PMID: 21152024 Human full-length amelogenin increases the proliferation of mesenchymal stem cells by interaction with LAMP1 through the MAPK-ERK signaling pathway. PMID: 20967466 Immunohistochemistry studies showed EGBs to exhibit pronounced reactivity to antibodies against lysosome-associated membrane proteins (LAMP)-1 and LAMP-2, and the lysosomal enzyme cathepsin D. PMID: 20926008 review of structure, function, cell and tissue distribution, intracellular trafficking PMID: 12144129 This study identifies two cDNA clones that code for heart extracellular matrix protein, hLAMP-1. Immunohistochemical studies indicate that the myocardial layer of the heart produces hLAMP-1. PMID: 15052658 Using human leukemia and lymphoma cell lines, we observed a close correlation between CD107a surface expression and target cell lysis, indicating that NK cell cytotoxicity can be assessed by this method. PMID: 15744340 Data identify CD13, CD107a, and CD164 as novel basophil-activation antigens. PMID: 15916720 Novel evidence for differential expression of HBGA and LAMPs in proliferative and involutive phases of immunohistochemistry is presented. PMID: 16570122 expression in keratinocytes cultured at different cell densities in order to induce differentiation PMID: 16710742 Enhanced expression of lysosome-associated membrane protein-1 in melanoma may promote invasion by influencing both adhesion to extracellular matrix and perhaps also binding to endothelial cells. PMID: 16718270 CD107a surface expression has a role in Munc13-4 defect in familial hemophagocytic lymphohistiocytosis PMID: 16778144 LAMP-1 and DC-LAMP antigen chimeras follow different trafficking pathways, induce distinct modulatory immune responses, and are able to present cryptic epitopes. PMID: 16887987 LAMP-1 and LAMP-2 may have roles in accidental involution of the thymic gland PMID: 17048695 Data show that cells lacking either LAMP-1 or LAMP-2 alone formed phagosomes that gradually acquired microbicidal activity and curtailed bacterial growth, but LAMP-1 and LAMP-2 double-deficient cells failed to kill engulfed Neisseria gonorrhoeae. PMID: 17506821 CD107a expression may be a sensitive marker for the cytotoxic activity determination PMID: 18835598 Despite its abundance, LAMP-1 is not essential, but LAMP-2 may be partially important for the Salmonella-containing vacuolar membrane. PMID: 18958159 the activation-induced degranulation of Fas ligand has distinct requirements and involves different mechanisms than those of the granule markers CD63 and CD107a/Lamp-1 PMID: 19079288 Most Hassal's corpuscules in thymoma were negative for LAMPs, but positive in normal thymus. Both lymphocytes and epithelial cells in pathological thymus showed higher intensity for LAMP-2 compared with LAMP-1. PMID: 19343823 Data show that The cell surface expression levels of (ICAM)-2 and -3 on the apoptotic cells were markedly lower, while those of calnexin, calreticulin, and (LAMP)-1 and -2 were significantly higher compared to non-apoptotic cells. PMID: 19524015

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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