Recombinant Human Keratin, Type Ii Cytoskeletal 8 (KRT8) Protein (His-SUMO)

Name: Recombinant Human Keratin, Type Ii Cytoskeletal 8 (KRT8) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-03058P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Keratin, Type Ii Cytoskeletal 8 (KRT8) Protein (His-SUMO) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P05787
Target Symbol	KRT8
Synonyms	CARD2; CK 8; CK-8; CK8; CYK8; CYKER; Cytokeratin endo A; Cytokeratin-8; DreK8; EndoA; K2C8; K2C8_HUMAN; K8; Keratin 8; Keratin type II cytoskeletal 8 ; Keratin; type II cytoskeletal 8; Keratin-8; KO; Krt 2.8; KRT8; MGC118110; MGC174782; MGC53564; MGC85764; sb:cb186; Type-II keratin Kb8
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	SIRVTQKSYKVSTSGPRAFSSRSYTSGPGSRISSSSFSRVGSSNFRGGLGGGYGGASGMGGITAVTVNQSLLSPLVLEVDPNIQAVRTQEKEQIKTLNNKFASFIDKVRFLEQQNKMLETKWSLLQQQKTARSNMDNMFESYINNLRRQLETLGQEKLKLEAELGNMQGLVEDFKNKYEDEINKRTEMENEFVLIKKDVDEAYMNKVELESRLEGLTDEINFLRQLYEEEIRELQSQISDTSVVLSMDNSRSLDMDSIIAEVKAQYEDIANRSRAEAESMYQIKYEELQSLAGKHGDDLRRTKTEISEMNRNISRLQAEIEGLKGQRASLEAAIADAEQRGELAIKDANAKLSELEAALQRAKQDMARQLREYQELMNVKLALDIEIATYRKLLEGEESRLESGMQNMSIHTKTTSGYAGGLSSAYGGLTSPGLSYSLGSSFGSGAGSSSFSRTSSSRAVVVKKIETRDGKLVSESSDVLPK
Expression Range	2-483aa
Protein Length	partial
Mol. Weight	66.5 kDa
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Subcellular Location	Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
Protein Families	Intermediate filament family
Database References	HGNC: 6446 OMIM: 148060 KEGG: hsa:3856 STRING: 9606.ENSP00000293308 UniGene: PMID: 29443941 K8/K18 variants are overrepresented in Chinese NAFLD patients and might accelerate liver fat storage through insulin resistance. PMID: 28652657 regulated enhancement of autophagy with concurrent increased expression of KRT8 and the inhibition of KRT8 phosphorylation serve to inhibit oxidative stress-induced epithelial-mesenchymal transitionof retinal pigment epithelium cells. PMID: 28045574 Histopathological differences between Magnetic resonance-guided high-intensity focused ultrasound (MR-HIFU)-ablated tissue and radiofrequency (RF)-ablated tissue were demonstrated. CK-8 was more reliable for cell viability assessment than H&E when used directly after ablation PMID: 26732321 Loss of activating transcription factor 3 (ATF3) in knockout mice promotes the emergence of keratins CK5+CK8+ epithelial cells. PMID: 26522727 notochord-specific marker during early intervertebral disc development PMID: 26910849 Data show that the filament elongation of both desmin and keratin K8/K18 proceeds very similar to that of vimentin. PMID: 27304995 New insights into interactions between the nucleotide-binding domain of CFTR and keratin 8. PMID: 27870250 We present results of a multicentre study measuring UBC((R)) Rapid Test(test that detects fragments of cytokeratins 8 and 18 in urine) in bladder cancer patients and healthy controls with focus on carcinoma in situ (CIS) and high-grade bladder cancer. PMID: 28468590 Data show that after siRNA transfection, TGF-beta1 protein level was decreased, and CK18 proteins was decreased, while CK8 proteins was increased, and TERT protein expression was slightly increased at 96 h. PMID: 26462837 The interplay between Solo protein and keratin 8/keratin 18 filaments plays a crucial role in tensile force-induced RhoA activation and consequent actin cytoskeletal reinforcement in endothelial cells. PMID: 26823019 K8/18 filaments provide resistance to apoptosis in granulosa cell tumor cells by impairing FAS expression. PMID: 26911253 These metastatic tumors revealed no detectable expression of CK8/18, E-cadherin, VCAM-1, and ICAM-1 PMID: 26847082 Data show that loss of epithelial membrane protein 2 (EMP2) is involved in sphingosylphosphorylcholine (SPC)-induced phosphorylation of keratin 8 (K8) via ubiquitination of protein phosphatase 2 (PP2A) through alpha4 phosphoprotein by caveolin-1 (cav-1). PMID: 26876307 The reactivity of rheumatoid arthritis (RA) serum IgG antibodies against K8 was significantly higher than healthy controls and systemic lupus erythematosus patients, confirming K8 as a novel autoantigen of RA. PMID: 26277397 Variants in highly conserved residues of K8 Lys393Arg (ezetimibe/simvastatin-related) was found in patients with fatal Drug-induced liver injury. PMID: 26286715 Keratins 8 and 18 are type II acute-phase responsive genes overexpressed in human liver disease. PMID: 24930437 In human failing myocardium, where TNF-alpha expression is upregulated, K8/K18 were also ectopically expressed PMID: 26280121 Human KRT8 variants promote acetaminophen induced hepatotoxicity in mouse models. PMID: 25963979 Syndecan-1 is involved in morphogenesis of the developing tooth crown and cervical loops, andntogether with CK8 and vimentin in differentiation of preameloblasts and preodontoblasts. PMID: 25120060 K8 immunostaining of tissue from the lymph node metastasis allows defining a sub-group of lymph node-positive breast cancer patients with a highly unfavourable outcome PMID: 25724181 This is the first time intestinal cells have been suggested also functionally impaired by K8/K18 mutations. PMID: 24915158 Study indicates that Piwil2 plays a role in Fas-mediated apoptosis and can affect p53 phosphorylation in tumor cells, revealing a novel mechanism of Piwil2 in apoptosis, and supports that Piwil2 plays an active role in tumorigenesis. PMID: 25113562 An increased expression of cytokeratin 8 and weak of calponin in tunica media of saphenous vein grafts might be useful markers of unfavorable long-term prognosis in CABG patients. PMID: 24338534 These data support the idea that CK8 is an important receptor for Pet on epithelial cells for starting its cytotoxic effects. PMID: 24327340 parkin may inhibit cell growth, at least in part, by decreasing RPSA expression and inducing phosphorylation of cytokeratin 8/18 PMID: 23990477 These findings, along with the emerging role of cell surface CK8 in cancer metastasis, may imply a dual strategy for tumor cell survival in the host. PMID: 24183726 CK8 up-regulation might have a functional role in HCV infection and pathogenesis, and its ectopic expression induces apoptosis of SMMC7721 cells. PMID: 24115814 this is the first study to show a down-regulated CK8 expression and the percentage of CK8 positive cell clusters in IDD based upon multiple lines of evidence PMID: 23801880 Compressive loads resulted in phosphorylation and disassembly of CK8 in nucleus pulposus cells. PMID: 24166186 A conserved rod domain phosphotyrosine that is targeted by the phosphatase PTP1B promotes keratin 8 protein insolubility and filament organization. PMID: 24003221 These results indicate the role of K8/18 in modulating invasion in breast cancer. PMID: 23341946 Report keratin 8 expression in breast papillomas and papillary carcinoma. PMID: 23327593 K8/18 expression can influence the phenotype of epithelial cancer cells at a transcriptional level and supports the hypothesis that keratins play an active role in cancer progression. PMID: 23449973 Keratin variants are expressed in a low percentage of patients with alcoholic cirrhosis and do not influence hepatocellular carcinoma development. PMID: 23078008 There is aberrant expression of CK8 in dysplasia, oral submucous fibrosis, and oral squamous cell carcinoma, suggesting CK8 as a surrogate marker of malignant transformation. PMID: 22677743 K8 acetylation at Lys-207, a highly conserved residue among type II keratins, is up-regulated upon hyperglycemia and down-regulated by SIRT2. PMID: 23358244 A key K8/K18 regulation of glucose metabolism in normal and cancerous hepatic cells through differential modulations of mitochondrial hexokinase status and insulin-mediated signaling. PMID: 23164509 Fibroblasts prepared from different types of malignant tumors stimulate expression of luminal marker keratin 8. PMID: 22270320 Keratin filaments were assembled from recombinant keratin proteins 8 (K8) and 18 (K18) in the presence of divalent ions (Mg(2+)). PMID: 22853896 Keratin 8 phosphorylation regulates keratin reorganization and migration of epithelial tumor cells. PMID: 22344252 Results show K8 is present on the cell surface of transformed prostate cancer cell lines, and K8 increased the activity of plasmin approximately fivefold over a 48-h period. K8 also enhanced the plasmin-mediated proteolysis of vitronectin. PMID: 22354665 In patients with hemochromatosis, intronic but not exonic K8/K18 variants associate with liver fibrosis development. PMID: 22412904 We have found the moderate positive correlation between proliferation and a keratin 8 expression in neoplastic epithelial cells. PMID: 22379894 previously undescribed K8 variants associate with cryptogenic hepatitis in a German cohort of patients, possibly predisposing carriers to the development of liver disease. PMID: 22419260 Loss of keratin 8 phosphorylation leads to increased oral squamous cell carcinoma progression PMID: 22114688 Suggest that CK8 is unsuitable for early diagnosis of tongue squamous cell carcinoma. PMID: 21705376 the mild KRT8 allele is associated with CFTR mediated residual chloride secretion among F508del-CFTR homozygotes PMID: 21548936 The study demonstrates the strong expression of CK8 in limbal epithelial basal cells, which is maintained during the differentiation and migration of the limbal cells toward the central corneal epithelium. PMID: 20926822 Clumping factor B is capable of binding cytokeratin 8 during infection. PMID: 20817646

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.