Recombinant Human Keratin, Type I Cytoskeletal 18 (KRT18) Protein (His)

Name: Recombinant Human Keratin, Type I Cytoskeletal 18 (KRT18) Protein (His)
Brand: Beta LifeScience
SKU: BLC-04835P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Keratin, Type I Cytoskeletal 18 (KRT18) Protein (His) is produced by our Mammalian cell expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P05783
Target Symbol	KRT18
Synonyms	Cell proliferation inducing gene 46 protein; Cell proliferation inducing protein 46 ; Cell proliferation-inducing gene 46 protein; CK 18; CK-18; CK18; CYK 18; CYK18; Cytokeratin 18; Cytokeratin endo B; Cytokeratin-18; K 18; K18; K1C18_HUMAN; KA18; Keratin 18; Keratin 18, type I; Keratin D ; keratin, type I cytoskeletal 18; Keratin-18; Krt18
Species	Homo sapiens (Human)
Expression System	Mammalian cell
Tag	N-10His
Target Protein Sequence	SFTTRSTFSTNYRSLGSVQAPSYGARPVSSAASVYAGAGGSGSRISVSRSTSFRGGMGSGGLATGIAGGLAGMGGIQNEKETMQSLNDRLASYLDRVRSLETENRRLESKIREHLEKKGPQVRDWSHYFKIIEDLRAQIFANTVDNARIVLQIDNARLAADDFRVKYETELAMRQSVENDIHGLRKVIDDTNITRLQLETEIEALKEELLFMKKNHEEEVKGLQAQIASSGLTVEVDAPKSQDLAKIMADIRAQYDELARKNREELDKYWSQQIEESTTVVTTQSAEVGAAETTLTELRRTVQSLEIDLDSMRNLKASLENSLREVEARYALQMEQLNGILLHLESELAQTRAEGQRQAQEYEALLNIKVKLEAEIATYRRLLEDGEDFNLGDALDSSNSMQTIQKTTTRRIVDGKVVSETNDTKVLRH
Expression Range	2-430aa
Protein Length	Full Length of Mature Protein
Mol. Weight	50.8
Research Area	Signal Transduction
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Subcellular Location	Cytoplasm, perinuclear region. Nucleus, nucleolus.
Protein Families	Intermediate filament family
Database References	HGNC: 6430 OMIM: 148070 KEGG: hsa:3875 STRING: 9606.ENSP00000373487 UniGene: PMID: 29437899 This study analyzed phase III data comparing basal insulin peglispro (BIL) and insulin glargine in type 1 (T1D), and type 2 diabetes (T2D) (insulin-naive and insulin-treated). Alanine aminotransferase (ALT), K-18, enhanced liver fibrosis scores and liver fat content. PMID: 29167192 Results found that CK18, MMP-9, and TIMP1 averages of those with positive clinical lymph nodes and those in clinical stage 3 were higher than the averages of those with negative clinical lymph nodes and those in clinical stage 2. PMID: 29651326 non-surviving severe malignant middle cerebral artery infarction patients had higher serum CCCK-18 levels than surviving patients PMID: 29573748 Plasma M30-M65 levels are higher in serum of patients with placental abruption. PMID: 27893299 results show that the caspase digestion-resistant K18 helps to maintain keratin filament organization and delays apoptosis, thereby resulting in protection from liver injury. PMID: 28606991 The plasma keratin-18 (K18) values of nonsurviving alcoholic hepatitis (AH) patients were significantly elevated above their surviving counterparts and healthy controls PMID: 28770701 Evidence suggested that the modification of histone H3 was highly correlated with the modulation of cytokeratin 18 and probably plays an important role in tumorigenesis of hepatocytes PMID: 28647696 K8/K18 interact with Notch1 and regulate Notch1 signalling activity during differentiation of the colonic epithelium. PMID: 28475172 FIB-4 index and CK-18F have good diagnostic abilities not only for nonalcoholic steatohepatitis (NASH) overall, but also for NASH with mild fibrosis. PMID: 29040984 In children with nonalcoholic fatty liver disease, CK18 levels were found to be significantly higher in subjects with any fibrosis compared with those without fibrosis (304.6 +/- 124.8 vs 210.4 +/- 70.9, P < 0.001). PMID: 26835904 CK18 combined with uric acid measurement is a promising non-invasive biomarker for prediction of disease severity in NASH patients PMID: 28472039 Results suggested that serum M65 is a useful indicator of liver inflammation in chronic hepatitis B patients. Serum M65, not M30, is valuable in the grading of liver fibrosis. PMID: 28514295 The results suggest that increased circulating CK-18 and FGF-21 are associated with Nonalcoholic Fatty Liver Disease and may be used for initial assessment, but not enough PMID: 28326329 The purpose of this study is to investigate the immunohistochemical expression of cytokeratin 18 (CK18) and the reactivity to GDF5 (CDMP-1), called the morphogenetic protein-1, cartilage-derived, in lingual squamous cell carcinoma. PMID: 27151703 In patients with anterior STEMI treated with primary PCI, the apoptosis marker M30 might be useful for predicting LV remodeling and subsequent LV systolic dysfunction. PMID: 27479531 Serum concentrations of CK-18 fragments and transgelin-2 correlate with the severity of NAFLD, but not with obesity. PMID: 27740519 High KRT18 serum levels are associated with non-small-cell lung cancer. PMID: 27468866 Serum HGF and CK18 levels were significantly up-regulated and positively correlated with metastasis stage, tumor stage, and disease stage of esophageal squamous cell carcinoma. PMID: 27706656 CK18 plays an important role in lung cancer progression and may be a therapeutic target for non small cell lung cancer. PMID: 27601168 these results suggest that cytokeratin 18 critically contributes to initiating TGF-beta1-induced EMT via the smad 2/3-mediated regulation of snail and slug expression in breast epithelial cells. PMID: 27734227 Study demonstrated that the downregulation of CK18 is associated with the acquisition of paclitaxel-resistance and tumor aggressiveness in prostate cancer. PMID: 26892177 Data indicate that O-GlcNAcylation at serine Ser30 determines the occurrence of Pposphorylation at Ser33 on Keratin 18 (K18). PMID: 27059955 Data show that adipose-derived stem cells (ADSC) differentiate into epithelial-like cells with the expression of epithelial surface marks cytokeratin 18 and 19 and form tube-like structures. PMID: 26416346 High circulating CCCK-18 levels were associated with injury severity and a poor clinical outcome after aSAH and CCCK-18 had the potential to be a good prognostic biomarker for aSAH. PMID: 26671131 NAFLD patients, serum CK-18 levels reflect disease activity scores and correlate with histological changes. PMID: 26436355 Data show that the decrease of keratin 18 (K18) ser33 and ser52 phosphorylation inhibited autophagy and decreased apoptosis of HCT116 cells. PMID: 26728375 As an apoptosis-related biomarker, serum M30 [M30 cytokeratin-18 peptide, human ] level has a merit to be the most promising test for prediction or differential diagnosis of HELLP syndrome in PE patients. PMID: 26138305 Serum caspase-cleaved cytokeratin-18 levels are associated with 30-day mortality and could be used as a prognostic biomarker in patients with severe traumatic brain injury. PMID: 25822281 The results suggest that oblongifolin C inhibits metastasis through the induction of the expression of keratin 18 and may be useful in cancer therapy. PMID: 25973684 Our results suggest that reliable detection of apoptosis via the M30 assay only works when sufficient levels of cytokeratin 18 are present in the cells. This means that the M30 assay may result in false negative results for apoptosis PMID: 25846732 No evidence of association between polymorphisms in KRT18 and sporadic amyotrophic lateral sclerosis in Han Chinese PMID: 25677198 Variants in highly conserved residues of K18 Asp89His (isoniazid-related) were found in patients with fatal Drug-induced liver injury. PMID: 26286715 Serum caspase-cleaved cytokeratin-18 levels were associated with mortality in severe septic patients. PMID: 25290885 Although only serum M65 levels were found to be of diagnostic value, neither M30 nor M65 serum levels played a prognostic role in the outcome in melanoma patients. PMID: 23812330 Under the present study design propofol or sevoflurane anaesthesia did not induce apoptosis or affected the liver function as assessed by the M30, M65 markers and liver enzymes in patients undergoing mastectomy or thyroidectomy under general anaesthesia. PMID: 25579144 There were no statistically significant correlations between serum M30 and M65 concentrations and clinicodemographical variables. Serum M30 and M65 concentrations were found to have a diagnostic value in nasopharyngeal cancer PMID: 25326440 The broken dialog between ANXA1 and CK18 in normal breast tissues may play a critical role in breast cancer development. PMID: 25028131 The distribution of CK18 in a large cohort of older people with type 2 diabetes is associated with an adverse metabolic risk factor profile. PMID: 24237940 placental expression significantly increased in preeclampsia compared to normotensive pregnancies PMID: 24857367 Full length and caspase cleaved cytokeratin 18 (detected as M65 and M30 antigens) are associated with prognosis in hepatitis B virus-related acute-on-chronic liver failure. PMID: 24605039 KRT18 expression is directly regulated by EGR1, and contributes to decrease malignancy of non-small cell lung carcinoma. PMID: 24990820 we identified four proteins with different expression in paclitaxel resistant cells, i.e., serpin B3, serpin B4, heat shock protein 27 (all three upregulated) and cytokeratin 18 (downregulated). PMID: 24898082 effect between the CK-18 concentration and the severity of NAFLD was found in the T2DM patients PMID: 24667697 Due to promising results of various studies, CK-18 cell death markers could be applied in clinical routine soon. PMID: 24622872 Median serum concentration of M30-CK18 was higher in chronic hepatitis C patients and was associated with inflammatory activity and liver fibrosis. PMID: 23981197 A new model that combines ALT, platelets, CK-18 fragments, and triglycerides was established by logistic regression among nonalcoholic fatty liver disease patients. PMID: 24324749 Serum M65 has the potential as a new diagnostic parameter for hepatocellular carcinoma (HCC) and serum CD163 is a new prognostic parameter in HCC patients. PMID: 23792028 M 30 serum levels increase in patients with active ulcerative colitis. PMID: 23945185 These results provide an explanation for the elevated serum levels of HSP-27, HSP-70, and ccCK-18 found in COPD patients, indicating that hypoxic conditions can trigger the release of the aforementioned factors. PMID: 24660549

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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