Recombinant Human Interleukin-10 Receptor Subunit Alpha (IL10RA) Protein (His&Myc)

Name: Recombinant Human Interleukin-10 Receptor Subunit Alpha (IL10RA) Protein (His&Myc)
Brand: Beta LifeScience
SKU: BLC-07729P
Price: 549.6 USD
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: Cytokines, Cytokines and growth factors, Interleukins and receptors, Recombinant proteins

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Product Overview

Description	Recombinant Human Interleukin-10 Receptor Subunit Alpha (IL10RA) Protein (His&Myc) is produced by our Yeast expression system. This is a protein fragment.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	Q13651
Target Symbol	IL10RA
Synonyms	CDw210a Interleukin-10 receptor subunit 1 CD_antigen: CD210
Species	Homo sapiens (Human)
Expression System	Yeast
Tag	N-10His&C-Myc
Target Protein Sequence	HGTELPSPPSVWFEAEFFHHILHWTPIPNQSESTCYEVALLRYGIESWNSISNCSQTLSYDLTAVTLDLYHSNGYRARVRAVDGSRHSNWTVTNTRFSVDEVTLTVGSVNLEIHNGFILGKIQLPRPKMAPANDTYESIFSHFREYEIAIRKVPGNFTFTHKKVKHENFSLLTSGEVGEFCVQVKPSVASRSNKGMWSKEECISLTRQYFTVTN
Expression Range	22-235aa
Protein Length	Partial
Mol. Weight	28.7 kDa
Research Area	Immunology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well. In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively. These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy.
Subcellular Location	Cell membrane; Single-pass type I membrane protein. Cytoplasm.
Protein Families	Type II cytokine receptor family
Database References	HGNC: 5964 OMIM: 146933 KEGG: hsa:3587 STRING: 9606.ENSP00000227752 UniGene: Hs.504035
Associated Diseases	Inflammatory bowel disease 28 (IBD28)
Tissue Specificity	Primarily expressed in hematopoetic cells including B-cells, T-cells, NK cells, monocytes and macrophages. Not expressed in non-hematopoetic cells such as fibroblasts or endothelial cells.

Gene Functions References

This case report and case-control study explores the role of IL10RA and its deficiency in the development of the immune system and inflammatory bowel diseases. PMID: 28644354
We identified 32 compound heterozygous mutations and 9 homozygous mutations in IL10 receptor subunit alpha and 1 homozygous mutation in IL10 receptor subunit beta. Among these mutations, 10 novel mutations were identified, and 6 pathogenic mutations had been previously described. In patients with IL10 receptor subunit alpha mutations, c.301C>T (p.R101RW) and c.537 G>A (p.T179T) were the most common mutations. PMID: 28267044
these findings provide a novel mechanism by which microbial-derived butyrate promotes barrier through IL-10RA-dependent repression of claudin-2 PMID: 28893958
Expression of IL10R subunits within the leukocyte population (CD45(+) cells) was significantly higher in primary brain tumors than in metastases. PMID: 28982901
This study showed that lack of association with schizophrenia was detected for IL10 and IL10RA single polymorphisms and haplotypes. PMID: 27397081
The IL-10RA rs9610 A allele was increased in rheumatoid arthritis (RA) patient group compared with control subjects. Interestingly, significant differences were detected both in the allele and genotype frequencies of rs9610 between anti-CCP (anti-cyclic citrullinated peptide) positive patients and anti-CCP negative patients. The findings suggest that IL-10RA rs9610 polymorphism might contribute to RA susceptibility. PMID: 27796662
These results suggest that S138G loss-of-function polymorphism of the IL-10R1 may be important risk factor in increasing susceptibility to multiple sclerosis. PMID: 28225209
Results reveal the structure of box1 from class II cytokine receptors IFNLR1 and IL10RA bound to the FERM-SH2 domain of human JAK1, identifying a consensus motif for JAK1 interaction. PMID: 27133025
a JAK2 Inhibitor Suppresses a BCL6-dependent IL10RA/JAK2/STAT3 Pathway in High Grade B-cell Lymphoma. PMID: 27268052
genetic association study in cohort of infants/children: Data suggest perianal fistulas are exhibited in patients with very early-onset inflammatory bowel disease with IL10 receptor mutations (IL10RA/B); prognosis and response to treatment are poor. PMID: 25373860
IL-10Ralpha expression is post-transcriptionally regulated by miR-15a, miR-185, and miR-211 in melanoma PMID: 26631117
SNP3 polymorphism is associated with myocardial infarction; may generate some conformational rearrangements of the IL-10R1 receptor domain which affects IL-10 complex binding and alters its downstream signal PMID: 24566517
High IL10RA expression is associated with diffuse large B-cell lymphoma. PMID: 25733167
Inflammatory bowel disease (IBD) in infancy is phenotypically and genetically different disease entity from adult-onset or older child-onset IBD. It has a strong association with IL-10 receptor gene. PMID: 24785691
IL10R1 loss-of-function A536/S138G polymorphism may contribute to recurrent pregnancy loss pathogenesis PMID: 24689510
Very early onset inflammatory bowel disease associated with aberrant trafficking of IL-10R1 and cure by T cell replete haploidentical bone marrow transplantation. PMID: 24519095
Mutations in the IL10RA gene is associated with ulcerative colitis. PMID: 24216686
Case Report: pediatric ulcerative colitis with a novel point mutation within the IL10RA promoter (the -413G->T), inherited from his mother. PMID: 24379584
This report confirms the genetic defect of IL-10RA in neonatal-onset inflammatory bowel disease. PMID: 23839161
IFN-gamma selectively induced the expression of IL-10R1 on intestinal epithelia, predominantly on the apical membrane of polarized epithelial cells. PMID: 24367025
A mutation in TLR4 (rs4986790) and IL10RA (rs22291130) was significantly associated with Mycobacterium avium subspecies paratuberculosis-positive Crohn's disease patients. PMID: 23455702
5 patients with an IL-10R1 or IL-10R2 deficiency developed B-cell non-Hodgkin lymphoma between the ages of 5 and 6 years (which was recurrent in 1 patient). PMID: 24089328
IL10RA polymorphisms are associated with ulcerative colitis. PMID: 22550014
In the Chinese Han population, missense SNPs within the exons of the IL-10R1 gene do not contribute to the development of systemic lupus erythematosus PMID: 22652629
The results suggest that genetic polymorphisms in TNF and IL10RA genes may modify the association between blood transfusion and NHL risk. PMID: 22649007
IL-10R1 is a novel substrate of betaTrCP-containing ubiquitin E3 ligase, a novel negative regulatory mechanism that may potentially affect IL-10 function PMID: 22087322
We found an IL10R variant, which may be associated with a decreased response to the cytokine in one patient. PMID: 22155628
IL-10R1 S138G loss-of-function polymorphism is associated with extrapulmonary tuberculosis risk development in Tunisia. PMID: 21553229
The haplotype -185/-116 of IL10 receptor alpha in combination with the haplotype -754/-750 of IL10 receptor beta contributed towards mild malaria. PMID: 21814839
IL10R1-G330R does not alter surface expression but duration of STAT phosphorylation, indicating that the position of G330 is important in stabilizing the STAT signal. PMID: 21654841
linkage disequilibrium (LD) blocks were formed in IL10 and IL10RA PMID: 21532858
IL-10R1 expression on CD4+ T cells and signalling in PBMCs were down-regulated in lupus nephritis (LN) patients, indicating that IL-10 and its receptor may have a special role in LN pathogenesis PMID: 21635228
Besides overall immune health, host genetic factors influence mortality, retinitis progression, and retinal detachment in patients with AIDS and CMV retinitis that are receiving HAART. PMID: 21396623
in the Lebanese population, the loss-of-function allele IL-10R1-S138G (SNP3) is unlikely to provide a protective effect against ulcerative colitis and that both IL-10R1 variants do not correlate with inflammatory bowel disease PMID: 20186944
G carriers for the -536AG IL-10R1 gene polymorphism had higher systolic and diastolic pressures, and IL-10 levels; and obese G carriers had an increased waist-to-hip ratio. PMID: 19798061
cmvIL-10 forms a disulfide-linked homodimer that binds two sIL-10R1 molecules. PMID: 12093920
IL-10 receptor stimulated the rapid translocation of IL-10E1 to the cell nucleus and the activation of TIMP-1 expression in primary human prostate tumor cell lines. PMID: 12496489
Abnormal interleukin 10Ralpha expression contributes to the maintenance of elevated cyclooxygenase-2 in non-small cell lung cancer cells. PMID: 12591723
This investigation reveals three previously unrecognized polymorphisms of IL-10R1 (SNP3, SNP4, and SNP5), two of which result in an amino acid substitution; substitution in S138G variant may interfere with binding of IL-10 to IL-10R1. PMID: 12759436
Homozygosity of the IL-10R1 G330R allele is associated with schizophrenia and may contribute to the expression of disease phenotype in susceptible individuals. PMID: 17066477
Genetic variation in IL-10RA/IL-22 genes may play a modulatory role in the outcome of hepatitis C infection. PMID: 17845543
The IL-4R Ile50/Ile50 and IL-10R2 G520/G520 and G520/A520 genotypes were shown to determine the susceptibility to SLE (systemic lupus erythematosus)in a Chinese population PMID: 17988330
The peripheral blood neutrophils of septic patients constitutively display abundant levels of surface IL-10R1. PMID: 18308712
IL-10R is associated with the progression of the renal cell carcinoma PMID: 18344594
Data demonstrate a significant relation between cervical concentrations of IL-10 and single nucleotide polymorphisms in the IL-10 receptor alpha and beta genes. PMID: 18674658
The IL-10R1 S138G loss-of-function allele and ulcerative colitis are reported. PMID: 18800073
a variant rs17121510 in the interleukin-10 receptor antagonist (IL-10RA) gene for allele (p = 0.01) and genotype (p = 3.34x10(-4)) may have a role in preterm birth PMID: 18818748
IL-10R1 variants differentially reduce the signaling activity of cmvIL-10 PMID: 19016528
Mutations in genes encoding the IL10R subunit proteins were found in patients with early-onset enterocolitis, involving hyperinflammatory immune responses in the intestine. PMID: 19890111
The capacity of neutrophils to respond to IL-10, as assessed by Stat3 tyrosine phosphorylation, SOCS-3 expression, and modulation of cytokine production, is dependent on the level of expression of IL-10R1. PMID: 11490020

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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