Recombinant Human Interferon-alpha / IFNA Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-3002
Recombinant Human Interferon-alpha / IFNA Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-3002
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | Fc |
Host Species | Human |
Accession | NP_002160.1 |
Synonym | IFN-alpha, IFN-alpha-5, IFN-alphaG, INA5, INFA5, leIF G, RP11-380P16.5 |
Background | Interferon, alpha 5 (IFNA5) belongs to the alpha/beta interferon family. IFNA5 is the only IFNA subtype detected in normal liver, while a mixture of subtypes is observed in the liver tissue of patients with chronic hepatitis C. Interferons are produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. IFN-alpha, the first cytokine to be produced by recombinant DNA technology, has emerged as an important regulator of growth and differentiation, affecting cellular communication and signal transduction pathways as well as immunological control. Originally discovered as an antiviral substance, the efficacy of IFN-alpha in malignant, viral, immunological, angiogenic, inflammatory, and fibrotic diseases suggests a spectrum of interrelated pathophysiologies. IFN-alpha emerged as a prototypic tumor suppressor protein that represses the clinical tumorigenic phenotype in some malignancies capable of differentiation. |
Description | A DNA sequence encoding the human IFNalpha G (NP_002160.1) (Leu 22-Glu 189) was fused with the Fc region of human IgG1 at the N-terminus. |
Source | HEK293 |
Predicted N Terminal | Glu 20 |
AA Sequence | Leu 22-Glu 189 |
Molecular Weight | The recombinant human IFNalpha G/Fc chimera is a disulfide-linked homodimer. The reduced monomer comprises 405 a.a. with a predicted molecular mass of 46.3 kDa. As a result of glycosylation, rh IFNalpha G/Fc monomer migrates as an approximately 48-50 kDa band in SDS-PAGE under reducing conditions. |
Purity | >95% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus (VSV). The EC50 for this effect is 0.16-0.8 ng/mL. |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |