Recombinant Human Intelectin-1 (ITLN1) Protein (His&Myc)

Name: Recombinant Human Intelectin-1 (ITLN1) Protein (His&Myc)
Brand: Beta LifeScience
SKU: BLC-00638P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Intelectin-1 (ITLN1) Protein (His&Myc) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	Q8WWA0
Target Symbol	ITLN1
Synonyms	(ITLN-1)(Endothelial lectin HL-1)(Galactofuranose-binding lectin)(Intestinal lactoferrin receptor)(Omentin)
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-10His&C-Myc
Target Protein Sequence	TDEANTYFKEWTCSSSPSLPRSCKEIKDECPSAFDGLYFLRTENGVIYQTFCDMTSGGGGWTLVASVHENDMRGKCTVGDRWSSQQGSKAVYPEGDGNWANYNTFGSAEAATSDDYKNPGYYDIQAKDLGIWHVPNKSPMQHWRNSSLLRYRTDTGFLQTLGHNLFGIYQKYPVKYGEGKCWTDNGPVIPVVYDFGDAQKTASYYSPYGQREFTAGFVQFRVFNNERAANALCAGMRVTGCNTEHHCIGGGGYFPEASPQQCGDFSGFDWSGYGTHVGYS
Expression Range	19-298aa
Protein Length	Full Length of Mature Protein
Mol. Weight	38.6 kDa
Research Area	Cardiovascular
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Lectin that specifically recognizes microbial carbohydrate chains in a calcium-dependent manner. Binds to microbial glycans that contain a terminal acyclic 1,2-diol moiety, including beta-linked D-galactofuranose (beta-Galf), D-phosphoglycerol-modified glycans, D-glycero-D-talo-oct-2-ulosonic acid (KO) and 3-deoxy-D-manno-oct-2-ulosonic acid (KDO). Binds to glycans from Gram-positive and Gram-negative bacteria, including K.pneumoniae, S.pneumoniae, Y.pestis, P.mirabilis and P.vulgaris. Does not bind human glycans. Probably plays a role in the defense system against microorganisms (Probable). May function as adipokine that has no effect on basal glucose uptake but enhances insulin-stimulated glucose uptake in adipocytes. Increases AKT phosphorylation in the absence and presence of insulin. May interact with lactoferrin/LTF and increase its uptake, and may thereby play a role in iron absorption.
Subcellular Location	Cell membrane; Lipid-anchor, GPI-anchor. Secreted.
Database References	HGNC: 18259 OMIM: 609873 KEGG: hsa:55600 STRING: 9606.ENSP00000323587 UniGene: Hs.50813
Tissue Specificity	Highly expressed in omental adipose tissue where it is found in stromal vascular cells but not in fat cells but is barely detectable in subcutaneous adipose tissue (at protein level). Highly expressed in the small intestine. Also found in the heart, testi

Gene Functions References

chemerin and leptin are elevated and omentin-1 and visfatin levels are decreased in Gestational diabetes mellitus women complicated by obesity. PMID: 29337272
Study shows omentin Val/Val (mutant homozygote) genotype increases predisposition to acne vulgaris by probably disrupting overall protein function of omentin. PMID: 30301508
The study showed for the first time serum omentin and visfatin levels to be decreased after lung transplantation in end-stage lung disease patients PMID: 29277835
ITLN1, PPARg AND TNFa GENE EXPRESSION IN VISCERAL ADIPOSE TISSUE. PMID: 30188100
Omentin may play a role in the pathophysiology of preterm births but it is not involved in the mechanism of preterm premature rupture of the membranes. PMID: 28440092
Loss of Omentin-1 expression is Associated with Carotid Plaque Instability among Ischemic Stroke Patients. PMID: 29225325
Val109Asp polymorphic marker of intelectin 1 gene is associated with abdominal obesity. PMID: 29482534
Reduced serum omentin-1 is associated with inflammatory mechanism and insulin resistance in the pathogenesis of diabetic nephropathy in type 2 diabetes mellitus patients. PMID: 28864059
The aims of this study were to evaluate and correlate circulating chemerin, apelin, vaspin, and omentin-1 levels in obese type 2 diabetic Egyptian patients with coronary artery stenosis (CAS), and to assess their usefulness as noninvasive diagnostic biomarkers. PMID: 28957639
six-month lifestyle weight loss intervention significantly increased serum levels in obese children PMID: 28833541
ITLN1 expression was significantly increased in asthmatic airways and in lesional skin of atopic dermatitis patients PMID: 28224996
Plasma omentin-1 level was significantly higher in patients with good coronary collateral circulation (CCC) than those with poor CCC (566.57+/-26.90 vs. 492.38+/-19.70 ng/mL, p=0.024). Besides, omentin-1 was positively correlated with total cholesterol, high-density lipoprotein, and gensini score but inversely with hyperlipidemia and body mass index (all p values0.05). PMID: 28123148
In conclusion, our study, which focused on hepatic FGF21 and omentin-1 mRNA expression, confirmed marked expression of both molecules in the liver of morbidly obese patients with NAFLD. PMID: 28820393
Myocardial injury leads to a decrease in ITLN1 expression in the heart and a corresponding increase in plasma levels PMID: 28687077
the effects of omentin-1 on KLF2 expression are mediated by p53. PMID: 29408455
Omentin levels are increased in cirrhotic patients without portal vein thrombosis, but do not influence platelet activity. PMID: 28465646
Omentin-1 levels are closely associated with the endogenous insulin reserve and may be used in follow-up of patients. PMID: 28073124
Omentin expression increases in the epicardial adipose tissueof non-obese coronary artery disease patients, despite a decrease in plasma levels, suggesting that omentin may play a role in the pathogenesis of coronary artery disease PMID: 27450783
Omentin-1 concentrations may be related to increased stroke risk PMID: 27298015
A decreased level of circulating omentin negatively correlated with white blood cells and procalcitonin in patients with acute respiratory distress syndrome. PMID: 27607575
Serum levels of omentin-1 were positively associated with increases in glycemia and incident type 2 diabetes in an older population. PMID: 28679518
These findings suggested that elevated serum omentin levels were only very mildly related to the changes in cardiac volume and function in chronic heart failure patients PMID: 27270246
Data suggest that circulating omentin-1 levels are lower in patients with PCOS (polycystic ovary syndrome) as compared to control subjects; circulating omentin-1 levels appear to correlate with insulin resistance but do not appear to correlate with overweight/obesity in these patients. [META-ANALYSIS] PMID: 27908216
In conclusion, DAB2 and Intelectin-1 are newly identified positive markers of mesothelioma and have potential to be included in future immunohistochemical marker panels for differentiation of epithelioid mesothelioma from pulmonary adenocarcinoma PMID: 28394802
This study showed that consumption of olive oil-rich diet tended to increase omentin and adiponectin in comparison with the usual diet. PMID: 27931137
Omentin concentrations were inversely associated with MMP-3 levels in rheumatoid arthritis patients. This relationship was influenced by population origin, rheumatoid arthritis activity and erythrocyte sedimentation rate. PMID: 27476070
High omentin expression is associated with Colorectal Cancer. PMID: 27216184
Our results indicate that the Asp allele of Val109Asp (T allele of rs2274907) is more frequent among men with coronary artery disease than healthy men, so it is possibly a risk factor for coronary artery disease in men only. PMID: 28325076
In type 2 diabetes mellitus, both chemerin and omentin enhance the body sensitivity to insulin, which results in increased glucose uptake and increased expression of the omentin gene was reported in nasal polyps and mesothelioma. PMID: 27516571
High omentin expression is associated with osteoporosis. PMID: 27614458
There was no difference in serum chemerin, vaspin and omentin-1 between polycystic ovary syndrome patients and healthy controls. PMID: 27225862
Atorvastatin increased serum omentin-1 concentrations in patients with coronary artery disease in a dose-dependent manner. PMID: 27749321
Omentin-1 might exert a negative effect on bone remodelling in girls with anorexia nervosa by inhibiting both bone formation and resorption. The OPG/sRANKL system plays an important role in the latter. PMID: 26662650
Low serum Omentin-1 level is Associated with Dilated Cardiomyopathy. PMID: 27313334
omentin, as compared to vaspin, seems to provide a better predictor of insulin resistance in obese individuals. PMID: 27449535
Data suggest that nonlinear resistance training or aerobic interval training for 12 weeks produces no significant changes in serum levels of omentin-1 and interleukin-18 in obese men; however, during a 4-week detraining period, omentin-1 decreases significantly and IL-18 increases significantly. PMID: 27636349
Omentin-1 expression in patients with coronary artery disease was lower in epicardial adipose tissue adjacent to coronary stenotic segments than non-stenotic segments PMID: 27352781
omentin reduces the development of atherosclerosis by reducing inflammatory response of macrophages through the Akt-dependent mechanisms PMID: 26714927
Omentin-1 levels were markedly reduced in coronary endothelium and epicardial fat but increased in plasma and atheromatous plaques (macrophages/SMCs) in coronary artery disease (CAD) patients compared with non-CAD patients PMID: 26790473
The mean serum omentin-1 concentration in adolescent girls with anorexia nervosa was higher than that of controls and obese girls. PMID: 25804090
Omentin levels were lower in both obese and non-obese PCOS patients compared to healthy controls. PMID: 26291814
Data show that the circulating omentin-1 levels were dramatically decreased in renal cell carcinoma (RCC) patients. PMID: 26539805
Although no significant difference was detected regarding omentin Val109Asp polymorphism, Val/Val genotype frequency was found to be more in patient group than control group. It may be speculated that Val/Val genotype increases the tendency for CAD. PMID: 24370683
Plasma omentin-1 concentrations were decreased in non-obese polycystic ovary syndrome individuals PMID: 26514948
Data suggest serum omentin/ITLN1 levels are down-regulated in elderly population (ages 61-82 years) with type 2 diabetes and polyneuropathy, independently of established risk factors of polyneuropathy; ITLN1 is positively correlated with adiponectin. PMID: 26094489
Serum omentin was associated with cardiac autonomic neuropathy in type 2 diabetes patients. PMID: 26466574
analysis of expression of maternal omentin-1 in humans and in the rat animal model PMID: 26144294
Lower levels of serum omentin in patients with pseudoexfoliation syndrome compared with healthy subjects may support the theory of systemic nature of the disease. PMID: 25264991
A decrease in omentin-1 levels could be an independent mortality risk factor in patient with diabetes on hemodialysis. PMID: 26293449
This study clearly indicates that acute aerobic exercise elicits a pro-inflammatory response (e.g. CHI3L1) with a lower anti-inflammatory effect (e.g. ITLN-1) in obese individuals. PMID: 26316585

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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