Recombinant Human Integrin Alpha-L (ITGAL) Protein (His)

Name: Recombinant Human Integrin Alpha-L (ITGAL) Protein (His)
Brand: Beta LifeScience
SKU: BLC-00167P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Integrin Alpha-L (ITGAL) Protein (His) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 90% as determined by SDS-PAGE.
Activity	Not tested.
Uniprotkb	P20701
Target Symbol	ITGAL
Synonyms	(CD11 antigen-like family member A)(Leukocyte adhesion glycoprotein LFA-1 alpha chain)(LFA-1A)(Leukocyte function-associated molecule 1 alpha chain)(CD antigen CD11a)
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	C-6His
Target Protein Sequence	CIKGNVDLVFLFDGSMSLQPDEFQKILDFMKDVMKKLSNTSYQFAAVQFSTSYKTEFDFSDYVKRKDPDALLKHVKHMLLLTNTFGAINYVATEVFREELGARPDATKVLIIITDGEATDSGNIDAAKDIIRYIIGIGKHFQTKESQETLHKFASKPASEFVKILDTFEKLKDLFTELQKKIYVIEG
Expression Range	150-336aa
Protein Length	Partial
Mol. Weight	28.2 kDa
Research Area	Immunology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrin ITGAL/ITGB2 is a receptor for F11R. Integin ITGAL/ITGB2 is a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL. Involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. Contributes to natural killer cell cytotoxicity. Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils. Required for generation of common lymphoid progenitor cells in bone marrow, indicating a role in lymphopoiesis. Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages.
Subcellular Location	Cell membrane; Single-pass type I membrane protein.
Protein Families	Integrin alpha chain family
Database References	HGNC: 6148 OMIM: 153370 KEGG: hsa:3683 STRING: 9606.ENSP00000349252 UniGene: Hs.174103
Tissue Specificity	Leukocytes.

Gene Functions References

LFA-1 cross-linking recruits and activates FAK1 and PYK2 to phosphorylate LAT selectively on a single Y-171 site that binds to the GRB2-SKAP1 complex and limits dwell times of T-cells with dendritic cells PMID: 28699640
Data show that integrin alphaLbeta2 (LFA-1) is oriented relative to actin retrograde flow. PMID: 29229906
These results demonstrated that histone demethylase JMJD3 regulates CD11a expression in lupus T cells by affecting the H3K27me3 levels in the ITGAL (CD11a) promoter region, and JMJD3 might thereby serve as a potential therapeutic target for SLE. PMID: 28430662
Data show a nadir of CD11a expression at 2 years of natalizumab treatment in multiple sclerosis, at the peak of incidence of progressive multifocal leukoencephalopathy, indirectly suggesting that a lack of these molecules may play a role in the onset of PML in NTZ-treated patients. PMID: 24258149
Extracellular ISG15 signals cytokine secretion through the LFA-1 integrin receptor (CD11a/CD18) in natural killer cells. PMID: 29100055
Suggest possible role for lymphocyte CD11a in the pathogenesis immune thrombocytopenia. PMID: 26884833
The alphaI domain, tethered through its N-linker and a disulfide to a stable beta-ribbon pillar near the center of the platform, can undergo pivoting and tilting motions that appear buffered by N-glycan decorations that differ between alphaL and alphaX subunits. PMID: 26936951
beta2 integrins-mediated chemosensory adhesion and migration of polymorphonuclear leukocytes on the vascular graft surface, bacterial cellulose. PMID: 25231265
Data show that interleukin-18 was involved in the eosinophil-mediated death of colon carcinoma cells via upregulation of CD11a/CD18 and ICAM-1 adhesion molecules. PMID: 26216891
shear-induced CD11/CD18 cleavage is phenotypic of neutrophilic cells, including those derived from HL60 cells PMID: 24276331
Data show that leucocyte function-associated antigen-1 (integrin alphaLbeta2), DNAX accessory molecule-1 (DNAM-1) or tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) enhance osteoclast survival when co-cultured with activated NK cells. PMID: 25684021
LFA-1 function is regulated through conformational changes that include the modulation of ligand binding affinity and molecular extension. PMID: 25378583
the blocking of LFA-1/ICAM-1 interaction resulted in a significant increase in the percentage of IL-17(+) T cells expanded in nonclassical monocyte/T-cell cocultures PMID: 25678252
Studied signaling mechanisms of Lymphocyte function-associated antigen-1 (LFA-1) using NMR analysis of the dynamics of the LFA-1 I-domain. PMID: 25147050
CD11a, CD11c, and CD18 gene polymorphisms and susceptibility to Behcet's disease in Koreans. PMID: 25155097
ITGAL is overexpressed in systemic lupus erythematosus CD4+ T cells, but expression is not linked to the typical clinical and serological parameters associated with the disease. PMID: 24238281
there was reduced expression of CD11a, CD11b, and CD18 in trisomy 12 cases with NOTCH1 mutations compared with wild type. PMID: 24829201
We conclude, that deficiency of CD11a expression should be added to the diagnostic criteria of AML-M7, classical DS-AML and TMD. PMID: 23450818
genetic polymorphism is associated with atopic disease and relevant allergic indices PMID: 23726040
Distribution of integrin beta(2) is also markedly altered in M. tuberculosis-infected DCs. A corresponding reduction in the alphaL (CD11a) and alphaM (CD11b) subunits that associate with integrin beta(2) was also observed. PMID: 23981064
Allele frequencies were determined in the blood donor population as follows: 0.318 for HNA-1a, 0.668 for HNA-1b, 0.014 for HNA-1c, 0.768 for HNA-3a, 0.232 for HNA-3b, 0.882 for HNA-4a, 0.118 for HNA-4b, 0.736 for HNA-5a and 0.264 for HNA-5b. PMID: 23398146
The results of this study suggest that polyamine-induced suppression of LFA-1 expression occurs via enhanced methylation of ITGAL. PMID: 23418509
Binding kinetics differences between LFA-1 and macrophage-1 antigen (Mac-1) are quantified and insight provided into their distinct functions in the neutrophil inflammatory cascade. PMID: 23514737
common and rare variants in FTO, SERPINA12, and ITGAL predict PON1 acti PMID: 23160181
Flow-based adhesion assay showed that engagement of CD103 or LFA-1, together with TCR, enhances the strength of the T-cell/target cell interaction PMID: 23188505
Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A PMID: 22384114
Binding of LtxA to LFA-1 resulted in the internalization of both LtxA and LFA-1, with LtxA localizing specifically to the lysosomal compartment. PMID: 22467872
This work demonstrates an intricate coupling between conformation and lateral diffusion of LFA-1 and further underscores the crucial role of mobility for the onset of LFA-1 mediated leukocyte adhesion. PMID: 22411821
DNA hypermethylation at the CD11a locus contributes to the lowered expression of CD11a in biliary atresia CD4+ T cells. PMID: 22206678
CD70 and CD11a facilitate the survival of T and B lymphocytes and indirectly enhance the destruction of platelets in immune thrombocytopenia. PMID: 21541792
Regulator of G protein signaling (RGS) represses Galpha-dependent signaling during chemokine-activated integrin alphaLbeta2- and alpha4beta1- dependent T cell adhesion. PMID: 21705617
N-terminal myr-tagged SKAP1 for membrane binding facilitated constitutive RapL membrane and Rap1 binding and effectively substituted for PI3K and TCR ligation in the activation of LFA-1 in T cells. PMID: 21669874
hyperketonemia can induce monocyte adhesion to endothelial cells and it is mediated via increased ICAM-1 expression in endothelial cells and increased expression and affinity of LFA-1 in monocytes PMID: 21540444
We show that sequential cleavage of C-terminal amino acids from the beta(2) cytoplasmic tail of LFA-1, by cathepsin X, enhances binding of the adaptor protein talin to LFA-1 and triggers formation of the latter's high-affinity form PMID: 21454358
Bordetella pertussis adenylate cyclase toxin binds to T cells via LFA-1 and induces its disengagement from the immune synapse PMID: 21576384
Studies indicate that transformation of LFA1 from a bent, resting form to an extended conformation is essential for T cell migration and the formation of an immunological synapse. PMID: 21597477
LFA-1-induced stabilization of ARE-containing mRNAs in T cells is dependent on HuR, and occurs through the Vav-1, Rac1/2, MKK3 and p38MAPK signaling cascade PMID: 21206905
RFX1 recruits SUV39H1 to the promoter regions of the CD11a and CD70 genes in CD4(+) T cells, thereby regulating local H3K9 tri-methylation levels. PMID: 21192791
2B4-mediated increase in natural killer (NK) cell adhesion to tumor targets is actin- and LFA-1-dependent, demonstrating that inside-out signaling is very fast and its effects can be detected as early as 90 seconds after cell contact. PMID: 21270395
Findings support the idea that calpain is involved in the turnover of LFA-1 adhesions at the rear of the cell. PMID: 21152086
the high-affinity state of LFA-1 on T cells is critical to induce targeted movements of both ICAM-1 and MHC class II to the immunological synapse on APCs. PMID: 20681010
Decreased chemotactic response and downregulated expression of CD11a in an experimental model of depression could be an important mechanisms to induce impaired immune responses in experimental and clinical depression. PMID: 20516718
Case Report: Possible acceleration of regression by antibiotic treatment in benign cephalic histiocytosis with infiltration of CD11a/CD11c+ macrophages. PMID: 20574620
Human neutrophil rolling on E- or P-selectin induces the extended alpha(L)beta(2) integrin conformation through signaling triggered by PSGL-1 engagement. PMID: 20445017
T helper (Th)17 cells adhere to mesenchymal stem cells through the joined involvement of CCR6 and CD11a/CD18 and their respective ligands, interactions that induce a regulatory phenotype in fully polarized tissue-infiltrating Th17 cells. PMID: 20511548
These results provide an example of the stabilizing effect of polar interactions within the low dielectric environment of the membrane interior and demonstrate its importance in the regulation of alpha L beta 2 function. PMID: 20338181
Activation-induced conformational changes in the I domain region of lymphocyte function-associated antigen 1 PMID: 11792712
important role for the integrin LFA-1 and the microtubular cytoskeleton in the Ca(2)(+)-mediated activation of PYK2 in T-lymphocytes PMID: 11867690
regulation of LFA-1 activation and neutrophil arrest on intercellular adhesion molecule 1 in shear flow PMID: 11929876
cd11a may play an important role in pathogenic process(in COPD) PMID: 11953106

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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