Recombinant Human Insulin-Like Growth Factor 2 Mrna-Binding Protein 3 (IGF2BP3) Protein (His)

Name: Recombinant Human Insulin-Like Growth Factor 2 Mrna-Binding Protein 3 (IGF2BP3) Protein (His)
Brand: Beta LifeScience
SKU: BLC-06701P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Insulin-Like Growth Factor 2 Mrna-Binding Protein 3 (IGF2BP3) Protein (His) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	O00425
Target Symbol	IGF2BP3
Synonyms	(IGF2 mRNA-binding protein 3)(IMP-3)(IGF-II mRNA-binding protein 3)(KH domain-containing protein overexpressed in cancer)(hKOC)(VICKZ family member 3)
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	MNKLYIGNLSENAAPSDLESIFKDAKIPVSGPFLVKTGYAFVDCPDESWALKAIEALSGKIELHGKPIEVEHSVPKRQRIRKLQIRNIPPHLQWEVLDSLLVQYGVVESCEQVNTDSETAVVNVTYSSKDQARQALDKLNGFQLENFTLKVAYIPDEMAAQQNPLQQPRGRRGLGQRGSSRQGSPGSVSKQKPCDLPLRLLVPTQFVGAIIGKEGATIRNITKQTQSKIDVHRKENAGAAEKSITILSTPEGTSAACKSILEIMHKEAQDIKFTEEIPLKILAHNNFVGRLIGKEGRNLKKIEQDTDTKITISPLQELTLYNPERTITVKGNVETCAKAEEEIMKKIRESYENDIASMNLQAHLIPGLNLNALGLFPPTSGMPPPTSGPPSAMTPPYPQFEQSETETVHLFIPALSVGAIIGKQGQHIKQLSRFAGASIKIAPAEAPDAKVRMVIITGPPEAQFKAQGRIYGKIKEENFVSPKEEVKLEAHIRVPSFAAGRVIGKGGKTVNELQNLSSAEVVVPRDQTPDENDQVVVKITGHFYACQVAQRKIQEILTQVKQHQQQKALQSGPPQSRRK
Expression Range	1-579aa
Protein Length	Full Length
Mol. Weight	69.7 kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	RNA-binding factor that may recruit target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. Binds to the 3'-UTR of CD44 mRNA and stabilizes it, hence promotes cell adhesion and invadopodia formation in cancer cells. Binds to beta-actin/ACTB and MYC transcripts. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs.
Subcellular Location	Nucleus. Cytoplasm. Note=Found in lamellipodia of the leading edge, in the perinuclear region, and beneath the plasma membrane. The subcytoplasmic localization is cell specific and regulated by cell contact and growth. Localized at the connecting piece and the tail of the spermatozoa. Colocalized with CD44 mRNA in RNP granules. In response to cellular stress, such as oxidative stress, recruited to stress granules.
Protein Families	RRM IMP/VICKZ family
Database References	HGNC: 28868 OMIM: 608259 KEGG: hsa:10643 STRING: 9606.ENSP00000258729 UniGene: Hs.700696
Tissue Specificity	Expressed in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal follicles of ovary and gonocytes of testis, growing oocytes, spermatogonia and semen (at protein level). Expressed in cervix adenocarcinoma, in testicular, pancreatic

Gene Functions References

Data show that insulin-like growth factor-II mRNA-binding protein 2 and 3 (IMP2/3)-miR-200a-progesterone receptor axis represents a double-negative feedback loop and serves as a new potential therapeutic target for the treatment of Triple-negative breast cancer (TNBC). PMID: 29217458
THADA fusion is a mechanism of IGF2BP3 activation and IGF1R signaling in thyroid cancer. PMID: 28193878
High expression of IMP3 in adult mature testicular teratomas supports their malignant nature. PMID: 24605891
tubal secretory cells with IMP3 signatures showing growth advantage could potentially serve as a latent precancer biomarker for tubal or pelvic serous carcinomas in women. PMID: 25014991
These results suggested that pancreatic carcinogenesis involves an increased IMP3 mRNA expression, and it may become valuable diagnostic and prognostic markers as well as potential therapeutic targets for pancreatic cancer. PMID: 25183049
the combination of p16/IMP3 expression was found to improve the discrepant results between cervical cytologic and histologic diagnoses PMID: 23923073
High IMP3 expression is associated with hepatocellular carcinoma. PMID: 24647926
Our results strengthen the association of IMP3 with the regulation of epithelial to mesenchymal transition in breast cancers PMID: 25031719
Post-transcriptional regulation of cyclins D1, D3 and G1 and proliferation of human cancer cells depend on IMP-3 nuclear localization. PMID: 23812426
IMP3 upregulates HMGA2 mRNA by opposing its interaction with Ago2/let-7. PMID: 24703842
The results of this study indicate that IMP3 was overexpressed in sacral chordoma and this expression was associated with tumor invasion and recurrence. PMID: 24135846
IMP3 may be involved in the process and progression of high-grade serous carcinoma of the fallopian tube. PMID: 25038792
Absence of IMP3 expression is associated with a significantly reduced risk of lymphovascular invasion in pT1 esophageal adenocarcinoma PMID: 24726263
Diffuse IMP3 expression (>25%) in MAC from patients with gastric adenocarcinoma was associated with shorter survival. PMID: 24457229
This review focuses on the studies of IMP3 expression in different cancers and emphasizes the potential utility of IMP3 in routine surgical pathology practice. PMID: 24713990
IMP3 expression was positive in esophageal adenocarcinoma and Barrett's esophagus with high-grade dysplasia. Barrett's esophagus and esophageal squamous epithelium had no IMP3 expression PMID: 25141312
High IMP3 was associated with metastasis in prostate cancer. PMID: 24615121
High-grade dysplasia and gastric carcinomas can be distinguished from low-grade dysplasia and inflammatory lesions of the gastric mucosa with a high specificity and good sensitivity using a combination of the immunohistochemical markers IMP3 and p53 PMID: 24966917
IMP-3 expression is not a useful discriminator between dysplastic nevi and melanoma nor a good prognostic marker in melanoma. PMID: 26403409
The specificity for IMP3, GLUT-1, and EMA for differentiating malignant mesotheliomas and benign mesothelial proliferations was 100% PMID: 25376377
expression in preoperative biopsy material predictive of perineural invasion in patients with oral squamous cell carcinoma PMID: 27863864
IMP3 appears to play an important role in tumor invasion in patients with lung adenocarcinoma and may serve as a useful prognostic biomarker in these patients. PMID: 26608347
IMP3 represents a biomarker of unfavorable prognosis for clear cell carcinomas of the ovary, even in stage I patients. PMID: 26837693
results suggest that IMP3 facilitates pancreatic ductal adenocarcinoma progression by enhancing the pro-metastatic behaviour of tumour cells PMID: 25886367
EGF stimulates IMP-3 expression. PMID: 26386725
MP3 may play a certain role in hydatidiform moles progression, and it may serve as a prognostic biomarker for this disease. PMID: 27238915
Testing for a combination of elevated IMP3 and Lin28B levels could further facilitate the identification of a patient subgroup with the worst ovarian cancer prognosis. PMID: 26158423
we show that SOX2 is a transcriptional target of SLUG. These data establish a novel mechanism of breast tumor initiation involving IMP3 and they provide a rationale for its association with aggressive disease and poor outcome. PMID: 25982283
This is the first study evaluating the IMP3 immunohistochemical expression in Phyllodes tumor. Its expression correlates with histological grade and could be used in the differential diagnosis of fibroepithelial tumors and in prognostication. PMID: 26353850
IMP3 is a reliable marker for the diagnosis of colorectal cancer in endoscopic biopsies PMID: 28412210
Positive expression of IMP3 in spinal giant cell tumor was associated with increased microvessel density and expression of IGF2. PMID: 25740666
Tissue overexpression of IMP3 might be used as a predictor of advanced disease or lymph node metastasis, and is associated with poorer prognosis in gastric cancers. PMID: 28011483
IMP-3 has a role in promoting migration and invasion of melanoma cells by modulating the expression of HMGA2 and predicts poor prognosis in melanoma PMID: 25380351
The role of IMP3 as a marker of poor outcome in gliomas PMID: 25695077
IMP3 directly interacts with ULBP2 mRNA, leading to ULBP2 transcript destabilization and reduced ULBP2 surface expression and indirectly downregulates MICB with a mechanism functionally distinct from that of ULBP2. PMID: 26982091
This study demonstrated that Genes overexpressed in Pilomyxoid Astrocytoma vs. Pilocytic Astrocytoma, ranked according to fold-change, included developmental genes H19, DACT2,COL2A1; COL1A1 and IMP3. PMID: 25521223
Describe chromosome 12p abnormalities and IMP3 expression in prepubertal pure testicular teratomas. PMID: 26826410
This study aimed to demonstrate the usefulness of mRNA expression levels of IMP3 in brush cytology specimens combined with cytology for the diagnosis of malignant biliary stricture PMID: 27399126
IMP-3 and its protein partners ILF3/NF90 and PTBP1 bind to the 3'UTRs of the cyclin mRNAs and protect them from the translational repression induced by miRNA-dependent recruitment of AGO2/GW182 complex in human cancer cells. PMID: 27840950
IMP3 promotes renal cell carcinoma cell migration and invasion by activation of NF-small ka, CyrillicB pathway. PMID: 25919292
Overexpression of IMP3 and EGFR were significantly associated with shorter periods of metastasis-free and disease-free survival of malignant phyllodes tumor of the breast. PMID: 27137988
S100P achieved 96.4% sensitivity, 93.3% specificity, 98.2% PPV, 87.5% NPV and 95.8% total accuracy, while IMP3 achieved 91.2% sensitivity, 86.7% specificity, 96.2% PPV, 72.2% NPV and 90.3% total accuracy for pancreatic ductal adenocarcinoma (PDA).Both markers were sensitive and specific for diagnosis of PDA. PMID: 27839709
CK5/6, IMP3 and TTF1 immunostaining appears to be useful to improve the accuracy of cytological diagnoses between reactive mesothelial cells, metastatic adenocarcinoma of lung and non-lung origin in pleural effusion PMID: 25337222
Our study clearly demonstrated differentiation-dependent expression of IMP3 in chondrosarcoma, and suggests IMP3 as a novel marker for differentiating problematic cases of enchondroma from well-differentiated chondrosarcomas PMID: 26948096
It was concluded that IMP3 and nucleophosmin cannot be a routine diagnostic marker for discrimination of papillary carcinomas and benign lesions. IMP3 positive staining was scarce in IMP3 positive papillary carcinomas although specifity of IMP3 is 100% PMID: 25389240
MP3 expression in biopsy specimens could be used to predict lymph node metastasis and TNM stage in colorectal cancer patients PMID: 26617820
IGF2BP3 influences a malignancy-associated RNA regulon by modulating miRNA-mRNA interactions. PMID: 27210763
IGF2BP3 as a human ontogenic master switch that restricts megakaryocyte development by modulating a lineage-specific P-TEFb activation mechanism. PMID: 28481226
Results suggest that IGF2BP3 promotes eIF4E-mediated translational activation through the reduction of EIF4E-BP2 via mRNA degradation, leading to enhanced cell proliferation. PMID: 26522719
We compressively revealed the oncogenic role of IGF2BP3 in gastric tumorigenesis and confirmed its activation is partly due to the silence of miR-34a. Our findings identified useful prognostic biomarker and provided clinical translational potential. PMID: 28399871

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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