Recombinant Human Immunodeficiency Virus Type 1 Group M Subtype K Protein Vpr (VPR) Protein (His&Myc)
Beta LifeScience
SKU/CAT #: BLC-05276P

Greater than 85% as determined by SDS-PAGE.
Recombinant Human Immunodeficiency Virus Type 1 Group M Subtype K Protein Vpr (VPR) Protein (His&Myc)
Beta LifeScience
SKU/CAT #: BLC-05276P
Collections: High-quality recombinant proteins, Other recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Description | Recombinant Human Immunodeficiency Virus Type 1 Group M Subtype K Protein Vpr (VPR) Protein (His&Myc) is produced by our E.coli expression system. This is a full length protein. |
Purity | Greater than 85% as determined by SDS-PAGE. |
Uniprotkb | P0C1P2 |
Target Symbol | VPR |
Synonyms | vprProtein Vpr; R ORF protein; Viral protein R |
Species | Human immunodeficiency virus type 1 group M subtype K (isolate 96CM-MP535) (HIV-1) |
Expression System | E.coli |
Tag | N-10His&C-Myc |
Target Protein Sequence | MEQAPEDQGPQREPNNEWTLEILEELKREAVRHFPRPWLHNLGQHIYTTYGDTWEGLEAIIRILQQLLFIHFRIGCHHSRIGIIPQRRGRNGSSRS |
Expression Range | 1-96aa |
Protein Length | Full Length |
Mol. Weight | 18.8 kDa |
Research Area | Immunology |
Form | Liquid or Lyophilized powder |
Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Storage | 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Target Details
Target Function | During virus replication, may deplete host UNG protein, and incude G2-M cell cycle arrest. Acts by targeting specific host proteins for degradation by the 26S proteasome, through association with the cellular CUL4A-DDB1 E3 ligase complex by direct interaction with host VPRPB/DCAF-1. Cell cycle arrest reportedly occurs within hours of infection and is not blocked by antiviral agents, suggesting that it is initiated by the VPR carried into the virion. Additionally, VPR induces apoptosis in a cell cycle dependent manner suggesting that these two effects are mechanistically linked. Detected in the serum and cerebrospinal fluid of AIDS patient, VPR may also induce cell death to bystander cells.; During virus entry, plays a role in the transport of the viral pre-integration (PIC) complex to the host nucleus. This function is crucial for viral infection of non-dividing macrophages. May act directly at the nuclear pore complex, by binding nucleoporins phenylalanine-glycine (FG)-repeat regions. |
Subcellular Location | Virion. Host nucleus. Host extracellular space. |
Protein Families | HIV-1 VPR protein family |