Recombinant Human IL-7RA Protein (C-Fc-6His)

Name: Recombinant Human IL-7RA Protein (C-Fc-6His)
Brand: Beta LifeScience
SKU: BL-0896NP
Availability: InStock

BL-0896NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

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Product Overview

Description	Recombinant human Interleukin-7 Receptor Subunit Alpha is produced by our Mammalian expression system and the target gene encoding Glu21-Gly236 is expressed with a human IgG1 Fc, 6His tag at the C-terminus.
Accession	P16871
Synonym	Interleukin-7 receptor subunit alpha;IL7R;IL-7R-alpha;CD127
Gene Background	Interleukin 7 Receptor alpha (IL-7Rα), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival. IL-7Rα is majorly expressed on T cells and their precursors, and early in B cell development as well, prior to the appearance of surface IgM. Dynamic regulation of IL-7Rα is important for the generation of appropriate immune responses.
Molecular Mass	52.8 KDa
Apmol Mass	75 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).
Subcellular Location	[Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 3]: Cell membrane; Single-pass type I membrane protein.; [Isoform 4]: Secreted.
Protein Families	Type I cytokine receptor family, Type 4 subfamily
Database References	HGNC: 6024 OMIM: 146661 KEGG: hsa:3575 STRING: 9606.ENSP00000306157 UniGene: PMID: 30069682 Increased signal transduction and proliferation in response to IL-7 was found in 'TT' compared to 'CC' IL7RA homozygous HIV-infected individuals providing a mechanistic explanation of the effect of rs6897932 T-allele on CD4+ T cell recovery in HIV infection. PMID: 28181541 EZH2 mutations coexisted with mutations of NOTCH1, IL7R, and PHF6 in the two Adult T-cell Acute Lymphoblastic Leukemia patients, and they responded poorly to chemotherapy and experienced difficult clinical histories and inferior outcomes PMID: 28747286 apicidin induced the acetylation of Forkhead box-containing protein, O subfamily 1, which acts as a repressor at the IL7R promoter, accompanied with depleted active histone modifications based on chromatin immunoprecipitation assay. Taken together, these results demonstrated that targeting oncogenic IL7R in ESCC by HDAC inhibitors may be a valuable therapeutic approach. PMID: 29749437 In all, IL7RA locus polymorphisms could play an important role in the predisposition to multiple sclerosis, which could contribute to a better understanding the pathogenesis of multiple sclerosis. PMID: 28446795 The results confirm the association between IL7RA exon 6 sequence polymorphisms and increased susceptibility to multiple sclerosis. PMID: 28582853 The IL7RA rs6897932 polymorphism seems to be related to increased risk of liver fibrosis progression in HCV-infected patients. Thus, the rs6897932 polymorphism could be related to the physiopathology of chronic hepatitis C(CHC) and might be used to successfully stratify the risk of CHC progression. PMID: 29742149 indicate that IL7RhighSH2B3low expression distinguishes a novel subset of high-risk B-acute lymphoblastic leukemia associated with Ikaros dysfunction PMID: 27322554 CD127 is a useful surface marker to isolate donor-antigen-specific-Tregs in operational tolerance after liver transplantation PMID: 27105585 Data demonstrate that IL-7R expression is regulated by HBX via NF-kappaB and Notch1 pathways to facilitate the activation of intracellular pathways and expression of associated molecules, and contribute to proliferation and migration of hepatoma cells. PMID: 27821177 We show that heterozygous IL7R exon deletions are common in T-B+NK+ SCID and are detectable by WES. They should be considered if Sanger sequencing fails to detect homozygous or compound heterozygous IL7R SNVs or INDELs. PMID: 27807805 The results of this study showed that IL-17 receptor (IL-17R) were clearly up regulated in mesial temporal lobe epilepsy at both mRNA and protein levels, compared with control. PMID: 27609289 the coding regions of 17 genes involved in the regulation of the immune response were determined by massive parallel sequencing. The analysis revealed 39 nonsynonymous SNPs that lead to amino acid substitutions, including the following informative genetic markers: PTPN22 c.1858C>T (rs2476601), TLR4 c.896A>G (rs4986790) and TLR4 c.1196C>T (rs4986791), IL7R c.197T>C (rs1494555) and IL7R c.412G>A (rs1494558). PMID: 28537236 The interleukin-7 receptor alpha (IL-7Ralpha) signaling pathways are prime therapeutic targets because these pathways harbor genetic aberrations in both T-cell ALL and B-cell precursor ALL. Therapeutic targeting of the IL-7Ralpha signaling pathways may lead to improved outcomes in a subset of patients. PMID: 27268088 These studies provide in vivo evidence that IL-7Ralpha signals positively regulate normal human B-cell production and proliferation beyond the fetal period and suggest that TSLP can replace IL-7 in providing these signals. PMID: 27325567 Study showed that a genetic variant in the 5' UTR of DDX39B reduces translation of DDX39B mRNAs and increases multiple sclerosis (MS) risk. Importantly, this DDX39B variant showed strong genetic & functional epistasis with allelic variants in IL7R exon 6; study establishes the occurrence of biological epistasis in humans & provides mechanistic insight into the regulation of IL7R exon 6 splicing & its impact on MS risk. PMID: 28340352 study identifies casein kinase 2 as a major player in the effects of interleukin-7 and interleukin-7 receptor in T-cell acute lymphoblastic leukemia PMID: 27470599 IL7Ralpha level was higher in asthmatic than in nonasthmatic children. PMID: 26999524 Meta-analysis demonstrated that the IL7R T244I polymorphism was associated with susceptibility to multiple sclerosis PMID: 27456877 This study suggested that the IL7R C allele was associated with an increased risk of MS and larger-scale studies of populations are needed to explore the roles played by the IL7R T244I polymorphism during the pathogenesis of multiple sclerosis. PMID: 27188999 the variant of IL-7RA (rs6897932) was associated with NMO especially NMO-IgG positive patients while the variant of IL-7 (rs1520333) with MS patients. PMID: 26608987 this study shows that in human CD8 T cells that IL-7 binding to its receptor induces CD127 internalization via clathrin-mediated endocytosis, transport of the receptor from early to late endosomes and ultimately degradation of CD127 by the proteasome. PMID: 26272555 Immunophenotyping with CD135 and CD117 predicts the FLT3, IL-7R and TLX3 gene mutations in childhood T-cell acute leukemia. PMID: 26852660 results suggest that LNK suppresses IL-7R/JAK/STAT signaling to restrict pro-/pre-B progenitor expansion and leukemia development, providing a pathogenic mechanism and a potential therapeutic approach for B-ALLs with LNK mutations. PMID: 26974155 Pretransplant recipient circulating CD4+CD127lo/-TNFR2+ Treg cell is potentially a simpler alternative to Treg cell function as a pretransplant recipient immune marker for acute kidney injury. PMID: 26425877 Data suggest that the lack of IL-7 receptor alpha chain (IL7Ralpha) expression, associated with hypermethylation of the IL7R promoter, in T cells restricts T-cell development in Schimke immuno-osseous dysplasia (SIOD) patients. PMID: 26499378 Substitution of the first 10 Nterminal residues or deletion of residues 17-21 prevented Tat from interacting with and down regulating CD127 from the cell surface. PMID: 25986373 cooperation between IL-7R and alpha2beta1 integrin can represent an important pathogenic pathway in Th17-osteoclast function associated with inflammatory diseases PMID: 26408663 This study then provides evidence that soluble factor(s) are responsible for low CD127 expression on circulating CD8 T-cells in HIV+ individuals and further implicates Tat in suppressing this receptor essential to CD8 T-cell proliferation and function. PMID: 25033393 Findings indicate that IL7 receptor (IL7R) and c-myc expression as intrinsic biomarkers that can predict the fate of CD8+ T effector cells after adoptive transfer. PMID: 26100671 A girl with severe combined immunodeficiency was a compound heterozygote for 2 new frameshift mutations, c.589_598del10 p.P197fsX44 and c.993delA p.Q331fsX2. Each parent carried one of these. PMID: 26123418 the influence of IL-7 receptor alpha-chain (IL-7Ralpha) gene haplotypes in donors on the outcome of haematopoietic cell transplantation, was investigated. PMID: 25352021 Data indicate that individuals carrying the interleukin-7 receptor A/A genotype were more susceptible to diarrhea. PMID: 25366767 This study demonstrated that rs6897932 in IL7Ra gene is associated with the progression of multiple sclerosis in Central European Slovak population. PMID: 25903732 The present study highlights perturbed IL-7/IL-7R T cell signaling through STAT5 as a potential mechanism of T cell exhaustion in chronic T. cruzi infection. PMID: 25769928 rs6897932 allele of interleukin-7 receptor alpha is not associated with general inflammation, and reported associations between T-allele in rs6897932 with several autoimmune diseases may be mediated through effects on restricted part of the immune system PMID: 25421942 Sustained virological response correlates with higher expression of CD127, lower T cell exhaustion status and better HIV and HCV proliferative responses at baseline. PMID: 25007250 Our analysis revealed that none of these 35 samples carried an IL7R mutation in exon 6. Whether differences in the genetic makeup of adult and childhood T-ALL explain the differential response to therapy remains to be determined PMID: 24678068 CD127(hi) effluxer CD8(+) T cells represent a novel population of early memory T cells resident in BM with properties required for long-lived memory PMID: 25231631 Early Omenn syndrome can be observed in patients with IL7R mutations, and autoimmune cytopenias could also complicate the clinical course of SCID babies with this type of defect. PMID: 24759676 CD8 positive T lymphocytes expressing CD127 exhibit increased distribution frequency and distinct functional characteristics that correlate with clinicopathological status in oral neoplasms. PMID: 24465587 Il7 receptor SNP rs6897932 may be associated with increased systemic lupus erythematosus risk in Chinese populations. PMID: 24242875 data suggest that lnc-IL7R contributes another layer of complexity in regulation of the inflammatory response PMID: 24723426 Measurement of the sIL-7R/IL-7 axis will help in guided immune monitoring after HSCT and guided interference with sIL-7R may be explored in GVHD management. PMID: 24946690 we report noncysteine in-frame mutations in IL7R and CRLF2 located in a region of the transmembrane domain closer to the cytosolic domain in acute lymphoblastic leukemias PMID: 24787007 the results indicate that the genetic variation of IL7R may be associated with inflammatory demyelinating diseases such as Multiple sclerosis (MS) and neuromyelitis optica (NMO) in the population studied. PMID: 24337176 CD127 expression is differentially modulated on CD4+ and CD8+ T-cells in the course of T1D. PMID: 24348676 The findings indicate that genetic variants of IL7RA result in haplotype-associated differential responsiveness to immunological stimuli that influence multiple sclerosis susceptibility not exclusively by varying levels of soluble IL-7RA. PMID: 23985573 The IL7R, TNFRSF1A, and GPC5 polymorphisms tended to be associated with having a second event of Multiple sclerosis within a year. PMID: 24130709 The IL7R polymorphism was associated with reduced odds of multiple sclerosis attacks involving the brainstem/cerebellum as were the TNFRSF1A and IL12A polymorphisms. PMID: 24130718

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.