Recombinant Human IL-1 beta/IL-1F2 Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0013
Recombinant Human IL-1 beta (2 ug/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band at 17 kDa and purity greater than 98%.
Recombinant Human IL-1 beta (2 ug/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band at 17 kDa and purity greater than 98%.

Recombinant Human IL-1 beta/IL-1F2 Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0013
/
Size

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Product Overview

Product Name Recombinant Human IL-1 beta/IL-1F2 Protein
Target Symbol IL1B
Alternative Names Interleukin 1 beta, IL1B, IL1F2, Catabolin, IFN beta Inducing Factor, Osteoclast Activating Factor, OAF
Accession Number P01584
Expression System E.Coli.
Molecular Weight 17.3 kDa (predicted)
Sequence Ala117-Ser269
Purity >98% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining.
Activity Determined by a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically <10 pg/mL.
Formulation Lyophilized from sterile PBS with Trehalose, pH 7.4
Endotoxin Level <0.05 EU/1ug of the protein as determined by the LAL method.
Shipping Shipped at ambient temperature.
Stability & Storage 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.
Reconstitution Reconstitute at 100 ug/mL in sterile PBS.
Target Function Interleukin-1 beta (IL-1 beta) is a pro-inflammatory cytokine that mediates inflammation and triggers other cytokines, such as tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6). IL-1 beta helps regulate the immune response by activating immune cells such as T cells (Th1 and Th17), B cells, and macrophages. It enhances the antigen-presenting function of antigen-presenting cells (APCs). IL-1 beta has pyrogenic effects triggered through the hypothalamus. In cell culture, IL-1 beta maintains the survival of mesenchymal stromal cells and hematopoietic progenitor cells and stimulates the differentiation of mesenchymal stem cells and neural stem cells. IL-1beta signaling is triggered by binding to IL-1RI following by association with the accessory protein, IL-1 RAcP. IL-1 beta signaling is modulated by binding to two decoy receptors a soluble IL-1Ra or membrane-bound IL-1RII.
Tissue Specificity IL-1 beta expression has been identified in endothelial tissue, skin, lungs, gastrointestinal tissue, microglia, astrocytes, neurons, and adipose tissue.
Cellular Localization Secreted protein
Involvement In Disease Dysregulation of IL-1 beta is associated with inflammatory disorders (Cryopyrin-Associated Periodic Syndromes (CAPS), Neonatal-Onset Multisystem Inflammatory Disease (NOMID)), autoimmune disorders (Rheumatoid Arthritis (RA), Systemic Lupus Erythematosus (SLE), Inflammatory Bowel Disease (IBD)), osteoarthritis, Juvenile Idiopathic Arthritis (JIA), psoriasis, dermatitis, Alzheimer’s Disease, Parkinson’s Disease, Multiple Sclerosis (MS), Type 2 Diabetes, Obesity, myocardial infarction, atherosclerosis and cancers by promoting inflammation in the tumor microenvironment and metastasis.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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