Recombinant Human IKK beta Protein

Beta LifeScience SKU/CAT #: BL-0336SG
Recombinant Human IKK beta Protein

Recombinant Human IKK beta Protein

Beta LifeScience SKU/CAT #: BL-0336SG
Catalog No.: BL-0336SG

Product Overview

Tag GST
Host Species Human
Accession NM_001556
Synonym IKK2, IKBKB, IKKB, NFKBIKB, FLJ40509, IKK-beta, MGC131801
Background IKKbeta is a serine/threonine protein kinase that phosphorylates the I-kappa-B protein which is an inhibitor of the transcription factor NF-kappa-B complex. Phosphorylation of I-kappa-B protein triggers the degradation of the inhibitor via the ubiquitination pathway, thereby activating NF-kappa-B complex. The activity of IKKbeta is stimulated by TNF and IL1 and IKKbeta forms a heterodimer that interacts with NIK (1). Overexpression of catalytically inactive IKKbeta blocks cytokine-induced NF-kappa-B activation. Aspirin and sodium salicylate can specifically inhibit IKKbeta activity in vitro and in vivo by binding to IKKbeta to reduce ATP binding (2).
Description Recombinant human IKKbeta (1-662) was produced by baculovirus in Sf9 insect cells, fused with a GST tag at N-terminus. This protein is purified with our unique purification methods.
Source Sf9 insect cells
AA Sequence 1-662
Molecular Weight ~105 kDa
Purity For specific purity information on a given lot, see related COA.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Active
Formulation Recombinant protein is supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol.
Stability The recombinant protein is stable for up to 12 months at -70°C
Usage For Research Use Only
Storage Recombinant Human IKK beta Protein should be stored should be stored at < -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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