Recombinant Human IKK beta Protein
Beta LifeScience
SKU/CAT #: BL-0336SG
Recombinant Human IKK beta Protein
Beta LifeScience
SKU/CAT #: BL-0336SG
Catalog No.: BL-0336SG
Product Overview
| Tag | GST |
| Host Species | Human |
| Accession | NM_001556 |
| Synonym | IKK2, IKBKB, IKKB, NFKBIKB, FLJ40509, IKK-beta, MGC131801 |
| Background | IKKbeta is a serine/threonine protein kinase that phosphorylates the I-kappa-B protein which is an inhibitor of the transcription factor NF-kappa-B complex. Phosphorylation of I-kappa-B protein triggers the degradation of the inhibitor via the ubiquitination pathway, thereby activating NF-kappa-B complex. The activity of IKKbeta is stimulated by TNF and IL1 and IKKbeta forms a heterodimer that interacts with NIK (1). Overexpression of catalytically inactive IKKbeta blocks cytokine-induced NF-kappa-B activation. Aspirin and sodium salicylate can specifically inhibit IKKbeta activity in vitro and in vivo by binding to IKKbeta to reduce ATP binding (2). |
| Description | Recombinant human IKKbeta (1-662) was produced by baculovirus in Sf9 insect cells, fused with a GST tag at N-terminus. This protein is purified with our unique purification methods. |
| Source | Sf9 insect cells |
| AA Sequence | 1-662 |
| Molecular Weight | ~105 kDa |
| Purity | For specific purity information on a given lot, see related COA. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Active |
| Formulation | Recombinant protein is supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol. |
| Stability | The recombinant protein is stable for up to 12 months at -70°C |
| Usage | For Research Use Only |
| Storage | Recombinant Human IKK beta Protein should be stored should be stored at < -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
