Recombinant Human IFNA4 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2524
Recombinant Human IFNA4 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2524
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Product Overview
Tag | Fc |
Host Species | Human |
Accession | NP_066546.1 |
Synonym | IFN-alpha4a, INFA4, MGC142200 |
Background | Interferon, alpha 4 (IFNA4) belongs to the alpha/beta interferon family. Two variants of IFNA4 (IFNA4a and IFNA4b) are known, which differ from each other by changes in their coding regions at nucleotide positions 22 and 41 and can be distinguished by selective restriction enzyme analysis. Interferons are produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. IFN-alpha, the first cytokine to be produced by recombinant DNA technology, has emerged as an important regulator of growth and differentiation, affecting cellular communication and signal transduction pathways as well as immunological control. Originally discovered as an antiviral substance, the efficacy of IFN-alpha in malignant, viral, immunological, angiogenic, inflammatory, and fibrotic diseases suggests a spectrum of interrelated pathophysiologies. IFN-alpha emerged as a prototypic tumor suppressor protein that represses the clinical tumorigenic phenotype in some malignancies capable of differentiation. |
Description | A DNA sequence encoding the mature form of human IFN-alpha4a (NP_066546.1) (Cys 23-Asp 189) was expressed with the N-terminal fused Fc region of human IgG1. |
Source | HEK293 |
Predicted N Terminal | Glu |
AA Sequence | Cys 23-Asp 189 |
Molecular Weight | The recombinant human Fc/IFN-alpha4a is a disulfide-linked homodimeric protein. The reduced monomer consists of 403 a.a. and has a predicted molecular mass of 46 kDa. As a result of glycosylation, the apparent molecular mass of rh Fc/IFN-alpha4a monomer is approximately 50 kDa in SDS-PAGE under reducing conditions. |
Purity | >97% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus (VSV).The EC50 for this effect is 0.2-1 ng/mL. |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |