Recombinant Human Hyper IL-6 Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0002UL
Recombinant Human Hyper IL-6 (2 µg/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue, showing a single band at 56-60 kDa and purity greater than 98%.
Recombinant Human Hyper IL-6 (2 µg/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue, showing a single band at 56-60 kDa and purity greater than 98%.

Recombinant Human Hyper IL-6 Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0002UL
/
Size

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Product Overview

Product Name Recombinant Human Hyper IL-6 Protein
Target Symbol IL6R
Alternative Names Interleukin 6, Interferon Beta-2, IFN-Beta-2, IFNB2, B-Cell Stimulatory Factor 2, BSF2, Hybridoma Growth Factor, HGF, HSF, CTL Differentiation Factor, CDF
Accession Number P08887 (IL-6 R) & P05231 (IL-6)
Expression System CHO cell line
Molecular Weight 45.9 kDa (predicted), 42 kDa (observed on SDS-PAGE gel stained with Coomassie® Blue)
Sequence sIL6R (Val112-Ala323)-GSL-IL6（Val30-Met212）
Purity >98% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining.
Activity This IL-6/IL-6R fusion protein stimulates proliferation in DS-1 cells (IL-6R+), albeit less than IL-6 alone. Also, IL-6/IL-6R fusion protein inhibits the proliferation of HUVECs (IL-6R-). Determined by a cell proliferation assay using DS-1 mouse lymphoblast cells, the ED50 for this effect is typically <750 pg/mL. Also determined by an inhibition of proliferation assay using HUVECs, the ED50 for this effect is typically 1-10 ng/mL.
Formulation Lyophilized from sterile PBS with Trehalose, pH 7.4
Endotoxin Level <0.005 EU/1ug of the protein as determined by the LAL method.
Shipping Shipped at ambient temperature.
Stability & Storage 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.
Reconstitution Reconstitute at 100 ug/mL in sterile PBS. If desired, 5% Trehalose or 0.1% BSA may be added.
Target Function IL-6 plays a role in regulating immune response, inflammation, hematopoiesis, metabolism, and neurological processes. IL-6 influences the growth and differentiation of hematopoietic progenitor and stem cells. In the innate immune response, IL-6 is released by macrophages and dendritic cells. During the adaptive immune response, IL-6 plays a role in B cell maturation and differentiation of T cells into T follicular helper (Tfh) cells and Th17 cells. Muscle tissue and the central nervous system response to IL-6 has been linked to lipolysis and modulation of insulin response. IL-6 signaling occurs via binding to IL-6R alpha (CD126) and subsequent dimerization with the signal-transducing gp130 subunit.
Tissue Specificity IL-6 expression has been reported in muscle tissue, endothelial tissue, lung, intestine, and adipose tissue.
Cellular Localization Secreted protein
Involvement In Disease Dysregulation of IL-6 is implicated in rheumatoid arthritis, systemic juvenile idiopathic arthritis (sJIA) inflammatory bowel disease (IBD), autoimmune hepatitis, atherosclerosis, insulin resistance, type 2 diabetes, fatty liver disease, obesity-related inflammation, Castleman disease, Behçet’s disease Alzheimer’s disease, systemic lupus erythematosus, multiple myeloma, and various cancers (prostate, breast, and lung) through actions on the tumor microenvironment.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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