Recombinant Human Histidine Decarboxylase (HDC) Protein (His)

Name: Recombinant Human Histidine Decarboxylase (HDC) Protein (His)
Brand: Beta LifeScience
SKU: BLC-10862P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Histidine Decarboxylase (HDC) Protein (His) is produced by our Yeast expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P19113
Target Symbol	HDC
Synonyms	DCHS_HUMAN; EC 4.1.1.22; HDC; Histidine decarboxylase
Species	Homo sapiens (Human)
Expression System	Yeast
Tag	N-6His
Target Protein Sequence	MMEPEEYRERGREMVDYICQYLSTVRERRVTPDVQPGYLRAQLPESAPEDPDSWDSIFGDIERIIMPGVVHWQSPHMHAYYPALTSWPSLLGDMLADAINCLGFTWASSPACTELEMNVMDWLAKMLGLPEHFLHHHPSSQGGGVLQSTVSESTLIALLAARKNKILEMKTSEPDADESCLNARLVAYASDQAHSSVEKAGLISLVKMKFLPVDDNFSLRGEALQKAIEEDKQRGLVPVFVCATLGTTGVCAFDCLSELGPICAREGLWLHIDAAYAGTAFLCPEFRGFLKGIEYADSFTFNPSKWMMVHFDCTGFWVKDKYKLQQTFSVNPIYLRHANSGVATDFMHWQIPLSRRFRSVKLWFVIRSFGVKNLQAHVRHGTEMAKYFESLVRNDPSFEIPAKRHLGLVVFRLKGPNCLTENVLKEIAKAGRLFLIPATIQDKLIIRFTVTSQFTTRDDILRDWNLIRDAATLILSQHCTSQPSPRVGNLISQIRGARAWACGTSLQSVSGAGDDPVQARKIIKQPQRVGAGPMKRENGLHLETLLDPVDDCFSEEAPDATKHKLSSFLFSYLSVQTKKKTVRSLSCNSVPVSAQKPLPTEASVKNGGSSRVRIFSRFPEDMMMLKKSAFKKLIKFYSVPSFPECSSQCGLQLPCCPLQAMV
Expression Range	1-662aa
Protein Length	Full Length
Mol. Weight	76.2kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Catalyzes the biosynthesis of histamine from histidine.
Protein Families	Group II decarboxylase family
Database References	HGNC: 4855 OMIM: 142704 KEGG: hsa:3067 STRING: 9606.ENSP00000267845 UniGene: PMID: 27769832 a Possible Role for the Histidine Decarboxylase Gene in Tourette Syndrome in the Chinese Han Population PMID: 27529419 Human enterochromaffin-like cells appear to partially co-express VMAT-2 and HDC. Co-expression of VMAT-2 and HDC might be required for increased histamine production in patients with gastric neuroendocrine tumors. PMID: 26715806 The findings indicate that polymorphisms of HDC gene were significantly associated with breast cancer in Chinese Han population and may be novel diagnostic or therapeutic targets for breast cancer. PMID: 24835231 HDC rs17740607 polymorphism is at least a partial loss-of-function variant and acts as a protective factor against chronic heart failure PMID: 25846768 Investigated variation across the HDC (histidine decarboxylase) gene for association with Tourette Syndrome. PMID: 23825391 HDC production in the stomach is associated with bile acid exposure and its related transcriptional regulation network of FXR, SHP, and CDX1. PMID: 24415870 Data indicate that histidine decarboxylase (HDC) is expressed by neutrophils. PMID: 23572231 Structural study reveals that Ser-354 determines substrate specificity on human histidine decarboxylase. PMID: 22767596 crystal of histidine decarboxylase belonged to space group C2, with unit-cell parameters a = 215.16, b = 112.72, c = 171.39 A, beta = 110.3 degrees PMID: 22684068 It was shown that for serum and urine, HDC levels achieved sensitivities and specificities compatible to or even greater than those of established biomarkers for the diagnosis of intestinal mucosal injury in patients with acute intestinal obstruction. PMID: 21915437 The novel concept that an autocrine loop, consisting of enhanced histamine synthesis by histidine decarboxylase, sustains cholangiocarcinoma growth is proposed. PMID: 21873469 Variants in the HDC gene may play little or no role in Tourette Syndrome susceptibility in Chinese Han population. PMID: 22095709 Studies provide lead compounds for inhibitors of ornithine decarboxylase and human histidine decarboxylase. PMID: 21454364 The histidine decarboxylase allele Glu644 in homozygosity increases the risk of developing rhinitis in the studied population PMID: 20608921 identification of a rare functional mutation in the HDC gene encoding L-histidine decarboxylase, the rate-limiting enzyme in histamine biosynthesis which points to its role in Tourette's syndrome and tics PMID: 20445167 the elevation of HDC expression during human monocytic differentiation PMID: 11556524 HDC expression is regulated by gastrin through a complex cis-acting element, which binds at least three distinct nuclear factors PMID: 12372397 Histidine decarboxylase promoter activity can be repressed by kruppel-like factor 4 PMID: 14670968 histidine decarboxylase mRNA is found in human vascular smooth muscle and endothelial cells PMID: 15167966 HDC and histamine content were significantly higher in the tumor specimens PMID: 15928846 Chronic urticaria expresses high levels of HDC as compared to normal foreskin, breast skin and cultured human keratinocytes. PMID: 16297195 HDC expression and histamine production are increased in the superficial kidney cortex zone during pregnancy PMID: 16760908 Defective levels found in normal placenta compared with pre-eclampsia labor. PMID: 16822545 Synthesis of histamine was found to be restricted to the basophil compartment of the CML clone and to depend on signaling through the PI3-kinase pathway. CML cells also expressed histamine receptors PMID: 16849647 HDC SNP's are significantly associated with age at natural menopause in Caucasian women. PMID: 16919600 Histamine decarboxylase expressed in endothelial cells of microvessels might play a role in regulating angiogenesis in esophageal squamous cell carcinomas. PMID: 19133005 HFR was regulated by HIF-1 activation. Depletion of HIF-1alpha prevents hypoxic induction of HDC in BMMCs. Hypoxia induces HDC expression by transcriptional mechanisms dependent upon HIF-1. PMID: 19266161 the apparent occurrence of an unusual TG 3' splice site in intron 4 is discussed PMID: 17672918

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.