Recombinant Human Heterogeneous Nuclear Ribonucleoprotein K (HNRNPK) Protein (GST)

Name: Recombinant Human Heterogeneous Nuclear Ribonucleoprotein K (HNRNPK) Protein (GST)
Brand: Beta LifeScience
SKU: BLC-08483P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Heterogeneous Nuclear Ribonucleoprotein K (HNRNPK) Protein (GST) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P61978
Target Symbol	HNRNPK
Synonyms	CSBP; dC stretch binding protein; FLJ41122; Heterogeneous nuclear ribonucleoprotein K; hnRNP K; HNRNPK; HNRPK; HNRPK_HUMAN; Transformation up regulated nuclear protein; Transformation up-regulated nuclear protein; Transformation upregulated nuclear protein; TUNP
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-GST
Target Protein Sequence	TEQPEETFPNTETNGEFGKRPAEDMEEEQAFKRSRNTDEMVELRILLQSKNAGAVIGKGGKNIKALRTDYNASVSVPDSSGPERILSISADIETIGEILKKIIPTLEEGLQLPSPTATSQLPLESDAVECLNYQHYKGSDFDCELRLLIHQSLAGGIIGVKGAKIKELRENTQTTIKLFQECCPHSTDRVVLIGGKPDRVVECIKIILDLISESPIKGRAQPYDPNFYDETYDYGGFTMMFDDRRGRPVGFPMRGRGGFDRMPPGRGGRPMPPSRRDYDDMSPRRGPPPPPPGRGGRGGSRARNLPLPPPPPPRGGDLMAYDRRGRPGDRYDGMVGFSADETWDSAIDTWSPSEWQMAYEPQGGSGYDYSYAGGRGSYGDLGGPIITTQVTIPKDLAGSIIGKGGQRIKQIRHESGASIKIDEPLEGSEDRIITITGTQDQIQNAQYLLQNSVKQYADVEGF
Expression Range	3-464aa
Protein Length	Partial of Isoform 2
Mol. Weight	78.2 kDa
Research Area	Immunology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction. As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest.
Subcellular Location	Cytoplasm. Nucleus, nucleoplasm. Cell projection, podosome. Note=Recruited to p53/TP53-responsive promoters, in the presence of functional p53/TP53 (PubMed:16360036). In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear (PubMed:18775702).
Database References	HGNC: 5044 OMIM: 600712 KEGG: hsa:3190 STRING: 9606.ENSP00000365439 UniGene: PMID: 29921878 Study findings underscore the biological significance of MRPL33-L and hnRNPK in the tumor formation and identifies hnRNPK as a critical splicing regulator of MRPL33 pre-mRNA in cancer cells. PMID: 28869607 hnRNPK regulates PLK1 expression by competing with the PLK1-targeting miRNAs, miR-149-3p and miR-193b-5p. PMID: 28708135 we discovered that hnRNPK plays an important role in bladder cancer, suggesting that it is a potential prognostic marker and a promising target for treating bladder cancer. PMID: 27862976 The hnRNPK positively regulates the level of prostate tumor overexpressed 1-antisense 1 which harbors five binding sites for miR-1207-5p. The knockdown of hnRNPK or PTOV1-AS1 increased the enrichment of heme oxygenase-1 mRNA in miR-1207-5p-mediated miRNA-induced silencing complex and thus suppressed the expression of heme oxygenase-1. PMID: 28228215 High HNRNPK expression is associated with pancreatic cancer. PMID: 28423622 Down-regulation of DAB2IP correlated negatively with hnRNPK and MMP2 expressions in CRC tissues. In conclusion, our study elucidates a novel mechanism of the DAB2IP/hnRNPK/MMP2 axis in the regulation of CRC invasion and metastasis, which may be a potential therapeutic target. PMID: 28335083 Tumor cells bearing a p53 mutation showed increased damage levels and delayed repair. Knockdown of hnRNPK applied simultaneously with irradiation reduced colony-forming ability and survival of tumor cells. Taken together, our data shows that hnRNPK is a relevant modifier of DNA damage repair and tumor cell survival. We therefore recommend further studies to evaluate the potential of hnRNPK as a drug target for improvement PMID: 28426877 Data suggest that hnRNPK plays role in heat shock response of cells by regulating HSF1; hnRNPK inhibits HSF1 activity, resulting in reduced expression of HSP27 and HSP70 mRNAs; hnRNPK also down-regulates binding of HSF1 to heat shock response element. (hnRNPK = heterogeneous-nuclear ribonucleoprotein K; HSF1 = heat shock transcription factor 1; HSP = heat-shock protein) PMID: 28592492 KRAS-mutant colorectal carcinoma shows intrinsic radioresistance along with rapid upregulation of hnRNP K in response to ionizing radiation that can effectively be targeted by MEK inhibition. PMID: 27793696 Nujiangexathone A, a novel compound from Garcinia nujiangensis, down-regulates hnRNPK levels in cervical tumor cells, inducing cell cycle arrest. PMID: 27424288 Our study also suggests that loss of function variants in HNRNPK should be considered as a molecular basis for patients with Kabuki-like syndrome PMID: 26954065 these findings support a critical role for hnRNP K in the regulation of autophagy in drug-resistant leukemia cells, and this RNP is therefore a potential target of clinical drug-resistance treatment. PMID: 27155326 hnRNP K is a multifunctional protein that can regulate both oncogenic and tumor suppressive pathways through a plethora of chromatin-, DNA-, RNA-, and protein-mediated activates, suggesting its aberrant expression may have broad-reaching cellular impacts. (Review) PMID: 27049467 These results establish the role of hnRNP K and PCPB1 in the translational control of morphine-induced MOR expression in human neuroblastoma (NMB) cells as well as cells stably expressing MOR (NMB1). PMID: 27292014 HnRNP K is a promising tissue biomarker for diagnosing gastric cancer. PMID: 27278897 CASC11 can target heterogeneous ribonucleoprotein K (hnRNP-K) to activate WNT/beta-catenin signaling in colorectal cancer cells to promote tumor growth and metastasis. PMID: 27012187 Data show that heterogeneous nuclear ribonucleoprotein K (hnRNPK) stabilized of cellular FLICE-inhibitory protein (c-FLIP) protein through inhibition of glycogen synthase kinase 3 beta (GSK3beta) Ser9 phosphorylation during the TNF-related aoptosis-inducing ligand (TRAIL)-induced apoptosis. PMID: 26972480 HhnRNP-K-mediated regulation of NMHC IIA mRNA translation contributes to the control of enucleation in erythropoiesis. PMID: 26823606 hnRNP K binds miR-122, a mature liver-specific microRNA required for Hepatitis C virus replication. PMID: 26330540 The authors identified two new human proteins that interact with Ehrlichia chaffeensis EtpE-C: CD147 and heterogeneous nuclear ribonucleoprotein K (hnRNP-K). PMID: 26530384 Data show that proto-oncogene protein c-myc is upregulated by sumoylated heterogeneous nuclear ribonucleoprotein K (hnRNP K) at the translational level in Burkitt's lymphoma cells. PMID: 26317903 hnRNP K may be a key molecule involved in cell motility in RCC cells PMID: 26713736 It describes the identification of heterogeneous nuclear ribonucleoprotein K (hnRNPK) as one of the composite element binding factors(CEBF) that acts as transactivator of PXR promoter. PMID: 26586566 RTVP-1 regulates glioma cell spreading, migration and invasion and that these effects are mediated via interaction with N-WASP and by interfering with the inhibitory effect of hnRNPK on the function of this protein. PMID: 26305187 we investigate the role of hnRNP K in the radioresistance of malignant melanoma cells PMID: 26136337 These results indicate that dengue virus type 2 and Junin virus induce hnRNP K cytoplasmic translocation to favor viral multiplication. PMID: 25865411 Data implicate hnRNPK in the development of hematological disorders and suggest hnRNPK acts as a tumor suppressor. PMID: 26412324 Inhibition of CDK2 phosphorylation blocked phosphorylation of hnRNP K, preventing its incorporation into stress granules (SGs). Due to interaction between hnRNP K with TDP-43, the loss of hnRNP K from SGs prevented accumulation of TDP-43. PMID: 25410660 HNRNPK might determine efficiency of Hepatitis C virus particle production by limiting the availability of viral RNA for incorporation into virions. PMID: 25569684 Data indicate that twenty proteins were identified as binding partners of the primary activating element in the heterogeneous nuclear ribonucleoprotein K (hnRNP K) promoter. PMID: 25497182 hnRNP K interacts with EV71 5' UTR, which is required for efficient synthesis of viral RNA.[review] PMID: 26164948 hnRNPK is potentially implicated in the radiogenic response of HNSCC. PMID: 25281771 hnRNP K plays an important role in the mitotic process in colon cancer cells.hnRNP K upregulates NUF2 and promotes the tumorigenicity of colon cancer cells. PMID: 25701787 These findings functionally integrate K17, hnRNP K, and gene expression along with RSK and CXCR3 signaling in a keratinocyte-autonomous axis and provide a potential basis for their implication in tumorigenesis PMID: 25713416 HnRNP K can induce MMP12 expression and enzyme activity through activating MMP12 promoter, which promotes cell migration and invasion in nasopharyngeal carcinoma cells. PMID: 24885469 Results indicate that the interaction between the AR and hnRNP K has an important role in the progression of prostate cancer. PMID: 24626777 hnRNP K and PU.1 act synergistically during granulocytic differentiation, hnRNP K seems to have a negative effect on PU.1 activity during monocytic maturation PMID: 25005557 CNBP overexpression caused increase of cell death and suppression of cell metastasis through its induction of G-quadruplex formation in the promoter of hnRNP K resulting in hnRNP K down-regulation PMID: 24594223 Data show that SET accumulation up-regulated hnRNPK mRNA and total/phosphorylated protein, promoted hnRNPK nuclear location, and reduced Bcl-x mRNA levels. PMID: 24508256 NS1-BP-hnRNPK complex is a key mediator of influenza A virus gene expression. PMID: 23825951 These studies demonstrate hnRNP K to be a multifunctional protein that supports vesicular stomatitis virus infection via its role(s) in suppressing apoptosis of infected cells. PMID: 23843646 hnRNPK may play a role in recruitment of XRN2 to gene loci thus regulating coupling 3'-end pre-mRNA processing to transcription termination. PMID: 23857582 heterogeneous nuclear ribonucleoprotein K (hnRNPK), a protein known to integrate multiple signal transduction pathways with gene expression, as a SERT distal polyadenylation element binding protein PMID: 23798440 Prolonged downregulation of hnRNP K using small interfering RNA significantly decreased cell viability and increased apoptosis in HCC cell lines in a p53-independent manner. PMID: 23455382 Caspase-3 cleaves hnRNP K in erythroid differentiation. PMID: 23519117 HnRNP K controlled expression of IE2 protein of Human cytomegalovirus during viral replication. PMID: 23099853 hnRNP-K regulates extracellular matrix, cell motility, and angiogenesis pathways. Involvement of the selected genes (Cck, Mmp-3, Ptgs2, and Ctgf) and pathways was validated by gene-specific expression analysis PMID: 23564449 ATM-dependent phosphorylation of heterogeneous nuclear ribonucleoprotein K promotes p53 transcriptional activation in response to DNA damage. PMID: 23343766 SUMO modification plays a crucial role in the control of hnRNP-K's function as a p53 co-activator in response to DNA damage by UV PMID: 23092970

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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